Affinity Separations: A Practical Approach by Paul MatejtschukAffinity Separations: A Practical Approach by Paul Matejtschuk

Affinity Separations: A Practical Approach

EditorPaul Matejtschuk

Paperback | June 1, 1997

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Affinity Separations: A Practical Approach is a new collection of affinity-based purification protocols with an international authorship from across the life sciences. The introductory chapter outlines recent advances in ligand-coupling, matrix choice, and operating modes. The second chapterdescribes methods for obtaining quantitative data from affinity processes. There are specific chapters describing methods for the affinity separation of proteins, nucleic acids, and oligosaccharides. Methods for immunoglobulin purification and immunoaffinity protocols are discussed in a furtherchapter, and the use of affinity tags in the recovery of engineered proteins in another. Finally, modern techniques for cell separations are outlined. The book presents detailed laboratory protocols together with troubleshooting advice and reviews of recent developments. It is aimed at benchscientists, both those in analytical research laboratories, and those non-specialists looking to apply affinity methods in their work.
Paul Matejtschuk is at BioProducts Laboratory, Hertfordshire.
Title:Affinity Separations: A Practical ApproachFormat:PaperbackDimensions:272 pages, 9.21 × 6.14 × 0.63 inPublished:June 1, 1997Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199635501

ISBN - 13:9780199635504

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Table of Contents

1. Recent developments in affinity separation technologies2. Quantitative affinity chromatography3. Affinity separations of proteins4. Affinity separation of nucleic acids5. Affinity chromatography of oligosaccharides and glycopeptides6. Immunoaffinity and IgG receptor technologies7. Designer affinity purifications of recombinant proteins8. Affinity separations of cellsAppendix: list of suppliers

Editorial Reviews

"While the editor suggests that it is not intended as a textbook, this book would certainly provide a valuable additional source for courses on bioseparations. However, its most welcome reception will come from laboratory scientists at all levels..."--Journal of Medicinal Chemistry"This publication is designed to provide novice laboratory technicians and researchers with the background and examples necessary to develop and apply an affinity purification scheme. Built around practical examples, the chapters provide step-by-step protocols for the affinity separation ofproteins, nucleic acids, oligosaccharides, immunoglobulin purification, and immunoaffinity applications. The eight individually authored chapters are further enhanced with background and troubleshooting information as well as charts, graphs, example results, and references." -- Biosis, Vol 50, Issue10, Oct 22, 1998