Angiotensin Protocols by Donna H. WangAngiotensin Protocols by Donna H. Wang

Angiotensin Protocols

EditorDonna H. Wang

Paperback | December 12, 2011

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Donna Wang and a panel of hands-on experts describe in step-by-step detail all the major molecular, biochemical, and functional techniques now used in probing the renin-angiotensin system. These range from the use of embryonic gene targeting to create of mutant strains, to the functional investigation of components of the renin-angiotensin system. Comprehensive and highly practical, Angiotensin Protocols translates expert knowledge into readily reproducible methods that enable all biomedical investigators successfully to explore the role of angiotensin in health and disease today.
Title:Angiotensin ProtocolsFormat:PaperbackDimensions:544 pagesPublished:December 12, 2011Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617371769

ISBN - 13:9781617371769

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Table of Contents

Part I. Introduction to the Renin-Angiotensin SystemHistorical Perspective of the Renin-Angiotensin SystemJohn E. HallThree Angiotensin Paradigms in One Patient: Etiology of Hypertension, Glomerular Hemodynamics, and Long-Term Glomerular ProtectionCheryl L. Laffer and Fernando ElijovichPart II. Transgenic and Gene Targeting MethodsProduction and Use of Chimeric MiceJunji Takaya, Taiji Matsusaka, and Iekuni IchikawaGenetic Manipulation of the Renin-Angiotensin System Using Cre-loxP-RecombinaseCurt D. Sigmund and David E. StecSystemic Delivery of a Transgene in Intact Animals by a Retroviral VectorMichael J. Katovich, Hong-Wei Wang, Craig H. Gelband, and Mohan K. RaizadaAntisense Inhibition of the Renin-Angiotensin SystemDagmara Mohuczy and M. Ian PhillipsPart III. Analysis of Transcriptional and Translational MechanismsAnalysis of Transcriptional Control Mechanisms I: Techniques for Characterization of cis-Regulatory ElementsChristopher T. Sherman and Allan R. BrasierAnalysis of Transcriptional Control Mechanisms II: Techniques for Characterization of trans-Acting FactorsAllan R. Brasier, Christopher T. Sherman, and Mohammad JamaluddinAnalysis of Cytosolic Proteins that Bind to the 5' Leader Sequence of the Angiotensin AT1 Receptor by RNA Electromobility Shift AssayZheng Wu, Kamakshi Krishnamurthi, Koby Mok, and Kathryn SandbergIn Vitro Translation of the Angiotensin AT1 Receptors in Wheat-Germ ExtractsHong Ji, Kamakshi Krishnamurthi, Zheng Wu, and Kathryn SandbergPart IV. Assays for Determination of mRNA LevelsQuantitation of Angiotensin II Receptors by Competitive Reverse-Transcription Polymerase Chain ReactionWei Wu, Aqing Yao, and Donna H. WangAnalysis of RNA by Northern-Blot HybridizationPatricia E. Gallagher and Debra I. DizUse of the Ribonuclease Protection Assay for the Analysis and Characterization of Target mRNAsScott C. Supowit and Donald J. DiPetteIn Situ HybridizationSusan Riggs Runge, Zhaoyong Hu, and Marschall RungePart V. Assays for Determination of Protein Contents and Enzyme ActivityImmunohistochemical Detection of Angiotensin II ReceptorZhi-Qin Wang and Robert M. CareyWestern BlottingNageswara R. Madamanchi and Marschall S. RungeQuantification of Angiotensin-Converting Enzyme (ACE) ActivityQing Cheng Meng and Kathleen H. BerecekQuantification of Angiotensin PeptidesQing Cheng Meng and Kathleen H. BerecekRadiolabeling of Angiotensin PeptidesRobert C. Speth and Joseph W. HardingAssays for Radiolabel-Photoaffinity Labeling of Angiotensin ReceptorsJacqueline Pérodin, Antony A. Boucard, Richard Leduc, Emanuel Escher, and Gaétan GuillemetteRadioligand Binding AssayGaétan Thibault and Ernesto L. SchiffrinAutoradiographic Localization and Quantification of Components of the Renin-Angiotensin System in TissuesSiew Yeen Chai, Andrew M. Allen, Jialong Zhuo, Ingrid Moeller, and Frederick A. O. MendelsohnPart VI. Biochemical Studies of the Renin-Angiotensin SystemMeasurement of Intracellular Free Calcium Ion Concentration in Vascular Smooth Muscle Cells: Fluorescence Imaging of Cytosolic CalciumRhian M. Touyz and Ernesto L. SchiffrinMeasurement of Phospholipidase D Activation in Vascular Smooth Muscle CellsRhian M. Touyz and Ernesto L. SchiffrinMeasurement of Cyclic AMP and Cyclic GMP in Xenopus Oocytes Stimulated with Angiotensin II and Atrial Natriuretic FactorHong JiMeasurement of Nitric Oxide FormationChristian Thorup and Leon C. MooreMeasuring Renin Secretion from Juxtaglomerular CellsArmin Kurtz and Frank SchwedaPart VII. Functional Studies of the Renin-Angiotensin SystemMeasurement of Regional Blood Flow in the Kidney Using Laser-Doppler FlowmetryRichard J. Roman, David L. Mattson, and Allen W. Cowley, JrMeasurement of Renal Tubular Angiotensin IIJohn D. Imig and L. Gabrial NavarThe In Vitro Blood-Perfused Juxtamedullary Nephron TechniqueEdward W. InschoIn Vitro Proximal Tubule Perfusion PreparationJeffrey L. Garvin and Peter J. HarrisIsolated Resistance Artery PreparationJos P. M. Wesselman, Paul H. Ratz, and Russell L. PrewittMeasurement of Vascular DensityAndrew S. Greene and Mark J. RiederFunctional Studies of the Cerebral CirculationJulian H. LombardIndex

Editorial Reviews

"This is an advanced treatise on the renin-angiotensin system (RAS) and its regulation of the cardiovascular, renal, and neural relationships in health and disease. The introduction is ample to move the biological scientist reader up to adequacy to comprehend a complex patient example. ...The purpose shows a goal along with suitable objectives to move traditional medicine into utilization of the amazing explosion of modern biological techniques in RAS applications. It does so with a compilation of experts that foster a clearer picture of the modem RAS field. These are indeed worthy objectives, that surpass what individual papers and review articles can accomplish. Indeed it is needed and it serves as a tremendous thinking stimulator....The introduction by John E. Hall sets the tone and makes this book happen. The genetic manipulation of the RAS by Sigmund and Stec keeps the happening alive. the quantification of angiotensin II receptors by Wu, Yao and Wang is an outstanding peace of work. The cyclic AMP and GMP by Ji was helpful as was the NO from assay work of Thorup and Moore. The closing article on cerebral circulation by Lombard carries the exciting nature of the book to the interested student."-Doody's Health Sciences Book Review Journal"This book with great attention to the recent developments in molecular genetic, biochemical and fysiological techniques can be recommended to all researchers involved in the fundemental research of the RAS at the cellular level." - Acta Cardiologica