Centrosomes and Spindle Pole Bodies

Other | September 1, 2001

byPalazzo, Robert E., Robert E. Palazzo

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Containing a comprehensive collection of convenient and quantitative methods for studying centrosomes, spindle pole bodies and related organelles, this text is a valuable resource for researchers and others interested in studying the role of these organelles in cell replication. Chapters outlining the role of these organelles in other cell functions are also included, and a wide variety of experimental systems for analyzing these organelles are presented. Detailed protocols for experiments are contained in each chapter for researchers to perform in their own labs. This volume outlines key methodologies used to analyze centrosomes and spindle pole bodies, their replication, and reproduction in the clear, well-illustrated style of the Methods in Cell Biology series.

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Containing a comprehensive collection of convenient and quantitative methods for studying centrosomes, spindle pole bodies and related organelles, this text is a valuable resource for researchers and others interested in studying the role of these organelles in cell replication. Chapters outlining the role of these organelles in other ...

Format:OtherDimensions:375 pages, 1 × 1 × 1 inPublished:September 1, 2001Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0080496628

ISBN - 13:9780080496627

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Table of Contents

Contributors.
Preface.
Purification and Reconstitution ofDrosophilia g-- Tubulin Complexes.
Using Rapid Freeze and Freeze-Substitution for the Preparation of Yeast Cells for Electron Microscopy and Three-Dimensional Analysis.
Digital Fluorescence Microscopy of Cell Cytoplasts with and without the Centrosome.
Methods for the Study of Pericentrin in Centrosome Assembly and Function.
Molecular Dissection of Yeast Spindle Pole Bodies by Two Hybridin VitroBinding, and Co-purification.
Genetic Analysis of Yeast Spindle Pole Bodies.
Methods for the Study of Centrosomes inDrosophiliaduring Embryogenesis.
Methods for Identification of Centrosome-Associated Proteins.
Reconstitution of Centrosome Microtubule Nucleation inDrosophilia.
Reconstitution of Centrosome Microtubule Nucleation inSpisula.
In VitroApproaches for the Study of Microtubule Nucleation at the Fission Yeast Spindle Pole Body.
Gamma Tubulin and Microtubule Nucleation in Mammalian Cells.
Gamma Tubulin in Plant Cells.
Centrosomes and Parthenogenesis.
In VitroApproaches for the Study of Molecular Motors in Aster Formation.
Methods for the Study of Centrosome-Independent Spindle Assembly inXenopusExtracts.
Methods for the Study of Centrosome Reproduction in Mammalian Cells.
Centrosome Reproduction inXenopusLysates.
Centrosome Reproductionin Vitro: Mammalian Centrosomes inXenopusLysates.
Identification of Centrosome Kinases.
Methods for the Analysis of Centrosome Reproduction in Cancer Cells.
Isolation of Centrosomes fromDictyostelium.
Index.
Volume in Series.