Antibody Engineering: Methods and Protocols, Second Edition by Patrick ChamesAntibody Engineering: Methods and Protocols, Second Edition by Patrick Chames

Antibody Engineering: Methods and Protocols, Second Edition

EditorPatrick Chames

Hardcover | August 21, 2012

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More than ever, antibodies are being recognized as a major drug modality in a variety of diseases, including cancer, autoimmune diseases, infectious diseases, or even neurodegenerative disorders. Over 30 therapeutic antibodies have been approved and novel molecules are entering clinical trials at an average rate of 50 per year and that is predicted to continue well into the future. Notwithstanding the many achievements already made in the field, there is still a lot of room for improvements for these molecules in terms of activity, and a plethora of approaches have been attempted to optimize these molecules. Antibody Engineering: Methods and Protocols, Second Edition was compiled to give complete and easy access to a variety of antibody engineering techniques, starting from the creation of antibody repertoires and efficient ways to select binders from these repertoires, to their production in various hosts, their detailed characterization using various well established techniques, and to the modification and optimization of these lead molecules in terms of binding activity, specificity, size, shape, and more. Written in the successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Antibody Engineering: Methods and Protocols, Second Edition serves as an invaluable resource for both experts and those new to the field, and most of all as a source of inspiration for the creation of the antibodies of tomorrow.
Title:Antibody Engineering: Methods and Protocols, Second EditionFormat:HardcoverDimensions:685 pages, 10 × 7.01 × 0.1 inPublished:August 21, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617799734

