Cell Imaging Techniques by Douglas J. TaatjesCell Imaging Techniques by Douglas J. Taatjes

Cell Imaging Techniques

EditorDouglas J. Taatjes

Paperback | November 10, 2010

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A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.
Title:Cell Imaging TechniquesFormat:PaperbackDimensions:504 pages, 9.02 × 5.98 × 0.01 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161737394X

ISBN - 13:9781617373947

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Table of Contents

Molecular Beacons: Fluorescent Probes for Detection of Endogenous mRNAs in Living CellsDiana P. BratuSecond-Harmonic Imaging of CollagenGuy Cox and Eleanor KableVisualizing Calcium Signaling in Cells by Digitized Wide-Field and Confocal Fluorescent MicroscopyMichael Wm. Roe, Jerome F. Fiekers, Louis H. Philipson, and Vytautas P. BindokasMultifluorescence Labeling Techniques and Confocal Laser Scanning Microscopy on Lung TissueMaria Stern, Douglas J. Taatjes, and Brooke T. MossmanEvaluation of Confocal Microscopy System PerformanceRobert M. ZuckerQuantitative Analysis of Atherosclerotic Lesion Composition in MiceMarilyn P. Wadsworth, Burton E. Sobel, David J. Schneider, Wendy Tra, Hans van Hirtum, and Douglas J. TaatjesApplications of Microscopy to Genetic Therapy of Cystic Fibrosis and Other Human DiseasesThomas O. Moninger, Randy A. Nessler, and Kenneth C. MooreLaser Scanning Cytometry: Principles and ApplicationsPiotr Pozarowski, Elena Holden, and Zbigniew DarzynkiewiczNear-Clinical Applications of Laser Scanning CytometryDavid A. Rew, Gerrit Woltmann, and Davinder KaurLaser Capture MicrodissectionVirginia Espina, John Milia, Glendon Wu, Stacy Cowherd, and Lance A. LiottaAnalysis of Asbestos-Induced Gene Expression Changes in Bronchiolar Epithelial Cells Using Laser Capture Microdissection and Quantitative Reverse Transcriptase-Polymerase Chain ReactionChristopher B. Manning, Brooke T. Mossman, and Douglas J. TaatjesNew Approaches to Fluorescence In Situ HybridizationSabita K. Murthy and Douglas J. DemetrickMicroarray Image ScanningLatha Ramdas and Wei ZhangNear-Field Scanning Optical Microscopy in Cell Biology and CytogeneticsMichael Hausmann, Birgit Perner, Alexander Rapp, Leo Wollweber, Harry Scherthan, and Karl-Otto GreulichPorosome: The Fusion Pore Revealed by Multiple Imaging ModalitiesBhanu P. JenaSecretory Vesicle Swelling by Atomic Force MicroscopySang-Joon Cho and Bhanu P. JenaImaging and Probing Cell Mechanical Properties With the Atomic Force MicroscopeKevin D. CostaReflection Contrast Microscopy: The Bridge Between Light and Electron MicroscopyF. A. Prins, I. Cornelese-ten Velde, and E. de HeerThree-Dimensional Analysis of Single Particles by Electron Microscopy: Sample Preparation and Data AcquisitionTeresa Ruiz and Michael RadermacherThree-Dimensional Reconstruction of Single Particles in Electron Microscopy: Image ProcessingMichael Radermacher and Teresa RuizA New Microbiopsy System Enables Rapid Preparation of Tissue for High-Pressure FreezingDimitri Vanhecke, Peter Eggli, Werner Graber, and Daniel StuderIndex

Editorial Reviews

From the reviews:"...deserves to do well." -Ultramicroscopy"The book covers a wide range of experimental imaging methods for the investigation of cells. . The book is well produced, with many excellent photographs and diagrams, and I have enjoyed broadening my awareness of techniques that not only did I not know in detail but in some cases not even know existed. A nice book . ." (D. J. Cook, British Journal of Biomedical Science, Vol. 63 (2), 2006)"This 21-chapter volume . provides detailed descriptions of and protocols for a plethora of microscopy and related methods to study tissues, cells and macromolecules. . This comprehensive and well rounded book is suitable for institutional or personal purchase, novices and experts, and it is certainly a must-read for all serious confocal microscopists and imaging facility managers." (Andreas Holzenburg, Microbiology Today, August, 2006)"Douglas Taatjes and Brooke Mossman have edited a compilation of 21 articles. . The subjects covered are very diverse, ranging from light microscopy to electron microscopy. . In summary, this book provides an excellent overview on current and relevant imaging techniques. The described methods and protocols are not only of particular interest to those who are new in the field or who have to expand their imaging capability above regular light microscopy. The book will serve as a reference for years to come . ." (Carsten Schultz, ChemBioChem, Vol. 7, 2006)"This text will be extremely useful. . it is a collection of advanced and sometimes disparate microscopy techniques and their application to biological research. . The authors include highly detailed descriptions and instructions on the low-level methods computer programmers use for acquisition, storage, and manipulation of digital image data." (Steven Ruzin, Microscope, Vol. 54, 2006)