Adenovirus Methods and Protocols: Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions, and Phyologenetics by William S. M. WoldAdenovirus Methods and Protocols: Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions, and Phyologenetics by William S. M. Wold

Adenovirus Methods and Protocols: Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions…

EditorWilliam S. M. Wold, Ann E. Tollefson

Paperback | November 10, 2010

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Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise.Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.
Title:Adenovirus Methods and Protocols: Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions…Format:PaperbackDimensions:376 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617378356

ISBN - 13:9781617378355

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Table of Contents

Ann E. Tollefson, Mohan Kuppuswamy, and William S. M. WoldTABLE OF CONTENTSVol. II Topics: Ad proteins and RNA, lifecycle and host interactions, and phylogenetics1. Analysis of the Efficiency of Adenovirus TranscriptionCristina Iftode and S. J. Flint2. The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein DomainsPaul M. Loewenstein, Chao-Zhong Song and Maurice Green 3. Preparation of Soluble Extracts from Adenovirus Infected Cells for Studies of RNA SplicingOliver Mühlemann and Göran Akusjärvi4. In Vitro Methods to Study RNA Interference During an Adenovirus InfectionGunnar Andersson, Ning Xu and Göran Akusjärvi5. Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In SituEileen Bridge6. Study of Nucleolar Localization of Adenovirus Core ProteinsDavid A. Matthews7. Analysis of Adenovirus Infections in Synchronized CellsDavid A. Ornelles, Robin N. Shepard and Felicia D. Goodrum8. Co-Immunoprecipitation of Protein ComplexesPeter Yaciuk9. Chromatin Immunoprecipitation (ChIP) to Study the Binding of Proteins to the Adenovirus Genome in VivoJihong Yang and Patrick Hearing10. Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift AssaysPilar Perez-Romero and Michael J. Imperiale11. Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometry Anuj Gaggar, Dmitry Shayakhmetov, and Andre Lieber12. The Use of Cell Microinjection for the in vivo Analysis of Viral Transcriptional Regulatory Protein DomainsMaurice Green, Andrew Thorburn, Robert Kern and Paul M. Loewenstein13. Determination of the Transforming Activities of Adenovirus OncogenesMichael Nevels and Thomas Dobner14. Human Adenovirus Type 12: Crossing Species Barriers to Immortalize the Viral GenomeWalter Doerfler15. Measurement of NK Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells John M. Routes16. A Flow Cytometric Assay for Analysis of NK Cell-Mediated Cytolysis of Adenovirus-Transformed CellsGraham Bottley, Graham P. Cook, and G. Eric Blair17. Large Scale Purification and Crystallization of Adenovirus Hexon John J. Rux and Roger M. Burnett18. Synthesis and Assay of Recombinant Adenovirus Protease Joseph M. Weber19. Assay for the Adenovirus Proteinase- Purification of the Enzyme and Synthesis of a Fluorogenic SubstrateWalter F. Mangel and William J. McGrath20. Cofactors of the Adenovirus Proteinase: Measuring Equilibrium Dissociation Constants and Stoichiometries of Binding Walter F. Mangel and William J. McGrath21. Characterization of the Adenovirus Fiber ProteinJeffrey A. Engler and Jeong Shin Hong22. Phylogenetic Analysis of Adenovirus SequencesBalázs Harrach and Mária Benkö23. Assessment of Genetic Variability Among Subspecies B1 Human Adenoviruses for Molecular Epidemiology StudiesAdriana E. Kajon and Dean D. Erdman

Editorial Reviews

"...recommend it to investigators working in the area of viral-host interactions." -Doody's Book Review, Weighted Numerical Score: 70 - 3 Stars Reviews of the First edition:"This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them. . .a very comprehensive methods book that will be a laboratory standard for years to come."- Microbiology Today". . .this book constitutes an excellent reference text . . .this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses."-FEBS Letters