A Laboratory Guide to the Mammalian Embryo by David K. GardnerA Laboratory Guide to the Mammalian Embryo by David K. Gardner

A Laboratory Guide to the Mammalian Embryo

EditorDavid K. Gardner, Michelle Lane, Andrew J. Watson

Spiral Bound | June 2, 2004

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This book pulls together the full range of cell culture, biochemical, microscopic, and genetic techniques to study the early mammalian embryo. Until now, there has never been such a comprehensive compendium, though there have been more focused books of protocol, such as Manipulating the MouseEmbryo, from Cold Spring Harbor. This book is intended to appeal to all constituencies, from basic experimental science to clinical and animal science applications.
David K. Gardner is Director of Research and Development and Michelle Lane is a Senior Scientist, both at the Colorado Center for Reproductive Medicine. Andrew J. Watson is an Associate Professor, Department of Physiology, University of Western Ontario.
Title:A Laboratory Guide to the Mammalian EmbryoFormat:Spiral BoundDimensions:432 pages, 8.31 × 10.79 × 0.91 inPublished:June 2, 2004Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0195142268

ISBN - 13:9780195142266


Table of Contents

PrefaceAcknowledgementsList of ContributorsAbbreviations1. Norah Spears, Evelyn E. Telfer and Alison A. Murray: Follicle development in vitro2. Michelle Lane and David Gardner: Preparation of gametes, in vitro maturation, in vitro fertilization and embryo recovery3. David Gardner and Michelle Lane: Culture of the mammalian preimplantation embryo4. Denny Sakkas, Michelle Lane and David Gardner: Assessment of embryo development and viability5. Gianpiero Palermo, Queenie V. Neri, Takumi Takeuchi, Lucinda L. Veeck, Zev Rosenwaks: Micromanipulation : ICSI6. Joyce Harper and Alpesh Doshi: Micromanipulation : Biopsy7. Michelle Lane, Jay Baltz and David Gardner: Analysis of intracellular ions: pH and calcium8. David Gardner and Michelle Lane: Nutrient uptake and metabolite production and enzyme activity and regulation9. Don Rieger: Metabolic Pathway10. Calvin Simerly, Ricardo Moreno, Joao Ramalho-Santos, Laura Hewitson, and Gerald Schatten: Confocal imaging of structural molecules in mammalian gametes11. Leeanda Wilton: Fluorescent in situ hybridization12. W. A. King, D. H. Betts, W. Slimane and P.K. Basur: Assessment of ploidy, telomere length and telomerase activity in oocytes and embryos13. C. Wrenzycki, A.J. Watson, and M. Calder: Microscale RNA isolation from mammalian embryos14. P. A. De Sousa and A. J. Watson: Relative mRNA transcript abundance in early embryos by Reverse Transcription-Polymerase Chain Reaction (RT-PCR)15. Dave Natale: Characterization of novel genes during early development by application of differential display RT-PCR16. K. E. Latham, G. Nourjanova, K. Wigglesworth and J. J. Eppig: Two-dimensional protein gel database analysis of embryos, oocytes, and oocyte-associated granulosa cells17. Lisa C Barcroft, D Holstead Jones-Taggart and Andrew J Watson: Antisense disruption of gene expression in the preimplantation embryo18. M. A. Sirard and C. Robert: Gene subtraction and analysis19. Daniel Brison Anthony D Metcalfe, Debra J Bloor, Helen R Hunter, Gerard Brady, Susan J Kimber: Analysis of apoptosis in the preimplantation embryo20. D. P. Baluch, C. M. Pauken, and D. G. Capco: Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo21. Susan E. Lazendorf, Catherine A. Boyd and Diane L. Wright: Human Embryonic Stem22. Makoto Nagano: Spermatogonial Transplantion23. Paul De Sousa, William Ritchie, Andras Dinnyes, Tim King, Lesley Paterson, and Ian Wilmut: Somatic Cell Nuclear Transfer24. Tetsunori Mukaida and Magosaburo Kasai: Cryobiology; slow freezing vitrification of embryos