Brain Development: Methods and Protocols by Simon G. SprecherBrain Development: Methods and Protocols by Simon G. Sprecher

Brain Development: Methods and Protocols

EditorSimon G. Sprecher

Hardcover | September 19, 2013

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Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes.  Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models.  Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.
Title:Brain Development: Methods and ProtocolsFormat:HardcoverDimensions:305 pagesPublished:September 19, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627036547

ISBN - 13:9781627036542

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Table of Contents

Part I: Drosophila Protocols 1. Immunostaining of the Developing Embryonic and Larval Drosophila Brain            Danielle C. Diaper and Frank Hirth 2. Non-Fluorescent RNA In Situ-Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila            David Jussen and Rolf Urbach 3. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI-Labeling            Karoline F. Kraft and Rolf Urbach 4. Flybow to Dissect Circuit Assembly in the Drosophila Brain            Nana Shimosako, Dafni Hadjieconomou, and Iris Salecker 5. Immunofluorescent Labeling of Neural Stem Cells in the Drosophila Optic Lobe            Benjamin Perruchoud and Boris Egger 6. Using MARCM to Study Drosophila Brain Development            Gudrun Viktorin Part II: Other Arthropods 7. Dye Coupling and Immunostaining of Astrocyte-Like Glia Following Intracellular Injection of Fluorochromes in Brain Slices of the Grasshopper, Schistocerca gregaria            George Boyan and Yu Liu Part III: Molluscs 8. Methods in Brain Development of Molluscs            Andreas Wanninger and Tim Wollesen Part IV: Xenopus Protocols 9. In Situ Hybridization and Immunostaining of Xenopus Brain            Kai-li Liu, Xiu-mei Wang, Zi-long Li, and Ying Liu 10. Microinjection Manipulations in the Elucidation of Xenopus Brain Development            Cristine Smoczer, Lara Hooker, Saqib S. Sachani, and Michael J. Crawford 11. Morpholino Studies in Xenopus Brain Development            Jennifer E. Bestman and Hollis T. Cline Part V: Zebrafish Protocols 12. Sensitive Whole-Mount Fluorescent In Situ Hybridization in Zebrafish Using Enhanced Tyramide Signal Amplification            Gilbert Lauter, Iris Söll, and Giselbert Hauptmann 13. Dynamic Neuroanatomy at Subcellular Resolution in the Zebrafish            Adèle Faucherre and Hernán López-Schier 14. Anatomical Dissection of Zebrafish Brain Development            Katherine J. Turner, Thomas G. Bracewell, and Thomas A. Hawkins Part VI: Chicken Protocols 15. Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain            Richard P. Tucker and Qizhi Gong 16. Transplantation of Neural Tissue: Quail-Chick Chimeras            Andrea Streit and Claudio D. Stern 17. RNAi-Based Gene Silencing in Chicken Brain Development            Irwin Andermatt and Esther T. Stoeckli Part VII: Mouse Protocols 18. Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development            Jinling Liu and Aimin Liu 19. In Utero Electroporation to Study Mouse Brain Development            Emilie Pacary and François Guillemot 20. The Cre/Lox System to Assess the Development of the Mouse Brain            Claudius F. Kratochwil and Filippo M. Rijli