Aging Methods and Protocols by Yvonne A. BarnettAging Methods and Protocols by Yvonne A. Barnett

Aging Methods and Protocols

EditorYvonne A. Barnett, Christopher R. Barnett

Paperback | November 9, 2010

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Yvonne and Christopher Barnett and a team of recognized international experts detail key biochemical, analytical, and molecular techniques for the investigation of aging at the cellular, tissue, organ, and whole system levels. These cutting-edge methods address a wide range of research needs, from uncovering the factors associated with cell senescence and death, to exploring alterations in the body's ability both to metabolize xenobiotics and to defend itself against biomolecular damage. State-of-the-art protocols also measure the morphological, functional, and molecular changes that accumulate within mitochrondria over time, and permit the genetic and functional characteristics of the immune system to be determined. Wide ranging and highly practical, Aging Methods and Protocols provides today's molecular gerontologists, pharmacologists, and clinical investigators with a gold-standard collection of readily reproducible techniques for identifying those key cellular and molecular processes that might one day make it possible to regulate the aging process.
Title:Aging Methods and ProtocolsFormat:PaperbackDimensions:400 pages, 9.02 × 5.98 × 0.07 inPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617370835

ISBN - 13:9781617370830

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Table of Contents

Understanding Aging, Bernard L. Strehler. Part I. The Study of Cell Senescence and Cell Death. Use of the Fibroblast Model in the Study of Cellular Senescence, Vincent J. Cristofalo, Craig Volker, and Robert G. Allen. Human T-Cell Clones, Graham Pawelec. Telomeres and Replicative Senescence, Hector F. Valenzuela and Rita B. Effros. Detection of Molecular Events During Apoptosis by Flow Cytometry, Ruaidhri J. Carmody, Ana P. Costa-Pereira, Sharon L. McKenna, and Tom G. Cotter. Raf-1 Protein Kinase Activity in T Cells from Aged Mice, Christopher J. Kirk and Richard A. Miller. Identification of Differentially Expressed Genes in Young and Senescent T Cells, Andrea Engel, Mahdi Adibzadeh, and Graham Pawelec. Part II. Age-Related Changes to Xenobiotic Metabolizing Enzyme Systems. Xenobiotic-Metabolizing Enzyme Systems and Aging, Christopher R. Barnett and Costas Ioannides. Part III. Age-Related Changes to Defense Systems Against Biomolecule Damage. Assessing Age-Related Changes in Antioxidant Status: The FRASC Assay for Total Antioxidant Power and Ascorbic Acid Concentration in Biological Fluids, Iris F. F. Benzie and John J. Strain. Measurement of DNA Damage and Repair Capacity as a Function of Age Using the Comet Assay, Peter H. Clingen, Jillian E. Lowe, and Michael H. L. Green. Measurement of DNA Damage and Repair in Human White Blood Cells by an Immunochemical Assay, Govert P. van der Schans. Part IV. Age-Related Changes to Cellular Biomolecules. Measurement of 8-Oxo-deoxyguanosine in Lymphocytes, Cultured Cells, and Tissue Samples by HPLC with Electrochemical Detection, Sharon G. Wood, Catherine M. Gedik, Nicholas J. Vaughan, and Andrew R. Collins. Mutation and the Aging Process: Mutant Frequency at the HPRT Gene Locus as a Function of Age in Humans, Yvonne A. Barnett and Christopher R. Barnett. Somatic Mutations and Aging: Methods for Molecular Analysis of HPRT Mutations, Sai-Mei Hou. Assessment of Susceptibility of Low-Density Lipoprotein to Oxidation, Jane McEneny and Ian S. Young. Measurement of Pentosidine in Biological Samples, Jesus R. Requena, David L. Price, Suzanne R. Thorpe, and John W. Baynes. Part V. Mitochondrial Mutation and Function with Age. Causes and Consequences of Damage to Mitochondria: Morphological Aspects, Jaime Miquel and Carlo Bertoni-Freddari. Causes and Consequences of Damage to Mitochondria: Study of Functional Aspects by Flow Cytometry, Federico V. Pallardo, Juan Sastre, Jaime Miquel, and José Viña. Analysis of Mitochondrial DNA Mutations: Deletions, Robert W. Taylor, Theresa M. Wardell, Emma L. Blakely, Gillian M. Borthwick, Elizabeth J. Brierley, and Douglass M. Turnbull. Analysis of Mitochondrial DNA Mutations: Point Mutations, Robert W. Taylor, Richard M. Andrews, Patrick F. Chinnery, and Douglass M. Turnbull. Part VI. Immune System and Aging. Assessment of T-Cell Function in the Aged: T-Cell Proliferative and T-Cell Adherence Assays, Ian Beckman. Dendritic Cells in Old Age, Beatrix Grubeck-Loebenstein, Maria Saurwein-Teissl, and Nikolaus Romani. Age-Related Alterations to Natural Killer Cell Function, Erminia Mariani, Corona Alonso, and Rafael Solana. Immunogenetics and Lifespan: HLA, Derek Middleton, Martin D. Curran, and Fionnuala Williams. Part VII. Case Studies. Dietary Restriction and Life-Span Extension, Byung Pal Yu. The Use of Genetically Engineered Mice in Aging Research, Julie K. Andersen. Index.

Editorial Reviews

"Aging Methods and Protocols brings together a wide variety of molecular experimental techniques for studying the aging process. A group of experts related their own aging experimental methods in a step-by-step manner. This format makes it easy to use and adapt specific techniques for individual laboratories. Aging Methods and Protocols contains a unique group of useful experimental methods. The book is highly recommended for academic research-oriented health science libraries. It is also recommended for individual research laboratories investigating the aging process." - E-STREAMS