Cell Cycle Control and Dysregulation Protocols by Antonio GiordanoCell Cycle Control and Dysregulation Protocols by Antonio Giordano

Cell Cycle Control and Dysregulation Protocols

EditorAntonio Giordano, Gaetano Romano

Paperback | November 10, 2010

Pricing and Purchase Info

$172.68 online 
$193.95 list price save 10%
Earn 863 plum® points

In stock online

Ships free on orders over $25

Not available in stores

about

Expert laboratorians detail emerging methodologies for studying the cell cycle, its kinases, and kinase inhibitors. The authors focus the issues of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Many of the protocols are new, such as electron microsocopy to detect apoptosis and proteosome-mediated degradation, whereas others study the interactions between protein-protein, protein-DNA, and protein-RNA.
Title:Cell Cycle Control and Dysregulation ProtocolsFormat:PaperbackDimensions:200 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372706

ISBN - 13:9781617372704

Look for similar items by category:

Customer Reviews of Cell Cycle Control and Dysregulation Protocols

Reviews

Table of Contents

Part I. Analysis of Cyclins and Cyclin-Dependent KinasesThe Biology of Cyclins and Cyclin-Dependent Protein Kinases: An IntroductionLucio MieleIn Situ Immunofluorescence Analysis: Immunofluorescence MicroscopyAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinIn Situ Immunofluorescence Analysis: Analyzing RNA Synthesis by 5-Bromouridine-5'-Triphosphate LabelingAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinImmunofluorescence Analysis Using Epitope-Tagged Proteins: In Vitro SystemAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinAnalysis of In Vivo Gene Expression Using Epitope-Tagged ProteinsAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinChromatin ImmunoprecipitationAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinProtein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: Electrophoretic Mobility Shift AssayAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinProtein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: DNase I DigestionAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinProtein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: Ligation-Mediated Polymerase Chain ReactionAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinAssays for Cyclin-Dependent Kinase InhibitorsAdrian M. Senderowicz. Protein Degradation Via the Proteosome, Henry Hoff, Hong Zhang, and Christian SellPart II. Analysis of the Factors Involved in Cell Cycle DeregulationThe Transformed PhenotypeHenry Hoff, Barbara Belletti, Hong Zhang, and Christian SellA Morphologic Approach to Detect Apoptosis Based on Electron MicroscopyMartyn K. White and Caterina CintiDetection of Apoptotic Deoxyribonucleic Acid Break by In Situ Nick TranslationCarmela Trimarchi, Dario La Sala, Alessandra Zamparelli, and Caterina CintiPart III. Cellular Response to Deoxyribonucleic Acid DamageInduction of Deoxyribonucleic Acid Damage by Alkylating AgentsSalvatore Cortellino, David P. Turner, Domenico Albino, and Alfonso BellacosaInduction of Deoxyribonucleic Acid Damage by g IrradiationSalvatore Cortellino, David P. Turner and Alfonso BellacosaUltraviolet Irradiation of CellsDavid P. Turner, Anthony T. Yeung, and Alfonso BellacosaPart IV. Retroviridae-Based Vectors: Protocols for Lentiviral- and Retroviral-Mediated Gene Transfer to Engineer Cell Culture SystemsTransient Production of Retroviral- and Lentiviral-Based Vectors for the Transduction of Mammalian CellsTiziana Tonini, Pier Paolo Claudio, Antonio Giordano, and Gaetano RomanoDetermination of Functional Viral Titer by Drug-Resistance Colony Assay, Expression of Green Fluorescent Protein, and b-Galactoside StainingTiziana Tonini, Pier Paolo Claudio, Antonio Giordano, and Gaetano RomanoRetroviral and Lentiviral Vector Titration by the Analysis of the Activity of Viral Reverse TranscriptaseTiziana Tonini, Pier Paolo Claudio, Antonio Giordano, and Gaetano RomanoPart V. Detection of Gene Expression in Subcellular CompartmentsSingle and Double Colloidal Gold Labeling in Postembedding Immunoelectron MicroscopyNicoletta Zini, Liliana Solimando, Caterina Cinti, and Nadir Mario MaraldiMultifluorescence Labeling and Colocalization AnalysesMassimo Riccio, Maja Dembic, Caterina Cinti, and Spartaco SantiIndex

Editorial Reviews

"State-of-the-art and highly practical...offers pathbreaking researchers powerful tools for probing the cell cycle regulation across a wide variety of pathologies." - Tumori