Antiviral RNAi: Concepts, Methods, and Applications by Ronald P. van RijAntiviral RNAi: Concepts, Methods, and Applications by Ronald P. van Rij

Antiviral RNAi: Concepts, Methods, and Applications

EditorRonald P. van Rij

Hardcover | March 8, 2011

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Viruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs - of viral or cellular origin - affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi. Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. Divided into five convenient parts, this detailed volume reviews important basic concepts in the field of antiviral RNAi, provides experimental and bio-informatic tools for the analysis of small silencing RNAs, covers methods to biochemically dissect RNAi-based antiviral defense and viral counter-defense mechanisms, describes methods for the design, expression, and delivery of therapeutic antiviral siRNAs, and finally presents genome-wide RNAi approaches for the identification of factors involved in virus replication. Written in the highly successful Methods in Molecular BiologyT series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology.
Title:Antiviral RNAi: Concepts, Methods, and ApplicationsFormat:HardcoverDimensions:422 pagesPublished:March 8, 2011Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617790362

ISBN - 13:9781617790362

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Table of Contents

Part I: General Introduction                                     1. Defense and counter-defense in the RNAi-based antiviral immune system in insects            Joël T. van Mierlo, Koen W.R. van Cleef, and Ronald P. van Rij                              2. RNAi and cellular miRNAs in infections by mammalian virusesJoost Haasnoot and Ben Berkhout                              3. Viral miRNAs        Karlie Plaisance-Bonstaff, and Rolf Renne                             4. Progress in RNAi-based antiviral therapeutics  Jiehua Zhou and John J. Rossi                                     5. Chemical modification of small interfering RNA          Jesper B. Bramsen and Jørgen Kjems                                      Part II: Viral small RNAs 6. Viral small RNA cloning and sequencing           Valérie Gausson and Maria-Carla Saleh                                 7. Visitor, an informatic pipeline for analysis of viral siRNA sequencing datasets         Christophe Antoniewski                                  8. Computational prediction of viral miRNAs       Adam Grundhoff                                 9. Detection of Viral microRNAs by Northern Blot Analysis       Lydia V. McClure, Yao-Tang Lin, and Christopher S. Sullivan                                 10. Detection of viral microRNA with S1 Nuclease Protection assay       Matthias John and Sébastien Pfeffer                           11. Characterization of RISC associated adenoviral small RNAs            Ning Xu and Göran Akusjärvi                                                                                    Part III: RNAi-based antiviral defense                                                 12. Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell CultureKoen W.R. van Cleef, Joël T van Mierlo, Marius van den Beek, and Ronald P. van Rij                              13. Dicer assay in Drosophila S2 cell extract          Baojun Yang and Hongwei Li                                    14. Slicer activity in Drosophila melanogaster S2 extract  Arabinda Nayak and Raul Andino                             15. Gel Mobility Shift Assays for RNA-binding viral RNAi suppressors            Tibor Csorba and József Burgyán                               16. dsRNA uptake in adult Drosophila       Benjamin Obadia and Maria-Carla Saleh                                                                                 Part IV: Antiviral RNAi therapy                                                17. Design of small interfering RNAs for antiviral applications   Diana Rothe, Erik J. Wade, and Jens Kurreck                                    18. RNAi inducing lentiviral vectors for anti-HIV-1 gene therapy           Ying Poi Liu, Jan-Tinus Westerink, Olivier ter Brake, and Ben Berkhout                             19. Production of multicopy shRNA lentiviral vectors for antiviral therapy      Scot D. Henry, Quiwei Pan, and Luc J.W. van der Laan                               20. Intranasal delivery of antiviral siRNA  Sailen Barik                              21. Antibody mediated delivery of siRNAs for anti-HIV therapy            Sang-Soo Kim, Sandesh Subramanya, Dan Peer, Motomu Shimaoka, and Premlata Shankar                                  22. Aptamer-targeted RNAi for HIV-1 therapy    Jiehua Zhou and John J. Rossi                                                                                                  Part V: RNAi screens to study virus-host interactions                                                23. RNAi screening for host factors involved in viral infection using Drosophila cells  Sara Cherry                              24. Genome-wide RNAi Screen for Viral Replication in Mammalian Cell Culture       Bhupesh K. Prusty, Alexander Karlas, Thomas F. Meyer, and Thomas Rudel                                  25. RNAi screening in mammalian cells to identify novel host cell molecules involved in the regulation of viral infections        Carolyn B. Coyne and Sara Cherry

Editorial Reviews

From the reviews:"This well-illustrated book details the necessary protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as the development and optimization of RNAi-based antiviral drugs. The book is intended for both novice and experienced researchers . . It also will be of immense help to graduate students, physicians who treat patients with infectious diseases, and other researchers interested in the field of antiviral RNAi. . a well-illustrated and authoritative resource on important basic concepts in the field of antiviral RNAi." (Omer Iqbal, Doody's Review Service, July, 2011)