Atomic Force Microscopy in Biomedical Research: Methods and Protocols by Pier Carlo BragaAtomic Force Microscopy in Biomedical Research: Methods and Protocols by Pier Carlo Braga

Atomic Force Microscopy in Biomedical Research: Methods and Protocols

EditorPier Carlo Braga, Davide Ricci

Hardcover | May 20, 2011

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With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life.  Atomic Force Microscopy in Biomedical Research: Methods and Protocols proves that this technology is no longer simply just another form of microscopy but has given rise to a completely new way of using microscopy that fulfils the dreams of all microscopists: being able to touch, move, and interact with the sample while it is being examined, thus making it possible to discover not only morphological but also chemical and physical structural information. Covering such topics as molecule imaging, nanoscale surface analysis and cellular imaging, force-spectroscopy, investigating drug action, and AFM as a nanotool, this volume features the most up-to-date techniques currently in use.  Written in the Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies.
Title:Atomic Force Microscopy in Biomedical Research: Methods and ProtocolsFormat:HardcoverDimensions:522 pagesPublished:May 20, 2011Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617791040

ISBN - 13:9781617791048

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Table of Contents

Part I: The Basics of Atomic Force Microscopy 1. How the Atomic Force Microscope Works            Bruno Torre, Davide Ricci, and Pier Carlo Braga 2. Measurement Methods in Atomic Force Microscopy            Bruno Torre, Claudio Canale, Davide Ricci, and Pier Carlo Braga 3. Recognizing and Avoiding Artifacts in Atomic Force Microscopy Imaging            Claudio Canale, Bruno Torre, Davide Ricci, and Pier Carlo Braga Part II: Molecule Imaging 4. Imaging the Spatial Orientation of Subunits within Membrane Receptors by Atomic Force Microscopy            Stewart M Carnally, J. Michael Edwardson, and Nelson P. Barrera 5. High Resolution Imaging of Immunoglobulin G (IgG) Antibodies and Other Biomolecules Using Amplitude Modulation Atomic Force Microscopy in Air            Sergio Santos and Neil H. Thomson 6. Atomic Force Microscopy of Ex vivo Amyloid Fibrils            Claudio Canale, Annalisa Relini, and Alessandra Gliozzi 7. Studying Collagen Self-Assembly by Time-Lapse High-Resolution Atomic Force Microscopy            Clemens M. Franz and Daniel J. Muller 8. Atomic Force Microscopy Imaging of Human Metaphase Chromosomes in Liquid            Osamu Hoshi and Tatsuo Ushiki 9. Atomic Force Microscopy of Proteasome Assemblies            Maria Gaczynska and Pawel A. Osmulski 10. Atomic Force Microscopy of Isolated Mitochondria            Bradley E. Layton and M. Brent Boyd 11. Imaging Native Membrane Proteins Using the Atomic Force Microscope            Andreas Engel Part III: Nanoscale Surface Analysis and Cell Imaging 12. Atomic Force Microscopy Investigation of Viruses            Alexander McPherson and Yurii G. Kuznetsov 13. Determination of the Kinetic On and -Off Rate of Single Virus-Cell Interactions            Christian Rankl, Linda Wildling, Isabel Neundlinger, Ferry Kienberger, Hermann Gruber, Dieter Blaas, and Peter Hinterdorfer 14. Atomic Force Microscopy as a Tool for the Study of the Ultrastructure of Trypanosomatid Parasites            Wanderley de Souza, Gustavo M. Rocha, Kildare Miranda, Paulo M. Bisch, and Gilberto Weissmuller 15. Normal and Pathological Erythrocytes Studied by Atomic Force Microscopy            Andreas Ebner, Hermann Schillers, and Peter Hinterdorfer 16. The Growth Cones of Living Neurons Probed by the Atomic Force Microscope            Davide Ricci, Massimo Grattarola, and Mariateresa Tedesco 17. Highlights on Ultrastructural Pathology of Human Sperm            Narahari V. Joshi, Ibis Cruz, and Jesus A. Osuna 18. High-speed Atomic Force Microscopy and Biomolecular Processes            Takayuki Uchihashi and Toshio Ando Part IV: Non-Topographical Applications (Force-Spectroscopy) 19. Atomic Force Microscopy in Mechanobiology: Measuring Micro-Elastic Heterogeneity of Living Cells            Evren U. Azeloglu and Kevin D. Costa 20. Force-Clamp Measurements of Receptor-Ligand Interactions            Félix Rico, Calvin Chu, and Vincent T. Moy 21. Measuring Cell Adhesion Forces: Theory and Principles            Martin Benoit and Christine Selhuber-Unkel 22. Nanoscale Investigation on E. coli Adhesion to Modified Silicone Surfaces            Ting Cao, Haiying Tang, Xuemei Liang, Anfeng Wang, Gregory W. Auner, Steven O. Salley, and K.Y. Simon Ng Part V: Investigating Drug Action 23. Imaging Bacterial Shape, Surface, and Appendages Before and After Treatment with Antibiotics            Pier Carlo Braga and Davide Ricci 24. Thymol-Induced Alterations in Candida albicans Imaged by Atomic Force Microscopy            Pier Carlo Braga and Davide Ricci 25. Atomic Force Microscope-Enabled Studies of Integrin-Extracellular Matrix Interactions in Vascular Smooth Muscle and Endothelial Cells            Zhe Sun and Gerald A. Meininger 26. Atomic Force Microscopy Studies on Circular DNA Structural Changes by Vincristine and Aspirin            Zhongdang Xiao, Lili Cao, Dan Zhu, and Zuhong Lu Part VI: Atomic Force Microscopy as a Nanotool 27. Combined Atomic Force Microscopy and Fluorescence Microscopy            Miklós S.Z. Kellermayer 28. Chemical Modifications of Atomic Force Microscopy Tips            Régis Barattin and Normand Voyer 29. Atomic Force Microscopy as Nanorobot            Ning Xi, Carmen Kar Man Fung, Ruiguo Yang, King Wai Chiu Lai, Donna H. Wang, Kristina Seiffert-Sinha, Animesh A. Sinha, Guangyong Li, and Lianqing Liu