Apoptosis and Cancer: Methods and Protocols by Gil MorApoptosis and Cancer: Methods and Protocols by Gil Mor

Apoptosis and Cancer: Methods and Protocols

EditorGil Mor

Paperback | November 19, 2010

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The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing the mechanisms of apoptosis, as well as the industry laboratory, aimed at identifying new target molecules or screening for new compounds with potential clinical use.
Title:Apoptosis and Cancer: Methods and ProtocolsFormat:PaperbackDimensions:277 pages, 9.02 × 5.98 × 0 inPublished:November 19, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617375829

ISBN - 13:9781617375828

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Table of Contents

APOPTOSIS AND CANCER Methods and Protocols Methods in Molecular Medicine Editors: Gil Mor MD, PhD Department of Obstetrics, Gynecology and Reproductive Science Yale University School of Medicine and Ayesha Alvero MD Department of Obstetrics, Gynecology and Reproductive Science Yale University School of Medicine Table of Contents: Chapter 1 (review) Modulation of Apoptosis to Reverse Chemoresistance Gil Mor, Michele K. Montagna and Ayesha B. Alvero Chapter 2. Caspase-3 Activation is a Critical Determinant of Genotoxic Stress-Induced Apoptosis Suparna Mazumder1, Dragos Plesca1,3, and Alexandru Almasan1,2, Chapter 3. Flow cytometry enumeration of apoptotic cancer cells by apoptotic rate *David Diaz, BSc *Alfredo Prieto BSc, PhD, *, Eduardo Reyes, BSc, PhD, *Hugo Barcenilla, BSc, *Jorge Monserrat, BSc, PhD, *, Melchor Alvarez-Mon, MD, PhD. Chapter 4 Detection of cancer-related proteins in fresh-frozen ovarian cancer samples using Laser capture microdissection Dan-Arin Silasi, Ayesha B. Alvero, Jechiel Mor, Rui Chen, Han-Hsuan Fu, Michele K. Montagna and Gil Mor Chapter 5 Flow cytometric detection of activated caspase-3 Richard Fox1 and Martine Aubert2 Chapter 6 The ChemoFx® Assay: An Ex Vivo Chemo-Sensitivity and Resistance Assay for Predicting Patient Response to Cancer Chemotherapy Stacey L. Brower, Ph.D., Jeffrey E. Fensterer, Jason E. Bush, Ph.D., MBA Chapter 7 Correlation of caspase activity and in vitro chemo-response in epithelial ovarian cancer cell lines Ayesha B. Alvero, Michele K. Montagna, and Gil Mor Chapter 8 Assessing Expression of Apoptotic Markers Using Large Cohort Tissue Microarrays Elah Pick, Mary M. McCarthy and Harriet M. Kluger Chapter 9 Mitochondria potential, Bax "activation" and programmed cell death. C. Michael Knudson and Nicholas M. Brown Chapter 10 In Vitro and In Vivo Apoptosis Detection Using Membrane Permeant, Fluorescent Labeled Inhibitors of Caspases Brian W. Lee*, Michael R. Olin, Gary L. Johnson, and Robert J. Griffin Chapter 11 Caspase Activity Assays Andrew L. Niles, Richard A. Moravec, Terry L. Riss Chapter 12 Multiplex Caspase Activity and Cytotoxicity Assays Andrew L. Niles, Richard A. Moravec, Terry L. Riss Chapter 13 Homogeneous, Bioluminescent Proteasome Assays Martha A. O'Brien, Richard A. Moravec, Terry Riss, and Robert F. Bulleit Chapter 14 A simple method for profiling miRNA expression Jia-Wang Wang, PhD*, Jin Q. Cheng, Ph.D., M.D*. Chapter 15 Apoptotic Caspase Activation and Activity Jean-Berard Denault and Guy S. Salvesen Chapter 16 Biochemical Analysis of the native TRAIL Death-inducing Signalling Complex (DISC) Henning Walczak and Tobias L. Haas Chapter 17 Laser Microdissection Sample Preparation for RNA Analyses Christopher J. Vega

Editorial Reviews

From the reviews:"Divided among 17 chapters, the condensed information about apoptosis, its pathways and the performance of progressive techniques for apoptosis assessment are presented. . The valuable and most appreciated part of each chapter are notes - the reader will find here the plethora of hints, the real grain of wisdom, virtual wizard that saves a lot of struggling time at benchtop. This book belongs to the category of must-have books." (J. Sedlák, Neoplasma, September, 2008)