Cell Biological Applications of Confocal Microscopy instructs researchers on methods of preparing biological material for confocal microscopy. Individual chapters describe the technical problems of imaging structures in thick specimens and useful techniques such as multi-color dye visualization. The book provides specific examples of applications of confocal microscopy to cell biological problems. This volume is an essential guide for students and scientists incell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities.
Describes proper specimen preparation for obtaining high-quality data and images
Covers pitfalls in imaging different specimens and in employing different dyes
Contains detailed protocols accessible to new users
Chapters are self-contained units, but describe strategies that can be combined to develop new protocols
Techniques are illustrated with full-color plates