Analyzing T Cell Responses: How to analyze cellular immune responses against tumor associated antigens by Dirk NagorsenAnalyzing T Cell Responses: How to analyze cellular immune responses against tumor associated antigens by Dirk Nagorsen

Analyzing T Cell Responses: How to analyze cellular immune responses against tumor associated…

EditorDirk Nagorsen, Francesco M. Marincola

Paperback | October 19, 2010

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This book reviews, describes, categorizes and provides a critical assessment of all assays that have been used for the monitoring of antigen-specific immune responses. In particular, it emphasizes a global approach to the analysis of T cell mediated target/host interactions at the systemic and the peripheral level when such interactions are supposed to occur. The book, therefore, targets a specialized audience of clinical investigators interested in the strategic planning of monitoring aspects of clinical protocols. In addition, the tools described here could be utilized for the search of surrogate biomarkers predictive of treatment responsiveness and/or clinical outcome that are of interest to the biotechnology industry. Biomarker discovery, validation and utilization for class prediction can only be rationally achieved through a comprehensive review of available technologies and the understanding of their unique advantages and limitations. In detail, this book provides an overview of antigen-specific immune biology in human models of tumor and viral disease, discusses modulation of such responses through immune escape and presents cellular assays (cytoxicity, proliferation, cytokine production using ELISPOT, intracellular staining or cytometric assessment, detection of antigen-specific T cells with tetrameric HLA/epitope complexes or MHC-IG dimers, T cell receptor analysis, assessment of T cell receptor/HLA interactions using peptide/HLA-Green Fluorescent Protein complexes incorporation) and molecular assays including quantitative real-time PCR and gene profiling for evaluation of systemic and peripheral immune responses. All chapters were prepared by individuals who pioneered the utilization of such assays and, therefore, provide an in depth overview of the potential of each individual assay.
Title:Analyzing T Cell Responses: How to analyze cellular immune responses against tumor associated…Format:PaperbackDimensions:325 pagesPublished:October 19, 2010Publisher:Springer NetherlandsLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:9048169119

ISBN - 13:9789048169115

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Table of Contents

Chapter 1: Monitoring Antigen-Specific T Cell Responses; Dirk Nagorsen, Francesco M. MarincolaChapter 2: Tumor Associated Antigens; Paul F. RobbinsChapter 3: Immune Escape, Tumor induced immune suppression and immune escape: Mechanisms and Possible Solutions; Theresa L. Whiteside, Michael Campoli, Soldano FerroneChapter 4: Virus Specific T-Cell Responses; Victor AppayChapter 5: Cytotoxicity Assays Killer Lymphocytes in Cancer; Gideon Berke & William R. ClarkChapter 6: Monitoring T Cell Proliferation; Philip D. Hodgkin, Edwin D. Hawkins, Jhaguaral Hasbold, Amanda V. Gett, Elissa K. Deenick, Hilary F. Todd & Mirja HommelChapter 7: Elispot Assay, Assessment of Cellular Immune Responses to Anti-Cancer Vaccines; Theresa L. WhitesideChapter 8: Modified Elispot, Modifications of the Elispot Assay for T Cell Monitoring in Cancer Vaccine Trials; Anatoli MalyguineChapter 9: Intracellular Cytokine Staining, Cytokine flow cytometry for characterization of tumor-specific T cell responses; Carmen Scheibenbogen, Anne Letsch, Anne Marie Asemissen, Alexander Schmittel, Eckhard Thiel & Ulrich KeilholzChapter 10: Cytometric Cytokine Secretion Assay, Detection and Isolation of Antigen-Specific T Cells; Mario AssenmacherChapter 11: Peptide/MHC Tetramer Analysis; Peter P. Lee & Francesco M. MarincolaChapter 12: In Situ MHC Tetramer Staining, In Situ Tetramers; Pamela J. SkinnerChapter 13: MHC-IG Dimeric Molecules, Dimers - MHC-Ig dimeric molecules for the analysis of antigen-specific T cell responses; Tim F. Greten & Firouzeh KorangyChapter 14: TCR Analyses, T-cell receptor CDR3 analysis: Molecular fingerprinting of the T-cell receptor repertoire; Markus J. MaeurerChapter 15: Peptide/HLA-GFP Complexes, Detection of Antigen-Specific T Cells by Acquisition of Peptide/Hla-Gfp Complexes; Utano Tomaru, Yoshihisa Yamano & Steven JacobsonChapter 16: QRT-PCR, Quantitative RT-PCR for the Analysis of T cell Responses in Immunized Cancer Patients; Udai S. KammulaChapter 17: Microarrays, Gene expression profiling approaches for the monitoring of anti-cancer immune responses; Ena WangConcluding Remarks; Dirk Nagorsen & Francesco M. Marincola

Editorial Reviews

From the reviews of the first edition:"This book represents the first comprehensive description, and evaluation of the most important assays utilized to monitor immune responses against tumor associated antigens. . Specifically tailored to the needs of clinical researchers, basic science investigators, and biotechnology innovators, this book provides information that is both sufficiently detailed and eloquently succinct. All discussions are substantiated with a comprehensive literature review. . Analyzing T Cell Responses provides the most substantial and extensive review of immune response-monitoring techniques available to date." (Melanie Hayden, Stephanie Schroter and Boris Minev, Angiogenesis, Vol. 9, 2006)