Basic Science Methods For Clinical Researchers

Paperback | April 15, 2017

byMorteza JalaliEditorFrancesca Yvonne Lou Saldanha, Mehdi Jalali

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Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice GLP

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Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations i...

Morteza Jalali is a British doctor-preneur who is fascinated by transformational healthcare innovation through synergies between clinicians, patients, industry and scientists. Born in Manchester, United Kingdom, at the age of 18 he commenced a medical degree at Manchester Medical School and undertook dedicated research training at The ...
Format:PaperbackDimensions:352 pages, 8.75 × 6.35 × 0.68 inPublished:April 15, 2017Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0128030771

ISBN - 13:9780128030776

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Table of Contents

Section I: Gene 1. PCR, qPCR, and RT-PCR 2. Methods of Cloning 3. In situ Hybridisation 4. Microarray

Section II: Protein 5. Western Blot 6. Enzyme-Linked Immunosorbent Assay 7. Immunofluorescence

Section III: Cell 8. Cell Culture 9. Flow Cytometry 10. Transfection 11. Functional Assays

Section IV: System 12. In vivo Animal Modelling: Drosophila 13. In vivo Animal Modelling: Danio 14. In vivo Animal Modelling: Xenopus 15. In vivo Animal Modelling: Mouse

Appendices Appendix A: Legal framework on the scientific use of animals in research Appendix B: Regulatory framework of stem cell research