Asthma by K. Fan ChungAsthma by K. Fan Chung

Asthma

EditorK. Fan Chung, Ian Adcock

Paperback | November 9, 2010

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K. Fan Chung and Ian Adcock bring together the first collection of laboratory methods for studying the molecular and cellular mechanisms of asthma. Leading experts describe highly effective methods for obtaining cells from asthmatic airways, analyzing gene and protein expression in these clinical samples, and using molecular and cellular tools for studying cytokine expression and release in this disease. There are also cutting-edge techniques for studying asthma-related genes and genetic polymorphisms, and for understanding the effects of asthma treatment. User-friendly and state-of-the-art, Asthma: Mechanisms and Protocols provides all asthma researchers-whether novice or experienced, whether in experimental or clinical research-with a first-class collection of readily reproducible pharmacological, cellular, molecular, biochemical, and genetic methods for elucidating not only the mechanisms of the disease itself, but also of the drugs for asthma's treatment.
Title:AsthmaFormat:PaperbackDimensions:360 pages, 9.02 × 5.98 × 0 inPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617371033

ISBN - 13:9781617371035

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Table of Contents

Asthma: Application of Cell and Molecular Biology Techniques to Unravel Causes and Pathophysiological Mechanisms, K. Fan Chung and Ian Adcock. Culture of Normal Human Airway Epithelial Cells and Measurement of Mucin Synthesis and Secretion, Reen Wu. Brush Biopsy and Culture of Airway Epithelial Cells: b-Adrenoceptor System Function, Steven G. Kelsen, David Ciccolella, and Kathleen Brennan. Isolation and Characterization of Human Airway Fibroblasts in Culture, Jamila Chakir, Jean Dubé, Michel Laviolette, Francine Goulet, Lucie Germain, François Auger, and Louis-Phillipe Boulet. Flow Cytometric Analysis of Blood Monocytes and Alveolar Macrophages, Marcia L. Taylor and Bruce S. Bochne. Expression of Interleukin-10 and Granulocyte-Macrophage Colony-Stimulating Factor in Blood Monocytes and Alveolar Macrophages, Matthias John and Sam Lim. Mitogen-Activated Protein Kinase Activation in Eosinophils, Patricia M. de Souza, Hannu Kankaaranta, Mark A. Giembycz, and Mark A. Lindsay. Human Eosinophil Isolation and the Measurement of Apoptosis, Hannu Kankaaranta, Patricia M. de Souza, Mark A. Giembycz, and Mark A. Lindsay. Adhesion of T-Cells to Airway Smooth Muscle Cells, Aili L. Lazaar and Reynold A. Panettieri, Jr. Proliferation of Circulating T-Lymphocytes, Mark Larché. Differential Display Analysis of Airway Epithelial Inflammatory Genes, Lisa M. Schwiebert. Analysis of Transcription Factor Activation: NFkB as a Regulator of Inflammatory Genes in Epithelial Cells, Robert Newton and Ian M. Adcock. Transient Transgenic Approaches for Investigating the Role of Granulocyte-Macrophage Colony-Stimulating Factor in Pulmonary Inflammation and Immune Diseases, Zhou Xing, Martin Stämpfli, and Jack Gauldie. Quantitative Analysis of RANTES mRNA in Endobronchial Biopsies Using Polymerase Chain Reaction, Neville Berkman. Intracellular Cytokine Staining for Analysis by Flow Cytometry, Anthony J. Frew, Jacqueline Madden, and Petros Bakakos. Localization of IL-4 and IL-5 mRNA by In Situ Hybridization in Bronchial Biopsies, Yutaka Nakamura, Pota Christodoulopoulos, and Qutayba Hamid. Immunohistochemical Analysis of Adhesion Molecules in Airway Biopsies, Susan J. Wilson and Stephen T. Holgate. Site-Directed Mutagenesis in Investigation of b-Adrenoreceptor Exosite, Stuart A. Green. Methods for Determining b2-Adrenoreceptor Genotype, Jane C. Dewar, Amanda P. Wheatley, and Ian P. Hall. Modulation of b-Adrenoceptor Expression, Judith C. W. Mak. Chemical Mutational Analysis of Glucocorticoid Receptor in Bronchial Asthma, Stephen J. Lane and Tak H. Lee. Histone Acetylation and Histone Deacetylation, Kazuhiro Ito, Peter J. Barnes, and Ian M. Adcock. Genome-Wide Search for Asthma Genes, Adel H. Mansur and John F. J. Morrison. Index.