Calcium Signalling: A Practical Approach

Hardcover | December 15, 2000

EditorAlexei Tepikin

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This new edition of Calcium Signalling focuses on the applications of fluorescent and bioluminescent techniques for studying calcium signalling and calcium dependent processes. Cameleon probes, targeting bioluminescent probes, low affinity fluorescence indicators and chelators, and photometrictechniques are all covered in depth. Also described in detail are methods for monitoring mitochondrial function, measuring calcium in the nuclear envelope and nucleoplasm, measuring calcium dependent gene expression, monitoring calcium signals in multicellular preparations, and measuring calciumextrusion. A major problem of fluorescent calcium measurements is the artifacts from zinc and transition metals and a chapter is devoted to their detection and minimization. Some of the pharmacological methods of studying calcium release are also covered. Many of the chapters are written by thescientists who developed the methods they are describing so the authors could not be more qualified. It is hoped that this volume will prove as invaluable to the studying cellular calcium as its predecessor.

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This new edition of Calcium Signalling focuses on the applications of fluorescent and bioluminescent techniques for studying calcium signalling and calcium dependent processes. Cameleon probes, targeting bioluminescent probes, low affinity fluorescence indicators and chelators, and photometrictechniques are all covered in depth. Also d...

Alexei Tepikin is in the Physiological Laboratory, University of Liverpool.
Format:HardcoverPublished:December 15, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199638489

ISBN - 13:9780199638482

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Table of Contents

Chameleons as cytosolic and intra-organellar calcium probesPhotometry, video imaging, confocal and multi-photon microscopy approaches in calcium signalling studiesDetecting and minimizing errors in calcium-probe measurements arising from transition metals and zincTargeting of bioluminescent probes and calcium measurements in different subcellular compartmentsMonitoring mitochondrial function in single cellsUsing low-affinity fluorescence calcium indicators and chelators for monitoring and manipulating free (Ca2+) in endoplasmic reticulum.Measuring calcium in the nuclear envelope and nucleoplasmFrom calcium signal to gene expression. Controlling cytoplasmic calcium and measuring calcium-dependent gene expression in intact cells.Monitoring the generation and propagation of calcium signals in multicellular preparations.Pharmacological studies of new calcium release mechanismsMeasuring calcium extrusionAppendix one - Fluorescent calcium indicators and caged calcium probes

Editorial Reviews

"The book provides frank discussions about the advantages and disadvantages of a wide range of approaches in calcium signalling. It will be essential for new researchers in the field and provides useful new information for established workers. Also, the clearly presented protocols should beeasy to follow in the lab." Trends in Cell Biology