Antibody Methods and Protocols by Gabriele ProetzelAntibody Methods and Protocols by Gabriele Proetzel

Antibody Methods and Protocols

EditorGabriele Proetzel, Hilmar Ebersbach

Hardcover | June 23, 2012

Pricing and Purchase Info

$192.95 online 
$219.95 list price save 12%
Earn 965 plum® points

In stock online

Ships free on orders over $25

Not available in stores

about

The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies.  Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments.  The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments.  Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Antibody Methods and Protocols provides a broad and useful background to support ongoing efforts by novices and experts alike and encourages the development of new imaginative approaches to this vital area of study.
Title:Antibody Methods and ProtocolsFormat:HardcoverDimensions:335 pages, 10 × 7.01 × 0.01 inPublished:June 23, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617799300

ISBN - 13:9781617799303

Look for similar items by category:

Customer Reviews of Antibody Methods and Protocols

Reviews

Table of Contents

1. Antigen Presentation for the Generation of Binding Molecules            Hilmar Ebersbach, Gabriele Proetzel, and Chonghui Zhang 2. Recombinant Antibodies and In Vitro Selection Technologies            C. Ronald Geyer, John McCafferty, Stefan Dübel, Andrew R.M. Bradbury, and Sachdev S. Sidhu 3. Phage Display            Konstantin Petropoulos 4. Generation of Human Fab Libraries for Phage Display            Christoph Rader 5. Selection of Human Fab Libraries by Phage Display            Christoph Rader 6. Ribosome Display            George Thom and Maria Groves 7. Hybridoma Technology for the Generation of Monoclonal Antibodies            Chonghui Zhang 8. The Application of Transgenic Mice for Therapeutic Antibody Discovery            E-Chiang Lee and Michael Owen 9. Production of Human or Humanized Antibodies in Mice            Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogné 10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies            Elisabetta Traggiai 11. Kinetic Screening in the Antibody Development Process            Michael Schräml and Matthias Biehl 12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection            Michael Schräml and Leopold von Proff 13. Determination of Antibody Glycosylation by Mass Spectrometry            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll 14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format            Jiahui Yang and Christoph Rader 15. PEGylation of Antibody Fragments for Half-Life Extension            Simona Jevsevar, Mateja Kusterle, and Maja Kenig 16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann 17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function            Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies            Virginie Pascal, Brice Laffleur, and Michel Cogné            Konstantin Petropoulos 4. Generation of Human Fab Libraries for Phage Display            Christoph Rader 5. Selection of Human Fab Libraries by Phage Display            Christoph Rader 6. Ribosome Display            George Thom and Maria Groves 7. Hybridoma Technology for the Generation of Monoclonal Antibodies            Chonghui Zhang 8. The Application of Transgenic Mice for Therapeutic Antibody Discovery            E-Chiang Lee and Michael Owen 9. Production of Human or Humanized Antibodies in Mice            Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogné 10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies            Elisabetta Traggiai 11. Kinetic Screening in the Antibody Development Process            Michael Schräml and Matthias Biehl 12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection            Michael Schräml and Leopold von Proff 13. Determination of Antibody Glycosylation by Mass Spectrometry            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll 14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format            Jiahui Yang and Christoph Rader 15. PEGylation of Antibody Fragments for Half-Life Extension            Simona Jevsevar, Mateja Kusterle, and Maja Kenig 16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann 17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function            Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies            Virginie Pascal, Brice Laffleur, and Michel Cogné            Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogné 10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies            Elisabetta Traggiai 11. Kinetic Screening in the Antibody Development Process            Michael Schräml and Matthias Biehl 12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection            Michael Schräml and Leopold von Proff 13. Determination of Antibody Glycosylation by Mass Spectrometry            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll 14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format            Jiahui Yang and Christoph Rader 15. PEGylation of Antibody Fragments for Half-Life Extension            Simona Jevsevar, Mateja Kusterle, and Maja Kenig 16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann 17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function            Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies            Virginie Pascal, Brice Laffleur, and Michel Cogné            Elisabetta Traggiai 11. Kinetic Screening in the Antibody Development Process            Michael Schräml and Matthias Biehl 12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection            Michael Schräml and Leopold von Proff 13. Determination of Antibody Glycosylation by Mass Spectrometry            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll 14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format            Jiahui Yang and Christoph Rader 15. PEGylation of Antibody Fragments for Half-Life Extension            Simona Jevsevar, Mateja Kusterle, and Maja Kenig 16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann 17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function            Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies            Virginie Pascal, Brice Laffleur, and Michel Cogné            Michael Schräml and Matthias Biehl 12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection            Michael Schräml and Leopold von Proff 13. Determination of Antibody Glycosylation by Mass Spectrometry            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll 14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format            Jiahui Yang and Christoph Rader 15. PEGylation of Antibody Fragments for Half-Life Extension            Simona Jevsevar, Mateja Kusterle, and Maja Kenig 16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann 17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function            Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies            Virginie Pascal, Brice Laffleur, and Michel Cogné            Michael Schräml and Leopold von Proff 13. Determination of Antibody Glycosylation by Mass Spectrometry            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll 14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format            Jiahui Yang and Christoph Rader 15. PEGylation of Antibody Fragments for Half-Life Extension            Simona Jevsevar, Mateja Kusterle, and Maja Kenig 16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann 17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function            Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies            Virginie Pascal, Brice Laffleur, and Michel Cogné 13. Determination of Antibody Glycosylation by Mass Spectrometry            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll 14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format            Jiahui Yang and Christoph Rader 15. PEGylation of Antibody Fragments for Half-Life Extension            Simona Jevsevar, Mateja Kusterle, and Maja Kenig 16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann 17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function            Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies            Virginie Pascal, Brice Laffleur, and Michel Cogné            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll 14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format            Jiahui Yang and Christoph Rader 15. PEGylation of Antibody Fragments for Half-Life Extension            Simona Jevsevar, Mateja Kusterle, and Maja Kenig 16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann 17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function            Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies            Virginie Pascal, Brice Laffleur, and Michel Cogné            Jiahui Yang and Christoph Rader 15. PEGylation of Antibody Fragments for Half-Life Extension            Simona Jevsevar, Mateja Kusterle, and Maja Kenig 16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann 17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function            Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies            Virginie Pascal, Brice Laffleur, and Michel Cogné            Simona Jevsevar, Mateja Kusterle, and Maja Kenig 16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann 17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function            Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies            Virginie Pascal, Brice Laffleur, and Michel Cogné            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann 17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function            Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies            Virginie Pascal, Brice Laffleur, and Michel Cogné            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann 18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function            Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies            Virginie Pascal, Brice Laffleur, and Michel Cogné            Robert F. Kelley and Y. Gloria Meng 19. Class-Specific Effector Functions of Therapeutic Antibodies            Virginie Pascal, Brice Laffleur, and Michel Cogné            Virginie Pascal, Brice Laffleur, and Michel Cogné