Bioconjugation: Methods And Protocols by Sam MassaBioconjugation: Methods And Protocols by Sam Massa

Bioconjugation: Methods And Protocols

bySam MassaEditorNick Devoogdt

Hardcover | September 16, 2019

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This book explores well-established and emerging conjugation strategies that are relevant for proteins used in the field of precision medicine, focusing on techniques that are suitable for antibodies, antibody-fragments such as Fabs, scFvs, or nanobodies, scaffold proteins such as FN3 or DARPin, peptides, or model proteins. Although centered on the development of bioconjugates rather than their application, most protocols also show the conjugation of the targeting vehicle to a diagnostic or therapeutic entity, with the end-product most often being an antibody-drug conjugate, an optical probe, a nanomedicine, or a radiopharmaceutical. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical,Bioconjugation: Methods and Protocolsis an ideal guide for researchers looking toward precision medicine in order to expand the vital field of drug discovery.
Title:Bioconjugation: Methods And ProtocolsFormat:HardcoverDimensions:317 pagesPublished:September 16, 2019Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1493996533

ISBN - 13:9781493996537


Table of Contents

1. Site-Specific Conjugation of Thiol-Reactive Cytotoxic Agents to Non-Native Cysteines of Engineered Monoclonal Antibodies

            Stephan Dickgiesser, Roland Kellner, Harald Kolmar, and Nicolas Rasche


2. Application of Next Generation Maleimides (NGMs) to Site-Selective Antibody Conjugation

            Maurício Morais, Nafsika Forte, Vijay Chudasama, and James R. Baker


3. Lysine Bioconjugation on Native Albumin with a Sulfonyl Acrylate Reagent

            Maria J. Matos, Gonzalo Jiménez-Osés, and Gonçalo J.L. Bernardes


4. Engineering Dual-Variable-Domains for the Generation of Site-Specific Antibody-Drug Conjugates

            Alex R. Nanna and Christoph Rader


5. Efficient Production of Homogeneous Lysine-Based Antibody Conjugates Using Microbial Transglutaminase

            Jared L. Spidel and Earl F. Albone


6. Engineering Antibodies with C-Terminal Sortase-Mediated Modification for Targeted Nanomedicine

            Rania A. Hashad, Jaclyn L. Lange, Natasha C.W. Tan, Karen Alt, and Christoph E. Hagemeyer


7. CRISPR/Cas9-Mediated Genetic Engineering of Hybridomas for Creation of Antibodies that Allow Site-Specific Conjugation

            Makan Khoshnejad, Jacob S. Brenner, Hamideh Parhiz, and Vladimir R. Muzykantov


8. Site-Specific Modification of Proteins via Trypsiligase

            Sandra Liebscher and Frank Bordusa


9. Site-Selective Functionalization of Nanobodies Using Intein-Mediated Protein Ligation for Innovative Bioconjugation

            Geert-Jan Graulus, Duy Tien Ta, Huong Tran, Rebekka Hansen, Brecht Billen, Erik Royackers, Jean-Paul Noben, Nick Devoogdt, Serge Muyldermans, Wanda Guedens, and Peter Adriaensens


10. Site-Specific Bioconjugation Using SMARTag 'Technology: A Practical and Effective Chemoenzymatic Approach to Generate Antibody-Drug Conjugates

            Junjie Liu, Robyn M. Barfield, and David Rabuka


11. Protein Labeling and Bioconjugation Using N-Myristoyltransferase

            Karin F.K. Ejendal, Julia G. Fraseur, and Tamara L. Kinzer-Ursem


12. One-Step Fluorescent Protein Labeling by Tubulin Tyrosine Ligase

            Dominik Schumacher, Heinrich Leonhardt, Christian P.R. Hackenberger, and Jonas Helma


13. Enzymatically Catalyzed Radiofluorination of Biomolecules

            Christopher R. Drake, Michael J. Evans, and Henry F. VanBrocklin


14. Site-Selective Enzymatic Labeling of Designed Ankyrin Repeat Proteins Using Protein Farnesyltransferase

            Yi Zhang, Shelby Auger, Jonas V. Schaefer, Andreas Plückthun, and Mark D. Distefano


15. Homogeneous Antibody-Drug Conjugates via Glyco-Engineering

            Feng Tang, Wei Shi, and Wei Huang


16. Labeling Proteins at Site-Specifically Incorporated 5-Hydroxytryptophan Residues Using a Chemoselective Rapid Azo-Coupling Reaction

            Partha Sarathi Addy, Sarah B. Erickson, James S. Italia, and Abhishek Chatterjee


17. Facile Site-Specific Multi-Conjugation Strategies in Recombinant Proteins Produced in Bacteria

            Hannes Merten, Jonas V. Schaefer, Fabian Brandl, Uwe Zangemeister-Wittke, and Andreas Plückthun


18. Site-Specific Photocrosslinking to Immunoglobulin G Using Photoreactive Antibody-Binding Domains

            Fabiana Zappala and Andrew Tsourkas


19. SpyTag-SpyCatcher Chemistry for Protein Bioconjugation In Vitro and Protein Topology Engineering In Vivo

            Xiao-Wei Wang and Wen-Bin Zhang


20. FN3 Protein Conjugates for Cancer Diagnosis and Imaging Studies

            Arutselvan Natarajan and Lotfi Abou-Elkacem