Blood'Brain Barrier: Biology and Research Protocols by Sukriti NagBlood'Brain Barrier: Biology and Research Protocols by Sukriti Nag

Blood'Brain Barrier: Biology and Research Protocols

EditorSukriti Nag

Paperback | November 9, 2010

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A first-time collection of cutting-edge morphologic, permeability, transport, in vitro, and molecular techniques to study the workings of the blood-brain barrier in health and disease. Described in step-by-step detail, each readily reproducible method includes an explanation of the theoretical basis of the method, a discussion of its advantages and limitations, notes on avoiding pitfalls, and examples of results obtained using the technqiue. These powerful tools can be used to isolate undiscovered molecules, deepen our understanding of their role in health and disease, and identify targets for future gene therapy and therapeutic intervention.

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Title:Blood'Brain Barrier: Biology and Research ProtocolsFormat:PaperbackDimensions:564 pages, 10 × 7.01 × 0 inPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617373397

ISBN - 13:9781617373398

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Table of Contents

Part I. Tissue TechniquesMorphology and Molecular Properties of Cellular Components of Normal Cerebral VesselsSukriti NagStudies of Cerebral Vessels by Transmission Electron Microscopy and MorphometrySukriti NagFreeze-Fracture Studies of Cerebral Endothelial Tight JunctionsHartwig Wolburg, Stefan Liebner, and Andrea LippoldtStudies of Cerebral Endothelium by Scanning and High-Voltage Electron MicroscopyAlbert S. Lossinsky and Richard R. ShiversDetection of Endothelial/Lymphocyte Interaction in Spinal Cord Microvasculature by Intravital VideomicroscopyBritta Engelhardt, Peter Vajkoczy, and Melanie LaschingerPart II. Blood-Brain Barrier Permeability TechniquesPathophysiology of Blood-Brain Barrier BreakdownSukriti NagPial Vessel Permeability to Tracers Using Cranial WindowsWilliam G. MayhanBlood-Brain Barrier Permeability Using Tracers and ImmunohistochemistrySukriti NagUltracytochemical Studies of the Compromised Blood-Brain BarrierSukriti NagImmunogold Detection of Microvascular Proteins in the Compromised Blood-Brain BarrierEain M. Cornford, Shigeyo Hyman, and Marcia E. CornfordMeasurement of Blood-Brain and Blood-Tumor Barrier Permeabilities with [14C]-Labeled TracersKamlesh Asotra, Nagendra Ningaraj, and Keith L. BlackPart III. Blood-Brain Barrier Transport TechniquesA Review of Blood-Brain Barrier Transport TechniquesQuentin R. SmithIn Situ Brain Perfusion TechniqueQuentin R. Smith and David D. AllenBrain Efflux Index Method: Characterization of Efflux Transport Across the Blood-Brain BarrierHiroyuki Kusuhara, Tetsuya Terasaki, and Yuichi SugiyamaTransport Studies Using Membrane VesiclesDarryl R. Peterson and Richard A. HawkinsDrug Transport Studies Using Quantitative MicrodialysisHaiqing Dai and William F. ElmquistSingle-Pass Dual-Label Indicator Method: Blood-to-Brain Transport of Glucose and Short-Chain Monocarboxylic AcidsMichelle A. Puchowicz, Kui Xu, and Joseph C. LaMannaProtein Transport in Cerebral Endothelium: In Vitro Transcytosis of TransferrinLaurence Fenart and Roméo CecchelliTransport Across the Choroid Plexuses In Vivo and In VitroNathalie Strazielle and Jane Elizabeth PrestonPart IV. In Vitro TechniquesAn Overview of In Vitro Techniques for Blood-Brain Barrier StudiesAndreas Reichel, David J. Begley, and N. Joan AbbottIsolation and Characterization of Human Brain Endothelial CellsKaterina Dorovini-Zis, Rukmini Premeya, and Hanh HuynhHuman Brain Microvessel Endothelial Cell and Leukocyte InteractionsJacqueline Shukaliak-Quandt, Donald Wong, and Katerina Dorovini-ZisDevelopment and Characterization of Immortalized Cerebral Endothelial Cell LinesPierre-Olivier Couraud, John Greenwood, Françoise Roux, and Pete AdamsonIsolation and Characterization of Retinal Endothelial CellsDavid A. Antonetti and Ellen B. WolpertIsolation and Characterization of Cerebral Microvascular PericytesPaula Dore-DuffyPart V. Molecular TechniquesMolecular Biology of the Blood-Brain BarrierWilliam M. PardridgeBlood-Brain Barrier GenomicsRuben J. BoadoDynamic In Vitro Model of the Blood-Brain Barrier: Gene Profiling Using cDNA Microarray AnalysisMatteo Marroni, Kelly M. Kight, Mohammed Hossain, Luca Cucullo, Shailesh Y. Desai, and Damir JanigroPhage Display Technology for Identifying Specific Antigens on Brain Endothelial CellsJamshid Tanha, Arumugam Muruganandam, and Danica StanimirovicmRNA Detection in Cerebral Vessels by Nonradioactive In Situ HybridizationDavid Zagzag and Wai ChanProteomics of Brain Endothelium: Separation of Proteins by Two-Dimensional Gel Electrophoresis and Identification by Mass SpectrometryReiner F. Haseloff, Eberhard Krause, and Ingolf E. BlasigImmunoblot Detection of Brain Vascular ProteinsChristian T. Matson and Lester R. DrewesImmunohistochemical Detection of Endothelial ProteinsSukriti NagPart VI. Genetically Altered MiceCerebral Vascular Function in Genetically Altered MiceFrank M. FaraciGeneration of Transgenic Mice by Pronuclear InjectionAnnette Damert and Heike Kusserow. Mouse Genome Modification, Richard RozmahelIndex

Editorial Reviews

"An outstanding addition to the field! The book is unique in its organization and ease of reading and use as research resource. In my view, this book will emerge as one of the more cited and critical sources of information for all researchers exploring the nature of the blood-brain barrier."-Doody's Health Science Book Review Journal