Basic Laboratory Calculations For Biotechnology by Lisa A. Seidman

Basic Laboratory Calculations For Biotechnology

byLisa A. Seidman

Paperback | April 18, 2007

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To succeed in the lab, it is crucial to be comfortable with the math calculations that are part of everyday work. This accessible introduction to common laboratory techniques focuses on the basics, helping even readers with good math skills to practice the most frequently encountered types of problems. 


Discusses very common laboratory problems, all applied to real situations. Explores multiple strategies for solving problems for a better understanding of the underlying math. Includes hundreds of practice problems, all with solutions and many with boxed, complete explanations; plus hundreds of “story problems” relating to real situations in the lab.


 MARKET: A useful review for biotechnology laboratory professionals.

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Title:Basic Laboratory Calculations For BiotechnologyFormat:PaperbackDimensions:504 pages, 11 × 8.5 × 1.1 inPublished:April 18, 2007Publisher:Pearson EducationLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0132238101

ISBN - 13:9780132238106

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Writing a textbook in community psychology requires the resolution of two challenges. First, community psychology consists of a way of thinking more than a set of accumulated facts. Second, the field's values inform action as much as basic research and theory. Textbooks traditionally focus on received knowledge—the stable, time-tested, agreed-upon "facts" of a field. This cannot be done for community psychology, at least not in the same way. A hallmark of community psychology is the view of understanding as contextual. Knowledge develops within a sociocultural and historical setting, and science is not pure, objective, and value-free. Moreover, the goal of community psychology is not solely to increase understanding but to use our knowledge to create a more just and humane society. Community psychology's existence as a way of thinking and acting rather than a body of facts helps explain why there have been a dearth of textbooks, but it also makes the field immensely exciting. Guiding Principles and Orienting Concepts as the Organizational Schemes The organizing scheme of this textbook makes. clear community psychology's unique approach. The book is divided into two main sections: guiding principles, which anchor community psychologists to a coherent set of fundamental values, and orienting concepts, that allow us to remain open to paradigm-shaking perspectives. Throughout the years, community psychologists have delineated a set of principles that define our way of thinking. These principles differentiate community psychology from other areas of psychology. They give the field its singular character, instill a sense of identity and pride in its practitioners and permit the field to exist as a stable and circumscribed entity. Two introductory chapters ground the inquiry into community psychology. The first describes where community psychology came from and what it is. The second describes who community psychologists are and what we do. The next section consists of five chapters, each of which examines one of community psychology's guiding principle. The five principles are: A view of knowledge as fluid and value based; The belief that one cannot understand an individual without also understanding the many-leveled social contexts in which that individual lives; Attention to the voices of diverse (and often disenfranchised) groups; A commitment to improving the lives of individuals through intervention and social change; An emphasis on a strengths model rather than deficit model of mental health. While the guiding principles are the stable foundation on which the practice and research of community psychology build, orienting concepts allow the field to remain relevant in a changing world. Orienting concepts are frameworks that summarize past research and theory, give meaning to current knowledge, and guide future research and action. They put the guiding principles into action. Orienting concepts, unlike fundamental principles, are supposed to change. As our knowledge grows and Zeitgeists shift, so too do our orienting concepts. The field needs new orienting concepts to inspire new insights, bring new energy, and shake us out of our collective intellectual complacency Without new orienting concepts, community psychology's research and practice would become "one-sided" (Rappaport, 1981, p. Z1). The third section of this book describes the field's orienting concepts. Four chapters examine each orienting concept, thereby providing a review and application of the guiding principles, and a chronological overview of the development of the field. The orienting concepts are: Stress and coping Prevention Empowerment Resilience In keeping with community psychology's emphasis on the context of knowledge, each chapter begins with a description of the surrounding culture from which the chapter's ideas emerged and ends with a forward-looking section entitled The Promise of Community Psychology. In this section I examine an idea from the chapter that has captured my own imagination and may have implications for future community psychology. Thus, I have tried to attend to the past, present, and future of community psychology, balancing reviews of classic work, descriptions of the current state of the field, and emerging perspectives. Textbooks frequently present information as definitive, objective summaries of a field. My goal however, is not to line up silent and obedient facts that students can march-into examination booklets. I hope readers feel invited into a dialogue about community psychology that continues after the last word of this text is read. Toward this goal, I have provided numerous references as resources for future inquiries. Community psychology recognizes that researchers and practitioners are not disengaged observers of life, but active participants whose own beliefs and experiences influence their work. One goal of the field has been to resist the temptation to present scientific endeavors as value-free and to be candid instead about the viewpoints that inform our own understanding. In keeping with this perspective, I have adopted a personal approach to the material and relate how my experiences have influenced my thinking about community psychology. At the same time, I realize the limitations of one person's perspective (limitations that I expect will be even more apparent to readers), but I hope that my perspective provides a helpful window to the material and encourages readers to bring themselves and their varied experiences to the table as well. This book is not intended as a definitive attempt to cover the field, but as an invitation to students to discover the field and make it their own. Action Orientation Community psychology views knowledge as constructed and therefore focuses on ways of thinking rather than facts per se. Learning to think like a community psychologist is not, however, an end goal. Community psychology succeeds as a field only to the extent that its unique ways of thinking promote useful ways of acting in the world. The study of community psychology should result in a raised consciousness about the sorts of problems that require intervention and an increased awareness of how to intervene effectively. Community psychology's emphasis on the practical application of knowledge is at odds with the traditional view of academia. Webster's Dictionary offers the following among its definitions of academic: "theoretical without having an immediate or practical bearing... having no practical or useful significance." This view is not compatible with community psychology. Kurt Lewin, a forefather of the field, held that generating a knowledge base and effecting social change were two equally important and highly compatible goals of community research. Similarly, teaching in community psychology can and should be socially useful as well as theoretically meaningful. Thus, the textbook encourages informed student involvement in their communities. Students interested in community psychology have likely been attracted to the field because they have a good dose of inspiration to start with, believe that change is possible, and want to make a difference. This book succeeds if it fuels rather than stifles student creativity and activism. Photographs, cartoons, and other pedagogical devices are included to further engage the imagination. The dual interest in academics and action is also reinforced through the inclusion of Classic Research and Community Intervention boxes. The Classic Research boxes describe influential studies relevant to the chapter topic, sometimes in areas other than community psychology. The Community Intervention boxes show how the field's principles have been put into action by community psychologists and by others. (Admittedly, in a field that combines research and action, the distinction between research and intervention sometimes blurs.) Encouragement of student action permeates the text and becomes explicit at the end of each chapter in a section entitled Action Agenda. The activities in this section allow students to use information from the chapter as a basis for: (1) seeing themselves and their communities with new eyes; (2) generating ideas for needed changes; and (3) taking initial steps toward change. Certainly not all of these activities can be completed in the span of a semester, but hopefully they present some provocative ideas for living out community psychology in the process of studying it. Attention in this section to the dynamics of the classroom and the use of cooperative learning also allows students to explore ways of creating a community in the classroom as a microcosm of the larger society. In summary, this .textbook explores the past, present, and future of community psychology. The guiding principles and orienting concepts of the field constitute the main organizing structure. Readers are encouraged to think about the field, learn about themselves, and apply their knowledge to the world around them.