ISBN - 13:9781617799730

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Table of Contents

Part I. In silico Methods 1. Use of IMGT® Databases and Tools for Antibody Engineering and HumanizationMarie-Paule Lefranc, François Ehrenmann, Chantal Ginestoux, Véronique Giudicelli, and Patrice Duroux 2. Computer-Assisted Modeling of Antibody Variable DomainsOscar H. P. Ramos  Part II. Generation of Diversity 3. Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma CellsLars Toleikis and André Frenzel 4. Human in Cell scFv Library from Infiltrating B CellSylvie Peraldi-Roux                                    5. Construction of Human Naive Antibody Gene LibrariesMichael Hust, André Frenzel, Torsten Meyer, Thomas Schirrmann, and Stefan Dübel    6. Synthetic Customized scFv Libraries  Gautier Robin and Pierre Martineau         7. Selection of Stable scFv Antibodies by Phage Display       Eeva-Christine Brockmann        8. Generation of Single Domain Antibody Fragments Derived from Camelids and Generation of Manifold Constructs Cécile Vincke, Carlos Gutiérez, Ulrich Wernery, Nick Devoogdt, Gholamreza Hassanzadeh-Ghassabeh, and Serge Muyldermans 9. Generation and Isolation of Target Specific Single Domain Antibodies from Shark Immune Repertoires Mischa Roland Müller, Ronan O'Dwyer, Marina Kovaleva, Fiona Rudkin, Helen Dooley, and Caroline Jane Barelle 10. Generation of Human Single Domain Antibody Repertoires by Kunkel Mutagenesis Kip Dudgeon, Romain Rouet, Kristoffer Famm, and Daniel Christ  Part IV. Selections of Lead Candidate 11. Phage Display and Selections on Purified AntigensJulie Matz and Patrick Chames     12. Phage Display and Selections on Cells Klervi Even-Desrumeaux and Patrick Chames  13. Humanization by CDR Grafting and Specificity-Determining Residue Grafting  Jin Hong Kim and Hyo Jeong Hong 14. Humanization by Guided Selections Sang Jick Kim and Hyo Jeong Hong             15. Selection of Antibody Fragments by Yeast Display   Nathalie Scholler       16. Evolution of Antibodies in vitro by Ribosome DisplayBryan M. Edwards and Mingyue He               17. Mammalian Cell Display of Full Length IgG        Chen Zhou and Wenyan (David) Shen Part V. Production of Recombinant Antibodies 18. Production of Antibody Fragments in Escherichia coli Tomohisa Katsuda, Hiroyuki Sonoda, and Hideki Yamaji         19. Production of Antibody Derivatives in the Methylotrophic Yeast Pichia pastorisSteve Schoonooghe, Jannick Leoen, and Jurgen Haustraete                  20. Monoclonal Antibody Expression in Mammalian Cells Richard (Yi) Zhang and Wenyan (David) Shen         21. Production of Recombinant Antibodies in Drosophila melanogaster S2 Cells Daniel X. Johansson, Thomas Krey, and Oskar Andersson 22. Production of Antibody Fragments Using the Baculovirus-Insect Cell System Takanori Furuta, Takafumi Ogawa, and Hideki Yamaji                  23. Transient and Stable Expression of Antibodies in Nicotiana SpeciesJ. Christopher Hall, Michael D. McLean and Freydoun Garabagi     Part VI. Variable Domain Optimization 24. Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon ResonanceStephen Hearty, Paul Leonard, and Richard O'Kennedy                25. Affinity Determination of Biotinylated Antibodies by Flow CytometryKlervi Even-Desrumeaux and Patrick Chames        26. Affinity Maturation of Antibodies: Optimized Methods to Generate High Quality scFv Libraries and Isolate IgG Candidates by High Throughput ScreeningLaurence Renaut, Céline Monnet, Olivier Dubreuil, Ouafa Zaki, Fabien Crozet, Khalil Bouayadi, Hakim Kharrat and Philippe Mondon       27. Affinity Maturation by Semi Rational Approaches  Rodrigo Barderas, Johan Desmet, Philippe Alard, and J. Ignacio Casal        28. Molecular Scanning Mutagenesis: Combining Ribosome Display, Random Mutagenesis and Bioinformatic Analysis for Protein Engineering Alfredo Darmanin-Sheehan, William James Jonathan Finlay Orla Cunningham, and Brian Joseph Fennell     Part VII. Fc Engineering 29. Fucose-Targeted Glyco-Engineering of Pharmaceutical Cell LinesChristiane Ogorek, Ingo Jordan, Volker Sandig, and Hans Henning von Horsten  30. Fc engineering: Design, expression and functional characterization of antibody variants with improved effector functionStefanie Derer, Christian Kellner, Sven Berger, Thomas Valerius and Matthias Peipp    31. Fc engineering: Serum half life modulation through FcRn binding Tove Olafsen                           32. Monoclonal Antibody Lead Characterization - In vitro and in vivo methods Axel Hernandez, Julie Parmentier, Youzhen Wang, Jane Cheng and Gadi Gazit Bornstein   Part VIII. Innovative Formats 33. Production, generation and characterization of scFv TNF ligand fusion proteinsAndrea Fick, Agnes Wyzgol and Harald Wajant       34. Antibody-IL-2 fusion proteins for tumor targeting Andreas A. Hombach and Hinrich Abken     35. Recombinant immunotoxins containing low endotoxins for clinical and animal studies Masanori Onda  36. Chimeric antigen receptors for T-cell based therapy Eleanor J. Cheadle, Vicky Sheard, Andreas A. Hombach, Markus Chmielewski, Tobias Riet, Cor Berrevoets, Erik Schooten, Cor Lamers, Hinrich Abken, Reno Debets, David E. Gilham                37. Selection and Use of Intracellular Antibodies (Intrabodies)Sandrine Moutel, Clément Nizak, and Franck Perez       38. Radiolabeled Antibodies for Imaging and Therapy  Jacques Barbet, Manuel Bardiès, Mickael Bourgeois, Jean-François Chatal, Michel Chérel, François Davodeau, Alain Faivre-Chauvet, Jean-François Gestin, and Françoise Kraeber-Bodéré   39. Design and Production of Multimeric Antibody Fragments, Focused on Diabodies with Enhanced Clinical Efficacy Glenn A. Powers, Peter J. Hudson, and Michael P. Wheatcroft                                  40. Production of Bispecific Antibodies: Diabodies and Tandem scFvNora Hornig and Aline Färber-Schwarz