Animal Cell Culture: A Practical Approach

Paperback | July 15, 2000

EditorJohn Masters

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This new edition of Animal Cell Culture covers new or updated chapters on cell authentication, serum-free culture, apoptosis assays, FISH, genetic modification, scale-up, stem cell assays, 3-dimensional culture, tissue engineering and cytotoxicity assays. Detailed protocols for a wide varietyof methods provide the core of each chapter, making new methodology easily accessible. Everyone working in biological and medical research, whether in academia or a commercial organization, practising cell culture will benefit greatly from this book.

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This new edition of Animal Cell Culture covers new or updated chapters on cell authentication, serum-free culture, apoptosis assays, FISH, genetic modification, scale-up, stem cell assays, 3-dimensional culture, tissue engineering and cytotoxicity assays. Detailed protocols for a wide varietyof methods provide the core of each chapter...

John Masters is at University College London.

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Format:PaperbackPublished:July 15, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199637962

ISBN - 13:9780199637966

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Table of Contents

Introduction to basic principlesScaling-up of cell culturesCell line preservation and characterizationDevelopment of serum-free mediaThree dimension cultureTissue engineeringCytotoxicity and viability assaysFluorescence in situ hybridization (FISH)Genetic modificationEpithelial stem cell identification, isolation and cultureApoptosis assays

Editorial Reviews

"ÝA¨ number of experienced cell biologists . . . collectively have done a fine job. . . . ÝI¨t will help many people in cell culture, . . . especially where postgraduates and postdoctoral students want some quick clear guidance."--Cell Biology International