Table of Contents

Unit I: Brief Review of Some Basic Math Techniques

1. Exponents and Scientific Notation

2. Logarithms

3. Units of Measurement

4. Measurements and Significant Figures

5. Using Equations to Describe a Relationship


Unit II: Applications of Proportional Relationships in the Biology Laboratory

6. Ratios and Proportions

7. Unit Conversions

8. Density

9. Dosages

10. Percents

11. Introduction to Concentration Problems

12. Preparing Laboratory Solutions That Contain One Solute

13. Dilutions

14. Preparing Laboratory Solutions That Contain More Than One Solute


Unit III: Describing Relationships with Equations and Graphs

15. Graphing Linear Equations

16. Spectrophotometry

17. Graphing Exponential Equations


Unit IV: Introduction to Descriptive Statistics

18. Measures of Central Tendency

19. Measures of Dispersion

20. Graphical Methods of Describing Data


Unit V. Biotechnology Applications

21. Calculations Relating to Animal Cell Culture

22. Amount and Concentration of Nucleic Acids

23. Calculations Relating to Common Molecular Biology Techniques

24. The Polymerase Chain Reaction

25. Calculations Relating to Protein Purification and Analysis

Editorial Reviews

"...Rudkin has produced a book that takes seriously the idea of answering the questions of what is community psychology, who are the community psychologists, and what values underlie their work. Her division of the ,field into five guiding principles and four orienting concepts that summarize critical areas of research and theory is masterful. She has managed to incorporate these principles and concepts in an integrated, insightful, and creative manner to provide a broad overview of the field as it exists today... The book is a remarkable achievement. I fully expect it to play a major role in attracting the next generation of students to become community psychologists. Read it and enjoy it!" — N. Dickon Reppucci — Director of the Community Psychology Program, University of Virginia (excerpted from the Foreword to this text)"Overall, this is an excellent book. It was wonderful to see the integration of classic and new theory, methods and content in one text." — Jennifer Woolard, Georgetown University"The competitive advantage of this book is that its content and flavor are well-grounded in the community psychology ethos. It also does a better job at including issues of diversity." — Kelly L. Hazel, Community Psychology Graduate Program Coordinator, Metropolitan State University"I am most pleased to endorse the textbook, Community Psychology: Guiding Principles and Orienting Concepts, by Jennifer Kofkin Rudkin.This text is one that we have decided to adopt for our undergraduate Community Psychology lecture classes here at Illinois, and I have personally been assigning the text to first year Clinical/Community graduate students as a supplement to their reading of articles and chapters. I have found this text to provide a thoughtful introduction to students at any level who need to quickly grasp the broad scope of community psychology, its values, range of research and action. The book is well written, explains complicated concepts and is a genuine scholarly contribution to the literature. It does more than report the work of others; it asserts a point of view and a conceptual framework while providing many concrete illustrations of community research and action.Please feel free to quote any or all of this endorsement in advertising. I would be glad to add additional thoughts if that is helpful to you. This is a book that I hope will become widely read." — Julian Rappaport, Professor of Psychology University of Illinois at Urbana Champaign