Cajal and de Castros Neurohistological Methods by Miguel A. Merchan

Cajal and de Castros Neurohistological Methods

byMiguel A. Merchan, Javier De Felipe, Fernando De Castro

Hardcover | March 16, 2016

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Cajal and De Castro's Neurohistological Methods provides the first English translation of Fernando de Castro's 1933 publication "Elementos de Tecnica Micrografica del Sistema Nervioso". A student of the famed founder of modern neuroscience, with Santiago Ramon y Cajal also serving as theEditor of the original text, Fernando de Castro recorded all the various protocols that had been used in his laboratory by his students in order to provide a manual of histological procedures specifically designed for the fine structure of the nervous system. This renowned text is virtually unknownin its original form outside the Spanish-speaking world. In a text that reads like a mix between a recipe book and an alchemical manuscript, authors Miguel Merchan, Javier DeFelipe, and Fernando de Castro (descendant of the 1933 publication's author) put the new translation into historical context. This book is also beautifully illustrated with plates ofhistological techniques, provides a quick guide to new vocabulary, and the author's notes on the translated text. This pivotal work of classic neurohistological techniques is a wonderful addition to the Cajal library.

About The Author

Miguel A. Merhcan is a Professor of Histology at the University of Salamanca in Spain. Javier De Felipe is Profesor de Investigacion at the Instituto Cajal (Consejo Superior de Investigaciones Cientificas) and Centro de Tecnologia Biomedica in Madrid, Spain. Fernando de Castro is a member of the Instituto Cajal, Grupo de Neurobiologia...

Details & Specs

Title:Cajal and de Castros Neurohistological MethodsFormat:HardcoverDimensions:304 pages, 9.25 × 6.12 × 0.98 inPublished:March 16, 2016Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0190221593

ISBN - 13:9780190221591

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Table of Contents

Principles of Micrographical Techniques for the Nervous SystemDedicationPreliminary InformationList of PlatesSection I: Historical Context1. Jaime A. Merchan and Jorge Prieto: Authors and Their BackgroundsSection II: Basic Histological Techniques2. The Microscope and the FixativesI. MicroscopeII. FixativesFormaldehyde-based FixativesFixatives with Osmic AcidFixatives Based on Hypnotic Substances3. Decalcifying Agents4. General Dissociation ProceduresDissociationSectioningFrozen Section Procedure (EN 8)Celloidin and Collodion EmbeddingParaffin EmbeddingGelatin EmbeddingSuperficial Paraffin Imbedding5. Handling and Mounting Serial SectionsSections of celloidin-embedded materialSections of paraffin-embedded material6. General Staining MethodsSome Remarks on the Staining ProcessSources and Composition of Dyes7. Formulae for Carmine and HaematoxylinDouble and Triple StainsModifications of the Iron-Haematoxylin. Methods for the Staining of Mitochondria8. Section Mounting and PreservationMounting Sections that Require Previous Dehydration and ClarifyingSection Mounting without Dehydration and ClarifyingSection III: Special Techniques9. Methods for the Demonstration of Neuronal Morphology10. Continuation of the Methods for Demonstrating the Morphology of NeuronsFixation, Sectioning and Mounting Techniques for Methylene Blue Stained Material11. Methods to Study the Structure of the Nerve CellNissl Chromatic BodiesMethods for Staining the NeurofibrilsSilver Impregnation MethodsImpregnation Of The Golgi ApparatusDemonstration of Plastosomes, Centrosome, Oxidases and Pigmentary SpherulesNucleus and Cell Membrane12. Staining of Neuronal Axons in the Centres13. Myelin Staining14. Colouration of the Macroglia, Microglia and OligodendrogliaMacrogliaDemonstration of the Gliosomes and MitochondriaImpregnation of Oligodendroglia and Microglia15. Methods to Demonstrate the Connective TissueMethods For Staining The Connective TissueImpregnation Methods for Connective TissueSupravital Staining with Acidic Dyes16. Methods for the Demonstration of Substances Produced by Alterations of the Cell MetabolismDemonstration of some Cell GranulesDemonstration of Fats and Lipoid SubstancesDemonstration of Ferric PigmentsDemonstration of non-Ferric PigmentsGlycogen DemonstrationDemonstration of Amyloid Substance and Corpora AmylaceaDemonstration of Hyaline DegenerationCalcareous Substances17. Methods for the Demonstration of the Peripheral Nerves under both Normal and Pathological ConditionsDemonstration of Ranvier NodesMethods to Stain the Schwann CellsMethods for Myelin StainingLantermann Incisures; Spiral Apparatus; Spinous Double Bracelet; Neurokeratin Network (EN 48)Demonstration of the Cylinder-Axis and NeurofibrilsDemonstration of the Connective Tissue of Nerve Trunks (Endoneurium, Perineurium and Epineurium)18. Methods for the Demonstration of Peripheral Nerve EndingsGold Chloride MethodsDemonstration of Nerve Endings with the Golgi MethodDemonstration of Peripheral Nerve Endings with Ehrlich's Methylene Blue ProcedureNeurofibrillary Methods Capable of Demonstrating the Nerve EndingsMethods to Demonstrate the Nerve Trunks and Nerve Endings in Organs Protected by Bone TissueProcedures for Staining the Sympathetic and Dorsal Root Ganglia19. Techniques for the Demonstration of the Nervous Tissue of InvertebratesMethods Capable of Revealing the Morphology of NeuronsMethods for Staining Neurofibrils20. Methods for Demonstrating some Pathogenic MicroorganismsDemonstration of the Syphilis TreponemaDemonstration of the Trypanosomes in Sleeping SicknessColouration of Negri Bodies in RabiesDemonstration of the Tuberculosis BacillusEditors' NotesVocabulary by the EditorsBibliography