Biolistic DNA Delivery: Methods and Protocols by Stephan SudoweBiolistic DNA Delivery: Methods and Protocols by Stephan Sudowe

Biolistic DNA Delivery: Methods and Protocols

EditorStephan Sudowe, Angelika B. Reske-Kunz

Hardcover | October 29, 2012

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Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun.  Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. All-inclusive and cutting-edge,  Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field.
Title:Biolistic DNA Delivery: Methods and ProtocolsFormat:HardcoverDimensions:429 pages, 10 × 7.01 × 0.01 inPublished:October 29, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:162703109X

ISBN - 13:9781627031097

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Table of Contents

Part I: Biolistic DNA Delivery in Plant Science and Agricultural Biotechnology 1. Comparison Between Agrobacterium-Mediated and Direct Gene Transfer Using the Gene Gun            Caixia Gao and Klaus K. Nielsen 2. Transient Gene Expression in Epidermal Cells of Plant Leaves by Biolistic DNA Delivery            Shoko Ueki, Shimpei Magori, Benoît Lacroix, and Vitaly Citovsky 3. Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues            Mulpuri Sujatha and K.B.R.S. Visarada 4. Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-Vacuum Gene Gun (HandyGun)            Anssi L. Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, and Jari P.T. Valkonen 5. HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies            Victor Gaba, Moshe Lapidot, and Amit Gal-On 6. Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun            Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, and Kathy E. Schwinn Part II: Biolistic DNA Delivery in Nematodes 7. Biolistic Transformation of Caenorhabditis elegans            Meltem Isik and Eugene Berezikov 8. Improved Vectors for Selection of Transgenic Caenorhabditis elegans            Annabel A. Ferguson, Liquan Cai, Luv Kashyap, and Alfred L. Fisher 9. Biolistic Transformation of Brugia malayi            Tarig B. Higazi and Thomas R. Unnasch Part III: Biolistic DNA Delivery in Mammalian Cells In Vitro 10. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells            Xiongwei Li, Masaki Uchida, H. Oya Alpar, and Peter Mertens 11. Biolistic Transfection of Tumor Tissue Samples            Kandan Aravindaram, Shu-Yi Yin, and Ning-Sun Yang 12. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes            David F. Steele, Ying Dou, and David Fedida 13. Biolistic Transfection of Neurons in Organotypic Brain Slices            John A. O'Brien and Sarah C.R. Lummis Part IV: Biolistic DNA Delivery in Mammalian Cells In Vivo 14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun            Meng-Chi Yen and Ming-Derg Lai 15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery            Ahmad Jalili 16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs            Steve Pascolo 17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter            Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz 18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice            Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus Part V: Biolistic DNA Vaccination in Animal Disease Models 19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets            Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller 20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models            Gustaf Ahlén, Matti Sällberg, and Lars Frelin 21. Gene Gun Immunization to Combat Malaria            Elke S. Bergmann-Leitner and Wolfgang W. Leitner 22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination            Toshi Nagata and Yukio Koide 23. Biolistic DNA Vaccination Against Trypanosoma Infection            Marianne Bryan, Siobhan Guyach, and Karen A. Norris 24. Biolistic DNA Vaccination Against Melanoma            Julia Steitz and Thomas Tüting 25. Biolistic DNA Vaccination Against Cervical Cancer            Michal Smahel 26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy            Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe 27. Safety Assessment of Biolistic DNA Vaccination            Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek Part VI: Related Applications for Biolistic Delivery of Molecules 28. DiOlistics: Delivery of Fluorescent Dyes into Cells            Nyssa Sherazee and Veronica A. Alvarez 29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI)            Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss 14. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun            Meng-Chi Yen and Ming-Derg Lai 15. Chemokine Overexpression in the Skin by Biolistic DNA Delivery            Ahmad Jalili 16. Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs            Steve Pascolo 17. Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter            Yvonne Höhn, Stephan Sudowe, and Angelika B. Reske-Kunz 18. Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice            Dirk Bauer, Susanne Wasmuth, Mengji Lu, and Arnd Heiligenhaus Part V: Biolistic DNA Vaccination in Animal Disease Models 19. Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets            Eric J. Yager, Cristy Stagnar, Ragisha Gopalakrishnan, James T. Fuller, and Deborah H. Fuller 20. Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models            Gustaf Ahlén, Matti Sällberg, and Lars Frelin 21. Gene Gun Immunization to Combat Malaria            Elke S. Bergmann-Leitner and Wolfgang W. Leitner 22. Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination            Toshi Nagata and Yukio Koide 23. Biolistic DNA Vaccination Against Trypanosoma Infection            Marianne Bryan, Siobhan Guyach, and Karen A. Norris 24. Biolistic DNA Vaccination Against Melanoma            Julia Steitz and Thomas Tüting 25. Biolistic DNA Vaccination Against Cervical Cancer            Michal Smahel 26. Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy            Verena Raker, Joachim Maxeiner, Angelika B. Reske-Kunz, and Stephan Sudowe 27. Safety Assessment of Biolistic DNA Vaccination            Barbara Langer, Matthias Renner, Jürgen Scherer, Silke Schüle, and Klaus Cichutek Part VI: Related Applications for Biolistic Delivery of Molecules 28. DiOlistics: Delivery of Fluorescent Dyes into Cells            Nyssa Sherazee and Veronica A. Alvarez 29. Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI)            Sandra Scheiblhofer, Uwe Ritter, Josef Thalhamer, and Richard Weiss            Mulpuri Sujatha and K.B.R.S. Visarada