Checkpoint Controls and Cancer: Volume 2: Activation and Regulation Protocols by Axel H. SchönthalCheckpoint Controls and Cancer: Volume 2: Activation and Regulation Protocols by Axel H. Schönthal

Checkpoint Controls and Cancer: Volume 2: Activation and Regulation Protocols

EditorAxel H. Schönthal

Paperback | November 19, 2010

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Prestigious researchers from ten different countries present readily reproducible experimental protocols to study the molecular components of checkpoint controls and their regulation. Described in step-by-step detail, these cutting-edge techniques offer novel approaches, such as the use of genome databases and siRNA, to analyzing how cells of the human body can escape proper surveillance to grow into a tumor. Additional experimental methods are provided for the manipulation of checkpoint pathways and the analysis of the resulting consequences for the cellular phenotype. An earlier first volume, Reviews and Model Systems, comprehensively reviews the complexities of checkpoint controls and the model systems needed to study them.
Title:Checkpoint Controls and Cancer: Volume 2: Activation and Regulation ProtocolsFormat:PaperbackDimensions:376 pagesPublished:November 19, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617376035

ISBN - 13:9781617376030

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Table of Contents

Part I. Protocols for the Study of Checkpoint-Regulatory ComponentsAnalysis of RB Action in DNA Damage Checkpoint ResponseChristopher N. Mayhew, Emily E. Bosco, David A. Solomon, Erik S. Knudsen, and Steven P. AngusInteraction Between the Retinoblastoma Protein and Protein Phosphatase 1 During the Cell CycleNorbert Berndt and John W. LudlowGeneration of p53 Target Database Via Integration of Microarray and Global p53 DNA-Binding Site AnalysisSuxing Liu, Asra Mirza, and Luquan WangFunctional Analysis of CDK Inhibitor p21WAF1Rati Fotedar, Mourad Bendjennat, and Arun FotedarAnalysis of p21CDKN1A Recruitment to DNA Excision Repair Foci in the UV-Induced DNA Damage ResponseLucia A. Stivala and Ennio ProsperiQuantitative Determination of p16 Gene Expression by RT-PCRSylke Schneider, Kazumi Uchida, Dennis Salonga, Ji Min Yochim, Kathleen D. Danenberg, and Peter V. DanenbergMeasuring Cyclin-Dependent Kinase ActivityAxel H. SchönthalDetermination of the Catalytic Activities of mTOR and Other Members of the Phosphoinositide-3-Kinase-Related Kinase FamilyGary G. Chiang and Robert T. AbrahamCHK1 Kinase Activity AssayYa Wang and Hongyan WangAssaying Cdc25 Phosphatase ActivityIngo Hassepass and Ingrid HoffmannAnalyzing the Regulation and Function of ATMMartin F. Lavin, Shaun P. Scott, Sergei Kozlov, and Nuri GuevenUse of siRNA to Study the Function of MDC1 in DNA Damage ResponsesZhenkun Lou and Junjie ChenFunctional Analysis of APC-Cdh1Tamotsu Sudo, Naoto T. Ueno, and Hideyuki SayaPurification of Mitotic Checkpoint Complex, an Inhibitor of the APC/C From HeLa CellsValery Sudakin and Tim J. YenAnalysis of the Spindle-Assembly Checkpoint in HeLa CellsPaul R. Andreassen, Dimitrios A. Skoufias, and Robert L. MargolisFunctional Analysis of the Spindle-Checkpoint Proteins Using an In Vitro Ubiquitination AssayZhanyun Tang and Hongtao YuPart II. Studying Consequences of Checkpoint Pathway ActivationAnalysis of Checkpoint Responses to Histone Deacetylase InhibitorsHeather Beamish, Robyn Warrener, and Brian G. GabrielliBiochemical Analysis of the Cell Cycle and Cell Cycle Checkpoints in Transiently Transfected Cells After Collection With Magnetic BeadsXiaofen Ye, Maxim Poustovoitov, Hidelita Santos, David M. Nelson, and Peter D. AdamsAnalysis of DNA Repair and Chromatin Assembly In Vitro Using Immobilized Damaged DNA SubstrateJill A. Mello, Jonathan G. Moggs, and Geneviève AlmouzniAnalyzing Cell Cycle Checkpoints After Ionizing RadiationBo Xu and Michael B. KastanFACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic CellsWilliam R. TaylorAnalysis of Cell Cycle by Flow CytometryPiotr Pozarowski and Zbigniew DarzynkiewiczAnalyzing Markers of Apoptosis In VitroStéphanie Plenchette, Rodolphe Filomenko, Emmanuelle Logette, Stéphanie Solier, Nelly Buron, Séverine Cathelin, and Eric SolaryAnalysis of Telomerase Activity and Telomere Function in CancerKatrina E. Gordon and E. Kenneth ParkinsonIndex