Cell Imaging Techniques: Methods and Protocols by Douglas J. TaatjesCell Imaging Techniques: Methods and Protocols by Douglas J. Taatjes

Cell Imaging Techniques: Methods and Protocols

EditorDouglas J. Taatjes, Jürgen Roth

Hardcover | October 2, 2012

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Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of Cell Imaging Techniques: Methods and Protocols expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters  covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Cell Imaging Techniques: Methods and Protocols, Second Edition is an easily accessible volume of protocols to be used with a variety of imaging-based equipment likely available in a core imaging facility.
Title:Cell Imaging Techniques: Methods and ProtocolsFormat:HardcoverDimensions:523 pages, 10 × 7.01 × 0.01 inPublished:October 2, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627030557

ISBN - 13:9781627030557

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Table of Contents

1.  Digital Images are Data - and Should be Treated as SuchDouglas W. Cromey 2. Epi-fluorescence MicroscopyDonna J. Webb and Claire M. Brown3. Live-Cell Migration and Adhesion Turnover AssaysLacoste, J. Young, K. and Brown, C. M.4. Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of HemostaticProteins in Human Venous ValvesWinifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill5. Colocalization Analysis in Fluorescence MicroscopyJeremy Adler6. A Time-Lapse Imaging Assay to Study Nuclear Envelope BreakdownSunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman7. Light Sheet Microscopy in Cell BiologyRaju Tomer, Khaled Khairy and Philipp J. Keller 8. Image-based High-throughput Screening for Inhibitors of Angiogenesis Lasse Evensen, Wolfgang Link, and James B. Lorens9. Intravital Microscopy to Image Membrane Trafficking in Live RatsAndrius Masedunskas, Monika Sramkova, Laura Parente and Roberto Weigert10. Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential Interference Contrast Microscopy and Planar Illumination Microscopy Wei Sun, Lehui Xiao, and Ning Fang 11. Laser Scanning Cytometry. Principles and Applications: An UpdatePiotr Pozarowski , Elena Holden, and Zbigniew Darzynkiewicz12. Laser Capture Microdissection for Protein and NanoString RNA AnalysisYelena Golubeva, Rosalba Salcedo, Claudius Mueller, Lance A. Liotta, and Virginia Espina 13. Viewing Dynamic Interactions of Proteins and a Model Lipid Membrane with Atomic Force Microscopy Anthony S. Quinn,  Jacob H. Rand,  Xiao-Xuan Wu,  and Douglas J. Taatjes 14. Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force MicroscopyLuda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko 15. Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy IndentationJoanna L. MacKay and Sanjay Kumar16. Atomic Force Microscopy Functional Imaging on Vascular Endothelial CellsLilia A. Chtcheglova and Peter Hinterdorfer 17. Porosome: The Secretory NanoMachine in CellsBhanu P. Jen 18. Stereology and Morphometry of Lung TissueChristian Mühlfeld, Lars Knudsen, Matthias Ochs 19. A Novel Combined Imaging/Stereological Method for the Analysis of Human Sural Nerve Biopsies for Clinical DiagnosisMichele A. VonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes 20. Correlative Light-Electron Microscopy as a Tool to Study In vivo Dynamics and uUtrastructure of Intracellular StructuresElena V. Polishchuk, Roman S. Polishchuk and Alberto Luini21. Photooxidation Technology for Correlative Light and Electron MicroscopyClaudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller, Margit Pavelka, Adolf Ellinger 22. Electron Microscopy of Endocytic PathwaysCarmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, Margit Pavelka 23. Morphological Analysis of AutophagyKeisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori 24. Cytochemical Detection of Peroxisomes and MitochondriaNina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader 25. Histochemical Detection of Lipid Droplets in Cultured CellsMichitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto 26. Environmental Scanning Electron Microscopy in Cell BiologyJ. E. McGregor, L. T. L. Staniewicz,  S. E. Guthrie (neé Kirk) and A. M. Donald 27. Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell BiologyF. Rosso, F. Papale and A. Barbarisi 28. High Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular OrganellesPaul Walther, Eberhard Schmid, Katharina Höhn 29. MALDI Imaging Mass Spectrometry for Direct Tissue AnalysisStephan Meding and Axel Walch 27. Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell BiologyF. Rosso, F. Papale and A. Barbarisi 28. High Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular OrganellesPaul Walther, Eberhard Schmid, Katharina Höhn 29. MALDI Imaging Mass Spectrometry for Direct Tissue AnalysisStephan Meding and Axel Walch 27. Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell BiologyF. Rosso, F. Papale and A. Barbarisi 28. High Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular OrganellesPaul Walther, Eberhard Schmid, Katharina Höhn 29. MALDI Imaging Mass Spectrometry for Direct Tissue AnalysisStephan Meding and Axel Walch 27. Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell BiologyF. Rosso, F. Papale and A. Barbarisi 28. High Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular OrganellesPaul Walther, Eberhard Schmid, Katharina Höhn 29. MALDI Imaging Mass Spectrometry for Direct Tissue AnalysisStephan Meding and Axel Walch 27. Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell BiologyF. Rosso, F. Papale and A. Barbarisi 28. High Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular OrganellesPaul Walther, Eberhard Schmid, Katharina Höhn 29. MALDI Imaging Mass Spectrometry for Direct Tissue AnalysisStephan Meding and Axel Walch