Cancer Stem Cells: Methods and Protocols by John S. YuCancer Stem Cells: Methods and Protocols by John S. Yu

Cancer Stem Cells: Methods and Protocols

EditorJohn S. Yu

Paperback | August 9, 2012

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Through the revolutionary concept of cancer stem cells, cancer research has been reinvigorated to study the role of these unique cells in cancer propagation and as targets of innovative therapies. In Cancer Stem Cells: Methods and Protocols, preeminent researchers have compiled cancer stem cell research techniques and protocols to promote healthy competition, discourse, and collaboration in this vital field. The volume covers extensive topics such as identification and isolation of cancer stem cells, animal models of cancer stem cells, methylation profiling, the contribution of the niche in the regulation of cancer stem cells, immunologic targeting, and the use of normal stem cells as a treatment, among other subjects. Written in the highly successful Methods in Molecular BiologyT series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Cancer Stem Cells: Methods and Protocols will aid scientists around the world in the furthering of our understanding of cancer initiation and propagation and, most importantly, in the development of novel targets for cancer therapy.
Title:Cancer Stem Cells: Methods and ProtocolsFormat:PaperbackDimensions:300 pages, 10.24 × 7.6 × 0.07 inPublished:August 9, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617797537

ISBN - 13:9781617797538

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Table of Contents

1. Defining Cancer Stem Cells by Xenotransplantation in ZebrafishMichael C. Dovey and Leonard I. Zon2. Analysis of Cancer Stem Cell Metastasis in Xenograft Animal ModelsYibin Kang3. Identification of Murine and Human Acute Myeloid Leukemia Stem CellsAniruddha J. Deshpande, Farid Ahmed, and Christian Buske4. Methods for Analysis of Brain Tumor Stem Cell and Neural Stem Cell Self-RenewalIchiro Nakano and Harley I. Kornblum5. Prospective Identification of Cancer Stem Cells with the Surface Antigen CD133 Kyeung Min Joo and Do-Hyun Nam6. Neurosphere Culture and Human Organotypic Model to Evaluate Brain Tumor Stem CellsHugo Guerrero-Cázares, Kaisorn L. Chaichana, and Alfredo Quiñones-Hinojosa7. Methodologies in Assaying Prostate Cancer Stem CellsHangwen Li, Ming Jiang, Sofia Honorio, Lubna Patrawala, Collene R. Jeter, Tammy Calhoun-Davis, Simon W. Hayward, and Dean G. Tang8. Characterization of Non-Malignant and Malignant Prostatic Stem/Progenitor Cells by Hoechst Side Population MethodMurielle Mimeault and Surinder K. Batra9. Anchorage Independent Growth of Prostate Cancer Stem CellsShunyou Wang10. Identification of Human Pancreatic Cancer Stem CellsChenwei Li, C.J. Lee, and Diane M. Simeone11. Cancer Stem Cells in Head and Neck Squamous Cell CarcinomaLaurie Ailles and Mark Prince12. Pituitary Adenoma Stem CellsPatrizia Tunici and John S. Yu13. Genome-Wide DNA Methylation Profiling: The mDIP-Chip TechnologyYin Shen, Shaun D. Fouse, and Guoping Fan14. The Contribution of Niche-Derived Factors to the Regulation of Cancer CellsJulie B. Sneddon15. Dendritic Cell Vaccines for Cancer Stem CellsSerena Pellegatta and Gaetano Finocchiaro16. Maintaining and Engineering Neural Stem Cells for Delivery of Genetically Encoded Therapy to Brain TumorsJennifer Katz, Joseph Krueger, Brunhilde Felding-Habermann, and Evan Y. Snyder17. Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cells Vera S. Donnenberg, E. Michael Meyer, and Albert D. Donnenberg

Editorial Reviews

From the reviews:"This volume in the popular Methods in Molecular Biology series provides a state-of-the art compendium of research techniques and protocols for identifying and culturing rare populations of cancer stem cells. . The audience includes basic science and clinical researchers who are interested in stem cell biology as well as mechanisms of carcinogenesis and malignancy. The book also will be valued by biomedical scientists and clinicians interested in clinical oncology. . The topic is fascinating and clinically relevant." (Bruce A. Fenderson, Doody's Review Service, June, 2010)