Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Sequencing by Shaying ZhaoBacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Sequencing by Shaying Zhao

Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Sequencing

byShaying Zhao

Paperback | November 10, 2010

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Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.
Title:Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and SequencingFormat:PaperbackDimensions:360 pagesPublished:November 10, 2010Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372889

ISBN - 13:9781617372889

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Table of Contents

BAC Library Construction Kazutoyo Osoegawa and Pieter J. de Jong Construction of Small Genome BAC Library for Functional and Genomic Applications David Smajs, Steven J. Norris, and George M. Weinstock Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging Sangita Pal, Solida Mak, and George M. Weinstock Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions Natalay Kouprina, Vladimir N. Noskov, Maxim Koriabine, Sun-Hee Leem, and Vladimir Larionov Purification of BAC DNA Tim S. Poulsen Hybridization-Based Selection of BAC Clones Chang-Su Lim and Ung-Jin Kim Applications of Interspersed Repeat Sequence Polymerase Chain Reaction Heike Zimdahl, Claudia Gösele, Thomas Kreitler, and Margit Knoblauch BAC Mapping Using Fluorescence In Situ Hybridization Xiao-Ning Chen and Julie R. Korenberg High-Throughput BAC Fingerprinting Jacqueline Schein, Tamara Kucaba, Mandeep Sekhon, Duane Smailus, Robert Waterston, and Marco Marra BAC End Sequencing Tim S. Poulsen and Hans E. Johnsen Radiation Hybrid Mapping With BAC Ends Michael Olivier, Shannon Brady, and David R. Cox Shotgun Library Construction for DNA Sequencing Bruce A. Roe Rolling Circle Amplification for Sequencing Templates Paul F. Predki, Chris Elkin, Hitesh Kapur, Jamie Jett, Susan Lucas, Tijana Glavina, and Trevor Hawkins Transposon-Mediated Sequencing Rachel Reeg and Anup Madan Pyrosequencing: A Tool for DNA Sequencing Analysis Elahe Elahi and Mostafa Ronaghi Use of Fimers to Eliminate Polymerase Chain Reaction and Primer-Dimer Artifacts and to Increase Yield in BAC-Sequencing Reactions Andrei Malykh, Nikolai Polushin, Alexei Slesarev, and Sergei Kozyavkin Retrofitting BACs and PACs With LoxP Transposonsto Generate Nested Deletions Pradeep K. Chatterjee Preparing Nested-Deletion Template DNA for Field Inversion Gel Electrophoresis Analyses and Position-Specific End Sequencing With Transposon Primers Pradeep K. Chatterjee and Joseph C. Baker, Jr. BAC Finishing Strategies Christine Bird and Darren Grafham Using the TIGR Assembler in Shotgun Sequencing Projects Mihai Pop and Dan Kosack Finishing 'Working Draft' BAC Projects by Directed Sequencing With ThermoFidelase and Fimers Andrei Malykh, Olga Malykh, Nikolai Polushin, Sergei Kozyavkin, and Alexei Slesarev Optimized Multiplex Polymerase Chain Reaction: An Effective Method for Rapid Gap Closure Diana Radune and Hervé Tettelin Assembly of DNA Sequencing Data Jeremy Schmutz, Jane Grimwood, and Richard M. Myers Sequence Finishing Jeremy Schmutz, Jane Grimwood, and Richard M. Myers Quality Assessment of Finished BAC Sequences Jeremy Schmutz, Jane Grimwood, and Richard M. Myers Index