Biophotonics, Part a

Other | February 1, 2003

byMarriott, Gerard, Gerard Marriott

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today truly an essential publication for researchers in all fields of life sciences.

* Discusses optical instrumentation for imaging, screening and diagnosis in molecules, tissues, and cells
* Covers the development and application of optical probes and techniques for imaging and drug screening
* Investigates the structure and dynamics of biomolecular systems, screening and drug discovery, and the diagnosis and treatment of disease

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From the Publisher

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them ...

Format:OtherDimensions:714 pages, 1 × 1 × 1 inPublished:February 1, 2003Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0080522580

ISBN - 13:9780080522586

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Table of Contents

Fluorescence: Basic Concepts, Practical Aspects, and Some Anecdotes.
Fluorescence Sensing Methods.
Bioluminescence and Chemiluminescence.
Fluorescent Amino Acid Analogs.
Fluorescent Nucleotides: Synthesis and Characterization.
Photophysics of Green and Red Fluorescent Proteins: Implications for Quantitative Microscopy.
Development of Genetically Encoded Fluorescent Indicators for Calcium.
Development and Application of Caged Calcium.
Application of Fluorescent Probes to Study Mechanics and Dynamics of Ca2+-Triggered Synaptotagmin C2 Domain-Membrane Interactions.
Caging Proteins through Unnatural Amino Acid Mutagenesis.
Preparation and Light-Directed Activation of Caged Proteins.
Bioluminescence Resonance Energy Transfer: Monitoring Protein-Protein Interactions in Living Cells.
Structure-Function Relationships in Metalloproteins.
Spectroscopy and Microscopy of Cells and Cell Membrane Systems.
Photonics for Biologists.
Imaging at Low Light Levels with Cooled and Intensified Charge-Coupled Device Cameras.
Filters and Mirrors for Applications in Fluorescence Microscopy.
Resolution in Optical Microscopy.
Video-Rate Confocal Microscopy.
Giant Vesicles, Laurdan, and Two-Photon Fluorescence Microscopy: Evidence of Lipid Lateral Separation in Bilayers.
Biological Near-Field Microscopy.
Fluorescence Lifetime-Resolved Imaging: Measuring Lifetimes in an Image.
Fluorescence Resonance Energy Transfer Imaging Microscopy.
FRET Imaging Microscopy.
Fluorescence Resonance Energy Transfer Imaging Microscopy and Fluorescence Polarization Imaging Microscopy.
Homo-FRET versus Hetero-FRET to Probe Homodimers in Living Cells.
Spinning Disk Confocal Microscope System for Rapid High Resolution, Multimode, Fluorescence Speckle Microscopy and Green Fluorescent Protein Imaging in Living Cells.
Single-Particle Tracking Image Microscopy.
Diffusion in Cells Measured by Fluorescence Recovery after Photobleaching, etc.