Basic Science Methods For Clinical Researchers by Morteza Jalali

Basic Science Methods For Clinical Researchers

byMorteza JalaliEditorFrancesca Yvonne Lou Saldanha, Mehdi Jalali

Paperback | April 4, 2017

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Basic Science Methods for Clinical Researchersaddresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data.

This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science.



  • Serves as a helpful guide for clinical researchers who lack a conventional science background
  • Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms
  • Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data
  • Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

About The Author

Morteza Jalali is a British doctor-preneur who is fascinated by transformational healthcare innovation through synergies between clinicians, patients, industry and scientists. Born in Manchester, United Kingdom, at the age of 18 he commenced a medical degree at Manchester Medical School and undertook dedicated research training at The ...

Details & Specs

Title:Basic Science Methods For Clinical ResearchersFormat:PaperbackDimensions:382 pages, 8.75 × 6.35 × 0.68 inPublished:April 4, 2017Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0128030771

ISBN - 13:9780128030776

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Table of Contents

1. The Polymerase Chain Reaction: PCR, qPCR, and RT-PCR 2. Methods of Cloning 3. Whole-Mount In Situ Hybridization and a Genotyping Method on SingleXenopusEmbryos 4. Microarrays: An Introduction and Guide to Their Use 5. Analysis of Human Genetic Variations Using DNA Sequencing 6. Western Blot 7. The Enzyme-Linked Immunosorbent Assay: The Application of ELISA in Clinical Research 8. Immunofluorescence 9. Cell Culture: Growing Cells as Model Systems In Vitro 10. Flow Cytometry 11. Transfection 12. In Vivo Animal Modeling:Drosophila13. Zebrafish as a Research Organism:Danio rerioin Biomedical Research 14. Xenopus as a Model Organism for Biomedical Research 15. Basic Mouse Methods for Clinician Researchers: Harnessing the Mouse for Biomedical Research