Bone Research Protocols by Miep H. HelfrichBone Research Protocols by Miep H. Helfrich

Bone Research Protocols

EditorMiep H. Helfrich, Stuart H. Ralston

Paperback | November 9, 2010

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A collection of the latest laboratory techniques for the study of bone and bone tissue. Described in step-by-step detail, these readily reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological and ultrastructural analysis, methods for the measurement of bone strength and for mechanical studies, and how to use digital imaging techniques in the analysis of bone.
Title:Bone Research ProtocolsFormat:PaperbackDimensions:462 pagesPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617373214

ISBN - 13:9781617373213

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Table of Contents

Part I. Culture of Osteoblasts and OsteocytesHuman Osteoblast CultureJames A. GallagherOsteoblast Isolation from Murine Calvariae and Long BonesAstrid Bakker and Jenneke Klein-NulendMineralizing Fibroblast-Colony-Forming AssaysAndrew Scutt, Lynsey Reading, Nanette Scutt, and Karen StillOsteocyte Isolation and CulturePeter J. Nijweide, Arie van der Plas, Marcel J. Alblas, and Jenneke Klein-NulendPart II. Culture of Osteoclasts. Isolated Osteoclast CulturesAstrid Hoebertz and Timothy R. ArnettPrimary Isolation and Culture of Chicken OsteoclastsPatricia Collin-Osdoby, Fred Anderson, and Philip OsdobyIsolation and Purification of Rabbit OsteoclastsFraser P. Coxon, Julie C. Frith, Helena L. Benford, and Michael J. RogersGenerating Human Osteoclasts from Peripheral BloodAfsie Sabokbar and Nicholas A. AthanasouGenerating Human Osteoclasts from Bone Marrow and Peripheral BloodAdrienne M. Flanagan and Helen M. MasseyGenerating Murine Osteoclasts from Bone MarrowNaoyuki Takahashi, Nobuyuki Udagawa, Sakae Tanaka, and Tatsuo SudaOsteoclast Formation in the Mouse Coculture AssayRobert J. van 't HofRANKL-Mediated Osteoclast Formation from Murine RAW 264.7 CellsPatricia Collin-Osdoby, Xuefeng Yu, Hong Zheng, and Philip OsdobyPart III. Bone Organ CultureAnalysis of Osteoclast Function in Mouse Calvarial CulturesMichael J. Marshall and Marit N. RowlandsAssessing Bone Formation Using Mouse Calvarial Organ CultureI. Ross GarrettPart IV. Histological and Ultrastructural TechniquesIn Situ Hybridization and In Situ Reverse Transcription Polymerase Chain Reaction in Human Bone SectionsAndrew P. Mee and Judith A. HoylandTechniques for the Study of Apoptosis in BoneBrendon S. Noble and Hazel Y. StevensProtein Localization in Wax-Embedded and Frozen Sections of Bone Using ImmunohistochemistrySharyn BordDetection of Noncollagenous Bone Proteins in Methylmethacrylate-Embedded Human Bone SectionsJohannes P. T. M. van Leeuwen and Pieter DerkxFluorescence Imaging of Bone-Resorbing Osteoclasts by Confocal MicroscopyStephen A. Nesbitt and Michael A. HortonBone HistomorphometryShobna Vedi and Juliet CompstonTransmission Electron Microscopy of BoneVincent Everts, Anneke Niehof, and Wouter BeertsenScanning Electron Microscopy of BoneDeborah Marshall, Miep H. Helfrich, and Richard M. AspdenPart V. Imaging TechniquesBone Measurement by Peripheral Quantitative Computed Tomography in RodentsJürg A. GasserPart VI. In Vivo Techniques. Studies of Local Bone Remodeling: The Calvarial Injection AssayRobert J. van 't Hof, Claire E. Clarkin, and Kenneth J. ArmourInflammation-Induced Osteoporosis: The IMO ModelKenneth J. Armour and Katharine E. ArmourOvariectomy and Estrogen Replacement in RodentsJade W. M. ChowPart VII. Mechanical Loading TechniquesMechanical Testing of Bone Ex VivoRichard M. AspdenBone Cell Responses to Fluid FlowHazel Y. Stevens and John A. FrangosMethods for Analyzing Bone Cell Responses to Mechanical Loading Using In Vitro Monolayer and Organ Culture ModelsAndrew A. Pitsillides, Victoria Das-Gupta, Dominic Simon, and Simon C. F. RawlinsonPart VIII. Molecular AnalysisExtraction of Nucleic Acids from BoneTracy L. Stewart and Val MannAnalysis of Gene Expression in Bone by Quantitative RT-PCRDeborah IrelandIndex

Editorial Reviews

"Bone Research Protocols is a most welcome compilation of methods. In it, many of the most productive laboratories in some of the most challenging areas of cell biology reveal detailed protocols that have been used to solve key problems. Short of spending a sabbatical in one of the eminent laboratories that contributed, this volume provides the best possible introduction to these methods." - TRENDS in Endocrinology and Metabolism