Calcium Measurement Methods by Verkhratsky AlexeiCalcium Measurement Methods by Verkhratsky Alexei

Calcium Measurement Methods

EditorVerkhratsky Alexei

Paperback | February 25, 2012

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Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signaling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In "Calcium Measurement Methods", a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific applications of the technique to neural tissues. The book's contents show how the combination of different indicators and recently developed microscopic techniques lead to spectacular successes in the imaging of neural cells in the living brain in both physiological and pathophysiological models. As a volume in the successful NeuromethodsT series, the chapters provide authoritative reviews including up-to-date detailed protocols of the most commonly used approaches in the field.Cutting-edge and concise, "Calcium Measurement Methods" serves as an ideal reference to aid the many scientists worldwide engaged in calcium imaging of the nervous system.
Title:Calcium Measurement MethodsFormat:PaperbackDimensions:268 pagesPublished:February 25, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617796778

ISBN - 13:9781617796777

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Table of Contents

1. Principles of the Ca2+ Homeostatic/Signalling SystemAlexei Verkhratsky and Ole H. Petersen2. Ca2+ Recordings: Hardware and Software (From Microscopes to Cameras)Emil C. Toescu and Jeremy Graham3. Ca2+ Imaging: Principles of Analysis and Enhancement Fabio Mammano and Mario Bortolozzi4. Bioluminescent Ca2+ IndicatorsLaura Fedrizzi and Marisa Brini5. Monitoring Calcium Levels With Genetically Encoded IndicatorsOlga Garaschuk and Oliver Griesbeck6. Intracellular Calcium-Sensitive MicroelectrodesRoger C. Thomas7. Ca2+ Caging and UncagingShin Hye Kim and Myoung Kyu Park8. Ca2+ Imaging of Intracellular Organelles: Endoplasmic ReticulumRobert Blum, Ole H. Petersen, and Alexei Verkhratsky9. Ca2+ Imaging of Intracellular Organelles: MitochondriaLucía Núñez, Carlos Villalobos, María Teresa Alonso, and Javier García-Sancho10. Ca2+ Imaging of Dendrites and SpinesKnut Holthoff11. In vivo Ca2+ Imaging of the Living Brain Using Multi-Cell Bolus Loading TechniqueGerhard Eichhoff, Yury Kovalchuk, Zsuzsanna Varga, Alexei Verkhratsky, and Olga Garaschuk12. Ca2+ Imaging of GliaChristian Lohr and Joachim W. Deitmer