Bacterial Pathogenesis: Methods and Protocols by Frank DeLeoBacterial Pathogenesis: Methods and Protocols by Frank DeLeo

Bacterial Pathogenesis: Methods and Protocols

EditorFrank DeLeo

Paperback | December 9, 2010

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Bacterial infections affect world health today as a leading cause of morbidity and mortality. This book presents in-depth methods and state-of-the-art protocols for investigating specific mechanisms of pathogenesis for a wide range of bacteria. Written by experts in the field, this invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed to control infections.
Title:Bacterial Pathogenesis: Methods and ProtocolsFormat:PaperbackDimensions:336 pagesPublished:December 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617377511

ISBN - 13:9781617377518

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Table of Contents

Part I. Mechanisms of Bacterial Pathogenesis1 Isolation and Characterization of LipopolysaccharidesMichael A. Apicella2 Proteomic Analysis of Proteins Secreted by Streptococcus pyogenesMichelle A. Chaussee, Emily J. McDowell, and Michael S. Chaussee3 Proteomic Analysis to Investigate Regulatory Networks in Staphylococcus aureus Susanne Engelmann and Michael Hecker4 Microarray Comparative Genomic Hybridization for the Analysis of Bacterial Population Genetics and EvolutionCaitriona M. Guinane and J. Ross Fitzgerald5 Detection and Inhibition of Bacterial Cell-Cell CommunicationScott A. Rice, Diane McDougald, Michael Givskov, and Staffan Kjelleberg6 A System for Site-Specific Genetic Manipulation of the Relapsing Fever Spirochete Borrelia hermsiiJames M. Battisti, Sandra J. Raffel, and Tom G. Schwan7 Transposon Mutagenesis of the Lyme Disease Agent Borrelia burgdorferiPhilip E. Stewart and Patricia A. Rosa8 The Biofilm Exopolysaccharide Polysaccharide Intercellular Adhesin (PIA) - A Molecular and Biochemical Approach Cuong Vuong and Michael OttoPart II. Host-Pathogen Interaction9 Analysis of Staphylococcus aureus Gene Expression during PMN PhagocytosisJovanka M. Voyich, Dan E. Sturdevant, and Frank R. DeLeo10 Examining the Vector-Host-Pathogen Interface with Quantitative Molecular ToolsJason E. Comer, Ellen A. Lorange, and B. Joseph Hinnebusch11 Intracellular Localization of Brucella abortus and Francisella tularensis in Primary Murine MacrophagesJean Celli12 Rate and Extent of Helicobacter pylori PhagocytosisLee-Ann H. Allen13 Culture, Isolation and Labeling of Anaplasma Phagocytophilum for Subsequent Infection of Human Neutrophils Dori L. Borjesson14 Ultrastructural Analysis of Bacteria-Host Cell InteractionsDavid W. Dorward15 Infection of Human Monocyte-derived Macrophages with Coxiella burnetiiJeffrey G. Shannon and Robert A. Heinzen16 Infection of Epithelial Cells with Salmonella entericaOlivia Steele-Mortimer17 Determining the Cellular Targets of ROS in Borrelia burgdorferiJulie A. Boylan and Frank C. GherardiniPart III. Animal Models of Bacterial Infection18 Bioluminescent Imaging of Bacterial Biofilm Infections in vivoJagath L. Kadurugamuwa and Kevin P. Francis19 The Cotton Rat as a Model for Staphylococcus aureus Nasal Colonization in Humans (Cotton Rat S. aureus Nasal Colonization Model)John Kokai-Kun20 A Non-Human Primate Model of Acute Group A Streptococcus PharyngitisPaul Sumby, Anne H. Tart, and James M. MusserPart IV. Identification of Therapeutic Targets and Typing Methods21 Target-based Antimicrobial Drug DiscoveryLefa E. Alksne and Paul M. Dunman22 Sequence Analysis of the Variable Number Tandem Repeat in Staphylococcus aureus Protein A Gene: spa TypingBarun Mathema, Jose Mediavilla, and Barry N. Kreiswirth

Editorial Reviews

From the reviews:"An eclectic collection of papers ranges from those with limited general applications, to those with a much broader interest. . The additional notes in each paper are most useful, and may help those making use of these techniques avoid some of the frustrations associated with following methodologies in published journal papers. . a volume to dip into seeking inspiration for new techniques to apply to one's own favourite organism." (Jonathan Fletcher, Microbiology Today, July, 2008)"This book was written to bring scientists working in the field of bacterial pathogenesis up-to-date with both traditional tried-and-tested methods that have been in use for decades, and more recent technological advances and breakthroughs that have led to the development of new protocols. . On the whole this book is very informative and includes an extensive set of complex and detailed protocols. . I would recommend to anyone in the field or thinking of entering the field of bacterial pathogenesis." (Andrew Vaughan, Immunology News, August, 2008)"The 431st volume of Methods in Molecular Biology published by Humana Press is dedicated to the problems of bacterial pathogenesis. ... Each chapter is concluded by an exhaustive list of references. ... By its high level of interest and schematic approach the book is suitable not only for scientists dealing with bacterial pathogenesis ... but it can be recommended also for graduate students in microbiology who are about to start their scientific career." (K. Tyllovâ, Folia Microbiologica, Vol. 53, November, 2008)