Allergy Methods and Protocols by Meinir G. JonesAllergy Methods and Protocols by Meinir G. Jones

Allergy Methods and Protocols

byMeinir G. JonesEditorPenny Lympany

Paperback | November 9, 2010

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Allergy: Methods and Protocols aims to assist the researcher in gaining insight into the molecular mechanisms involved in allergy by featuring an array of protocols. The protocols cover a range of disciplines including allergy, immunology, cell biology, and histology, and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, and T regulatory cells.
Title:Allergy Methods and ProtocolsFormat:PaperbackDimensions:352 pages, 22.9 × 15.2 × 0.17 inPublished:November 9, 2010Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372439

ISBN - 13:9781617372438

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Table of Contents

List of chapters1. Understanding the molecular mechanisms of allergy - Meinir Jones2. T cell - primary culture from peripheral blood - MonikaRaulf-Heimsoth3. Production of T cell lines - Helga Kahlert4. Production of human T cell clones - Adrienne Verhoef5. Mapping of human T-cell epitopes of allergens - Thomas Zeiler andTuomas Virtanen6. Determining MHC restriction of T cell response - Mark Larche7. Short term culture of CD8 cells and intracellular cytokine staining- Beejal Vyas and Alistair Noble8. Isolation, flow cytometric analysis and suppression assay ofCD4+CD25+ T regulatory cells - Hayley Jeal9. Monocyte-derived dendritic cells as antigen presenting cells in Tcell proliferation and cytokine production - Sun-sang J Sung10. Ultrasensitive ELISA for measurement of human cytokine responses inprimary culture - William P. Stefura, J. Darren Campbell, RenéeDouville, Monique J. Stinson, F. Estelle Simons, Allan B. Becker, andKent T. HayGlass11. Quantification of human chemokine production in TLR-stimulated andantigen-specific recall responses - Monique Stinson, Renee Douville,Yuriy Lissitsyn, Melanie Blanchard, William Stefura, Estelle Simons,Allan Becker, Peter Nickerson, Kevin Coombs, Kent HayGlass12. Standardisation of allergen extracts - Jørgen Nedergaard Larsen andSten Dreborg13. Immuno-electrophoresis for the characterisation of allergenextracts - Gitte Nordskov Hansen and Jørgen Nedergaard Larsen14. Conjugation of haptens - Ranulfo Lemus and Meryl H. Karol15. Monoclonal antibodies - Helga Kahlert and Oliver Cromwell16. Purification of antibodies - Per H Larsson and Anne Renström17. Collection of air samples to quantify exposure to airborneallergens - Susan Gordon18. Assay of air sample eluates - Anne Renström and Susan Gordon19. The halogen assay - a new technique for measuring airborne allergen- Euan Tovey, Sandra De Lucca, Leanne Poulos and Tim O'Meara20. Measurement of specific IgG anti-Fel d 1 antibodies - Meinir Jones21. The facilitated-antigen binding (FAB) assay - a protocol to measureallergen-specific inhibitory activity - James N Francis22. Microscopic identification and purity determination of pollengrains - Magdalena Rahl23. Biopanning for the characterisation of allergen mimotopes -Isabella Schöll and Erika Jensen-Jarolim24. Identification of mast cells and mast cell subpopulations - MarkBuckley and Andrew F Walls25. Purification and characterisation of mast cell tryptase and chymase- Alan R McEuen and Andrew F Walls26. Experimental activation of mast cells and their pharmacologicalmodulation - Shaoheng He and Andrew F Walls27. In situ hybridisation - Kayhan T. Nouri-Aria

Editorial Reviews

From the reviews:"The purpose is to provide a comprehensive and readily accessible guide to techniques used in the investigation of allergic diseases. ... Students embarking on an investigative career in allergy and immunology will find this book particularly useful. ... would find a ready place in most laboratories where postgraduate students are working. ... It can be highly recommended to researchers who are starting out, and to those who would like an authoritative and succinct background to their work." (Ronald S. Walls, Doody's Review Service, October, 2008)