Alcohol: Methods and Protocols by Laura E. NagyAlcohol: Methods and Protocols by Laura E. Nagy

Alcohol: Methods and Protocols

EditorLaura E. Nagy

Paperback | November 19, 2010

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This book examines the pleiotropic effects of ethanol in animal and cell culture models through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics.
Title:Alcohol: Methods and ProtocolsFormat:PaperbackDimensions:440 pagesPublished:November 19, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617378372

ISBN - 13:9781617378379

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Table of Contents

ContentsPreface Contributors Part I. Animal Models of Acute and Chronic Ethanol ExposureAcute models of ethanol exposure to miceTimothy P. Plackett and Elizabeth J. KovacsA voluntary oral-feeding rat model for pathological alcohol liver injuryGeorge L. Tipoe, Emily C. Liong, Tung-Ming Leung,and Amin A. NanjiIntragastric ethanol infusion model in rodentsHide Tsukamoto, Hasmik Mikrtchyan and Alla DynnykA practical method of chronic ethanol administration in miceRuth A Coleman, Betty M. Young, Lucas E. Turner and Robert T. CookPart II. Animal models of ethanol exposure during developmentAnalysis of ethanol developmental toxicity in ZebrafishRobert L. Tanguay and Mark J. ReimersThe avian embryo in fetal alcohol researchSusan M. SmithArtificial rearingHector D. Dominguez and Jennifer D. ThomasIntragastric intubation of alcohol during the perinatal periodSandra J. Kelly and R. Charles LawrencePart III. Cell culture approaches to studying ethanol exposure9. Human monocytes, macrophages and dendritic cells - alcohol treatment methodsGyongyi Szabo and Pranoti Mandrekar10. Generation and use of primary rat cultures for studies of the effects of ethanolAmanda Lindke, Barbara Tremper-Wells and Michael W. Miller11. Development and properties of HepG2 cells which constitutively express CYP2E1Defeng Wu and Arthur I. Cederbaum12. Modeling the impact of alcohol on cortical development in a dish: Strategies from mapping neural stem cell fateRajesh C. Miranda, Daniel R. Santillano, Cynthia Camarillo, and Douglas DohrmanPart IV. Regulation of Specific Organ Systems in Response to Ethanol13. Acetaldehyde-induced barrier disruption and paracellular permeability in Caco-2 cell monolayerR.K. Rao14. Alcohol-induced oxidative stress in the liver: in vivo measurementsGavin E. Arteel15. Isolation of Kupffer cells from rats fed chronic ethanolMegan R. McMullen, Michele T. Pritchard and Laura E. Nagy 16. Dendritic cells in chronic in vivo ethanol exposure modelsKevin L. Legge and Annette J. Schleuter17. Formation and immunological properties of aldehyde-derived protein adducts following alcohol consumptionGeoffrey M. Thiel, Lynell W. Klassen and Dean J. Tuma18. Assessment of natural killer (NK) and NKT cells in murine spleens and liversMichael R. Shey and Zuhair K. Ballas19. Polyclonal and antigen-specific responses of T cells and T cell subsetsBetty M. Young, Susan Wiechert, Ruth A. Coleman, Prajwal Gurung, and Robert T. Cook20. B Cell studies and chronic ethanol miceShilpi Verma, Carla-Maria A. Alexander, Michael J. Carlson, Lorraine T. Tygrett and Thomas J. Waldschmidt21. Histological analysis of boneUrszula T. Iwaniec, Thomas J. Wronski and Russell T. Turner22. Assessing effects of alcohol consumption on protein synthesis in striated musclesThomas C. Vary and Charles H. Lang23. Methods to investigate the effects of chronic ethanol on adipocytesBecky M. Sebastian, Li Kang, Xiaocong Chen, and Laura E. NagyPart V. Proteomic and Genomic Approaches to Study Responses to Ethanol 24. Proteomic approaches to identify and characterize alterations to the mitochondrial proteome in alcohol liver diseaseShannon M. Bailey, Kelly K. Andrina, Aimee Landar, and Victor M. Darley-Usmar25. Alcoholic liver disease and the mitochondrial ribosome: methods of analysisAlan Cahill and Peter Sykora26. Microarray analysis of ethanol-induced changes in gene expressionRobnet T. Kerns and Michael Miles