Antisense Technologies: A Practical Approach

Paperback | September 1, 1997

EditorConrad Lichtenstein, Wolfgang Nellen

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Antisense technology is a powerful procedure that permits the controlled silencing of a specific gene for investigations of mRNA and protein function. This valuable text provides proven step-by-step protocols for antisense techniques in a range of different organisms and cell culture systems.In addition it discusses the potential benefits and problems for various antisense methods which complement gene knock-out experiments. The book includes antisense techniques such as: analysis of nucleic acid structures; measurement and evaluation of antisense effects; selection, preparation andthe use of antisense oligonucleotides; in vitro RNA transcription; construction strategies, testing and optimization of catalytic antisense RNAs based on hammerhead ribozymes; synthesis and evaluation of 2-5 A- antisense chimeras for targeted degradation of RNA. The application of these technologiesare then described, with chapters on antisense techniques in IDictyostelium, plants and the medical uses and benefits of antisense sense technology both in vitro and in vivo. Finally, non-antisense effects of oligonucleotides, "anti-sense rescue", shotgun antisense mutagenesis, and in vivo, in vitroand in situ detection of sense:antisense duplexes by structure-specific anti-RNA antibodies are discussed. The numerous hints and tips for success and advice on trouble shooting, make this authoritative text invaluable to all those researchers who work on gene expression, RNA transcription, orprotein function.

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Antisense technology is a powerful procedure that permits the controlled silencing of a specific gene for investigations of mRNA and protein function. This valuable text provides proven step-by-step protocols for antisense techniques in a range of different organisms and cell culture systems.In addition it discusses the potential benef...

Conrad Lichtenstein is at Queen Mary and Westfield College, London. Wolfgang Nellen is at Universitat Kassel, Kassel.
Format:PaperbackDimensions:326 pages, 9.21 × 6.14 × 0.75 inPublished:September 1, 1997Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199635838

ISBN - 13:9780199635832

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Table of Contents

1. Nucleic acid structures2. Evaluation of antisense effects3. Selecting, preparing, and handling antisense oligodeoxyribonucleotides4. In vitro RNAs5. Catalytic antisense RNA based on hammerhead ribozymes6. 2-5 A-Antisense chimaeras for targeted degradation of RNA7. In vitro applications of antisense RNA and DNA8. Antisense applications in Dictyostelium: a lower eukaryotic model system9. Applications of antisense technology in plants10. Antisense molecules and ribozymes: medical applications11. Non-antisense effects of oligodeoxynucleotides12. Antisense rescue13. Shotgun antisense mutants14. Detection of sense-antisense duplexes by structure-specific anti-RNA antibodies

Editorial Reviews

`'...this volume reaches the standard we have come to expect from the Practical Approach series. The standard of presentation reflects the expertise of the authors and the protocol are described clearly with useful practical notes.''Glenn Matthews, SGM Quarterly Nov. 1998.