Arabidopsis: A Practical Approach

Paperback | January 6, 2000

EditorZoe Wilson

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Arabidopsis has long been acknowledged as the 'Botanical Drosophila' with its small genome, low levels of repetitive DNA, small size and fast generation time it is an ideal molecular genetic tool for the analysis of development in higher plants. Arabidopsis: A Practical Approach provides anintroduction to most of the key techniques required for the use of Arabidopsis as an experimental system. It gives a basic introduction to the optimal growth conditions and genetic resources available for Arabidopsis, how this material should be handled, maintained and used. Individual chaptersdescribe strategies for the identification, mapping (using multi-marker lines and recombinant inbreds), and characterisation of different mutants by microscopy, molecular cytogenetics and gene expression analysis. Different cloning strategies, using transposons, T-DNA and map position are describedin detail. Sequencing of the Arabidopsis genome will be completed in 2000 and bioinformatics are of key importance; the tools that are available and where they can be found on the Web are presented.

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Arabidopsis has long been acknowledged as the 'Botanical Drosophila' with its small genome, low levels of repetitive DNA, small size and fast generation time it is an ideal molecular genetic tool for the analysis of development in higher plants. Arabidopsis: A Practical Approach provides anintroduction to most of the key techniques re...

Zoe Wilson is at University of Nottingham.

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Format:PaperbackPublished:January 6, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199635641

ISBN - 13:9780199635641

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Table of Contents

Growth, maintenance and use of Arabidopsis genetic resourcesPreservation and handling of stock centre clonesGenetic mapping using recombinant inbred linesArabidopsis mutant characterisation: microscopy, mapping and gene expression analysisClassical and molecular cytogenetics of ArabidopsisTissue culture, transformation and transient gene expression in ArabidopsisTransposon and T-DNA mutagenesisMap based cloning in ArabidopsisYACs, BACs, cosmids and nucleotide sequencesWeb-based bioinformatic tools for Arabidopsis researchers

Editorial Reviews

"... valuable for scientists who are beginning to analyse mutations in Arabidopsis." Journal of plant Physiology