Apoptosis Techniques and Protocols by Andréa C. LeBlancApoptosis Techniques and Protocols by Andréa C. LeBlanc

Apoptosis Techniques and Protocols

EditorAndréa C. LeBlanc

Paperback | November 9, 2010

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In this revised and expanded second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of pro-apoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays.
Title:Apoptosis Techniques and ProtocolsFormat:PaperbackDimensions:266 pages, 9.02 × 5.98 × 0 inPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617373060

ISBN - 13:9781617373060

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Table of Contents

APOPTOSIS TECHNIQUES AND PROTOCOLS, 2ND EDITIONEdited by:Andréa C. LeBlancCONTENTSPreface to the SeriesPrefaceContributorsMolecular Characterization of the Proapoptotic Protein BaxYi-Te Hsu and Soraya SmailiAssessing Cytochrome-c Release from MitochondriaDouglas W. Ethell and Douglas R. GreenMonitoring Caspases in Neuronal Cell DeathYounes Bounhar, Omar Tounekti, and Andréa C. LeBlancHSV Amplicon Vectors in Neuronal Apoptosis StudiesKathleen A. Maguire-Zeiss, William J. Bowers, and Howard J. FederoffMicroinjections to Study the Specific Role of Proapoptotic Proteins in NeuronsYan Zhang and Andréa C. LeBlancDesigning Microarray Experiments for NeurobiologyP. Scott Eastman and Jeanne F. LoringAnalysis of Gene Expression by Nylon Membrane cDNA ArraysH. Michael Tucker and Steven EstusGlyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic DrugsMark D. Berry and Paula C. AsheAnalytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic NeuronsBarbara Karten, Bronwyn L. MacInnis, Hubert Eng, Yoko Azumaya, Grace Martin, Karen Lund, Russell C. Watts, Jean E. Vance, Dennis E. Vance, and Robert B. CampenotDetection and Analysis of SynaptosisGreg M. Cole and Karen GylysIn Situ Detection of Apoptotic NeuronsKevin A. RothApoptotic and Oxidative Indicators in Alzheimer's DiseaseArun K. Raina, Lawrence M. Sayre, Craig S. Atwood, Catherine A. Rottkamp, Ayala Hochman, Xiongwei Zhu, Mark E. Obrenovich, Shun Shimohama, Akihiko Nunomura, Atsushi Takeda, George Perry, and Mark A. SmithIndex

Editorial Reviews

Review of the first edition:"The book is useful for an actively working neuroscientist who is going to study apotosis and has enough experience to choose the most suitable models and protocols among all the diverse offerings of the book." Three Stars. -Doody's Health Sciences Book Review Journal "...the book has updated many of the state-of-the-art techniques for analysis of neuronal apoptosis. More importantly, the book also offers the first-hand and step-by-step experimental details for molecular, cellular, biochemical and immunohistochemical assays of neuronal apoptotic death." - Biotechnic & Histochemistry