Cancer Cell Culture: Methods and Protocols by Ian A. CreeCancer Cell Culture: Methods and Protocols by Ian A. Cree

Cancer Cell Culture: Methods and Protocols

EditorIan A. Cree

Hardcover | April 25, 2011

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With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success - only by good practice and quality control will the results of cancer cell culture improve.  Written in the successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.
Title:Cancer Cell Culture: Methods and ProtocolsFormat:HardcoverDimensions:516 pagesPublished:April 25, 2011Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617790796

ISBN - 13:9781617790799

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Table of Contents

1. Cancer Biology            Ian A. Cree 2. Principles of Cancer Cell Culture            Ian A. Cree 3. Storage of Cell Lines            Katharine A. Parker 4. Characterization and Authentication of Cancer Cell Lines: An Overview            Yvonne A. Reid 5. Online Verification of Human Cell Line Identity by STR DNA Typing            Wilhelm G. Dirks and Hans G. Drexler 6. Cytogenetic Analysis of Cancer Cell Lines            Roderick A. F. MacLeod and Hans G. Drexler 7. Cell Culture Contamination            Glyn N. Stacey 8. Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction            Cord C. Uphoff and Hans G. Drexler 9. Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics            Cord C. Uphoff and Hans G. Drexler 10. Quality Assurance and Good Laboratory Practice            Louise A. Knight and Ian A. Cree 11. Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning            Laura Breen, Joanne Keenan, and Martin Clynes 12. Isolation and Culture of Colon Cancer Cells and Cell Lines            Sharon Glaysher and Ian A. Cree 13. Isolation and Culture of Melanoma and Naevus Cells and Cell Lines            Julia K. Soo, Alastair D. MacKenzie Ross, and Dorothy C. Bennett 14. Isolation and Culture of Squamous Cell Carcinomalines            Karin J. Purdie, Celine Pourreyron, and Andrew P. South 15. Isolation and Culture of Ovarian Cancer Cells and Cell Lines             Christian M. Kurbacher, Cornelia Korn, Susanne Dexel, Ulrike Schween, Jutta A. Kurbacher, Ralf Reichelt, and Petra N. Arenz 16. Establishment and Culture of Leukemia-Lymphoma Cell Lines            Hans G. Drexler 17. Isolation of Inflammatory Cells from Human Tumors            Marta Polak 18. Isolation of Endothelial Cells from Human Tumors            Elisabeth Naschberger, Vera S. Schellerer, Tilman T. Rau, Roland S. Croner, and Michael Stürzl 19. Cellular Chemosensitivity Assays: An Overview            Venil N. Sumantran 20. Cell Sensitivity Assays: The MTT Assay            J. van Meerlo, G.J.L. Kaspers, and J.Cloos 21. Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay            Sharon Glaysher and Ian A. Cree 22. The Differential Staining Cytotoxicity (DiSC) Assay: A Review            Larry M. Weisenthal 23. Real-time Cytotoxicity Assays            Donald Wlodkowic, Shannon Faley, Zbigniew Darzynkiewicz, and Jonathan M. Cooper 24. FPurification of Annexin V and its use in the Detection of Apoptotic Cells            Katy M. Coxon, James Duggan, M. Francesca Cordeiro, and Stephen E. Moss 25. Measurement of DNA Damage in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay            Janet M. Hartley, Victoria J. Spanswick, and John A. Hartley 26. Molecular Breakpoint Analysis of Chromosome Translocations in Cancer Cell Lines by Long Distance Inverse-PCR            Björn Schneider, Hans G. Drexler, and Roderick A.F. MacLeod 27. Cell Migration and Invasion Assays            K.A. Moutasim, M.L. Nystrom, and G.J. Thomas 28. Angiogenesis Assays            V. Poulaki 29. Flow Cytometric DNA Analysis of Human Cancers and Cell Lines            Sarah A. Krueger and George D. Wilson 30. Expression Analysis of Homeobox Genes in Leukemia/Lymphoma Cell Lines            Stefan Nagel and Hans G. Drexler 31. Measuring Gene Expression from Cell Cultures by Quantitative Reverse-Transciptase Polymerase Chain Reaction            Sharon Glaysher, Francis G. Gabriel, and Ian A. Cree 32. Proteomic Evaluation of Cancer Cells - Identification of Cell Surface Proteins            Samantha Larkin and Claire Hastie 33. Development of Rituximab-resistant B-NHL Clones: An In Vitro Model for Studying Tumor Resistance to Monoclonal Antibody-mediated Immunotherapy            Ali R. Jazirehi and Bejamin Bonavida 34. High Throughput Screening of Natural Products for Cancer Therapy: A Review            Alan L. Harvey and Ian A. Cree 35. Analysis of Drug Interactions            Irene V. Bijnsdorp, Elisa Giovannetti, and Godefridus J. Peters 36. Transfection and DNA-mediated Gene Transfer            Davide Zecchin and Federica Di Nicolantonio 37. Drug Design and Testing: Profiling of Anti-proliferative Agents for Cancer Therapy Using a Cell-based Methyl-[3H]-Thymidine Incorporation Assay            Matthew Griffiths and Hardy Sundaram 38. Feeder Layers: Co-culture with Non-neoplastic Cells            Celine Pourreyron, Karin J. Purdie, Stephen A. Watt, and Andrew P. South 39. Xenotransplantation of Breast Cancers            Massimiliani Cariati, Rebecca Marlow, and Gabriela Dontu