Cell Migration in Inflammation and Immunity: Methods and Protocols by Daniele D'AmbrosioCell Migration in Inflammation and Immunity: Methods and Protocols by Daniele D'Ambrosio

Cell Migration in Inflammation and Immunity: Methods and Protocols

EditorDaniele D'Ambrosio, Francesco Sinigaglia

Paperback | November 10, 2010

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Leading cell biologists and immunologists present their most useful and innovative techniques for studying the molecular and cellular basis of cell migration in the inflammatory process. The authors provide a series of focused, cutting-edge techniques proceeding from the in vitro analysis of cell migration and the molecular mechanisms underlying this process, to methodologies for the analysis of cell migration in vivo. Experimental approaches useful in establishing the role of cell migration in the pathogenesis of both acute and chronic inflammatory diseases are emphasized. Methods for the analysis of rapid leukocyte adhesion under flow conditions in vitro are described, which may prove especially fruitful for scientists exploring the molecular mechanisms underlying vascular recognition and leukocyte-endothelium interaction. Each fully tested protocol includes an introduction explaining the principle behind the technique, equipment and reagent lists, and tips on troubleshooting and how to avoid known pitfalls.
Title:Cell Migration in Inflammation and Immunity: Methods and ProtocolsFormat:PaperbackDimensions:289 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617373575

ISBN - 13:9781617373572

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Table of Contents

Chemotaxis and Interaction with Vascular or Lymphatic EndotheliumSilvano Sozzani, Annunciata Vecchi, Paola Allavena, and Alberto MantovaniAnalysis of Integrin-Dependent Rapid Adhesion Under Laminar-Flow ConditionsCarlo LaudannaPosttranslational Processing of ChemokinesPaul Proost, Frank Mahieu, Evemie Schutyser, and Jo Van DammeChemotactic Profiling of Lymphocyte SubpopulationsLucia Colantonio, Andrea Iellem, and Daniele D'AmbrosioMeasurement of the Levels of Polymerized Actin (F-Actin) in Chemokine-Stimulated Lymphocytes and GFP-Coupled cDNA Transfected Lymphoid Cells by Flow CytometryMiguel Vicente-Manzanares, Mariano Vitón, and Francisco Sánchez-MadridEvaluation of Rho Family Small G-Protein Activity Induced by Integrin Ligation on Human LeukocytesAngela Gismondi, Fabrizio Mainiero, and Angela SantoniReconstructing Leukocyte Migration in 3D Extracellular Matrix by Time-Lapse Videomicroscopy and Computer-Assisted TrackingPeter Friedl and Eva-B. BröckerAnalyzing Chemotaxis Using Dictyostelium discoideum as a Model SystemMark A. Landree and Peter N. DevreotesConditional Transgenic Models to Study Chemokine BiologySergio A. Lira, Borna Mehrad, Shu-Cheng Chen, Petronio Zalamea, David J. Kinsley, Maria T. Wiekowski, Elizabeth Coronel, Galya Vassileva, Denise Manfra, and Kristian K. JensenIntravital Microscopy as a Tool for Studying Recruitment and ChemotaxisDenise C. Cara and Paul KubesTracking Antigen-Specific Lymphocytes In VivoClaire L. Adams, Catherine M. Rush, Karen M. Smith, and Paul GarsideAnalysis of Homing-Receptor Expression on Infiltrating Leukocytes in Disease StatesMargherita Mariani and Paola Panina-BordignonInteraction of Viral Chemokine Inhibitors with ChemokinesAntonio AlcamiDiscovery of Small-Molecule Antagonists of Chemokine Receptors: Screening Strategy and AssaysMaria Elena FuentesVisualization and Analysis of Adhesive Events in Brain Microvessels by Using Intravital MicroscopyGabriela ConstantinAnimal Models to Study Chemokine Receptor Function In Vivo: Mouse Models of Allergic Airway InflammationClare M. Lloyd and Jose-Carlos Gutierrez-RamosAssessing the Role of Multiple Phosphoinositide 3-Kinases in Chemokine Signaling: Use of Dominant Negative Mutants Controlled by a Tetracycline-Regulated Gene Expression SystemAdam P. Curnock, Yannis Sotsios, and Stephen G. WardIn Vitro and In Vivo Models to Study Chemokine Regulation of AngiogenesisGiovanni Bernardini, Domenico Ribatti, Gaia Spinetti, Lucia Morbidelli, Marina Ziche, Angela Santoni, Maurizio C. Capogrossi, and Monica NapolitanoReal Time In Vitro Assay for Studying Chemoattractant-Triggered Leukocyte Transendothelial Migration Under Physiological Flow ConditionsGuy Cinamon and Ronen AlonGeneration of Monoclonal Antibodies Against Chemokine ReceptorsLeonor Kremer and Gabriel MárquezDetection of High-Affinity a4-Integrin Upon Leukocyte Stimulation by Chemoattractants or ChemokinesJason R. Chan and Myron I. CybulskyIndex