Cardiac Cell and Gene Transfer by Joseph M. MetzgerCardiac Cell and Gene Transfer by Joseph M. Metzger

Cardiac Cell and Gene Transfer

EditorJoseph M. Metzger

Paperback | November 10, 2010

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A panel of leading scientific experts detail novel techniques and strategies for the cellular and genetic modification of heart function. The highly experienced authors provide step-by-step protocols for vector production and purification, for gene and cell delivery techniques, and for physiological assessment in vivo and in vitro. Timely, authoritative, and state-of-the-art, Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications constitutes an invaluable guide to all the new cellular and gene-based technologies needed by basic and clinical investigators working to illuminate today's unanswered questions about heart disease and ultimately to improve the heart performance in all their patients.
Title:Cardiac Cell and Gene TransferFormat:PaperbackDimensions:264 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372927

ISBN - 13:9781617372926

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Table of Contents

Part I. Vectors for Cardiac Gene Transfer: Production, Purification, and ApplicationsAdenoviral Vectors: Production and PurificationFaris P. Albayya and Joseph M. MetzgerGutted Adenoviral Vectors for Gene Transfer to MuscleJeannine M. Scott and Jeffrey S. ChamberlainDual Vector Expansion of the Recombinant AAV Packaging CapacityDongsheng Duan, Yongping Yue, and John F. EngelhardtLentivirus Vector-Mediated Gene Transfer to CardiomyocytesTsuyoshi Sakoda, Noriyuki Kasahara, and Larry KedesPart II. Cell Transfer to Myocardium: Protocols and ApplicationsCell Therapy in the Heart: Cell Production, Transplantation, and ApplicationsKevin S. Cahill, Catalin Toma, Mark F. Pittenger, Paul D. Kessler, and Barry J. ByrneCardiac Cell Transplantation: Protocols and ApplicationsSteven M. White and William C. ClaycombCell Grafting for Cardiac RepairHans Reinecke and Charles E. MurryPart III. Vectors for Cardiac Gene Transfer: In Vitro and In Vivo Assays and ApproachesSuppressor tRNAs: Protocols and Applications for Cardiac Gene TransferMassimo Buvoli, Ada Buvoli, and Leslie A. LeinwandAntisense Oligonucleotides: Design, Construction, and Applications to Cardiac Allograft TransferLeora B. Balsam, Douglas N. Miniati, and Robert C. RobbinsTheoretical and Technical Considerations for Gene Transfer into Vascularized Cardiac TransplantsGuanyi Lu and D. Keith BishopIsolation, Culture, and Gene Transfer of Adult Canine Cardiac MyocytesJennifer C. Hirsch, Andrea R. Borton, and Joseph M. MetzgerMyofilament Protein Phosphorylation by PKC in Genetically Engineered Adult Cardiac MyocytesMargaret V. WestfallPart IV. In Vivo Cardiac Gene Transfer: Protocols, Applications, and Physiological AssessmentEmbryonic and Neonatal Cardiac Gene Transfer In VivoGeir Christensen, Peter J. Gruber, Yibin Wang, and Kenneth R. ChienEfficient Viral Gene Transfer to Rodent Hearts In VivoFederica del Monte and Roger J. HajjarDirect Gene Transfer to the Adult Rodent Myocardium In VivoMichael L. Szatkowski, Margaret V. Westfall, and Joseph M. MetzgerAdenovirus-Mediated Gene Transfer to Cardiac Myocytes In Vivo Using Catheter-Based ProceduresDamien Logeart and Jean-Jacques MercadierCoronary Perfusion Cocktails for In Vivo Gene TransferStephan E. Lehnart and J. Kevin DonahueModification of In Vivo Cardiac Performance by Intracoronary Gene Transfer of b-Adrenergic Receptor Signaling ComponentsHendrik T. Tevaearai and Walter J. KochProtocols for Hemodynamic Assessment of Transgenic Mice In VivoDimitrios Georgakopoulos and David A. KassIndex

Editorial Reviews

"The book is surprisingly up-to-date...it is quite unique, as there is no similar compendium of detailed protocols. I found the book to be highly readable. It is a particularly useful reference work for cardiovascular scientists who are already using gene transfer approaches in their research. It is also a great starting place for cardiovascular investigators who are just beginning to use these methods in their labs." -Doody's Health Sciences Book Review Journal