Chemical Neurobiology: Methods and Protocols by Matthew R. BanghartChemical Neurobiology: Methods and Protocols by Matthew R. Banghart

Chemical Neurobiology: Methods and Protocols

EditorMatthew R. Banghart

Hardcover | March 16, 2013

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Many advances in modern neuroscience are enabled by the availability of chemical tools that allow sensitive, precise, and quantitative measurements of, and control over, biological processes. These powerful reagents are widely used for investigating the nervous system at levels of detail ranging from ion channel structure to neural network dynamics. Recent advances in photochemistry, microscopy, and protein engineering have triggered a surge in the development and application of these interdisciplinary techniques. Chemical Neurobiology: Methods and Protocols assists with the design, characterization and validation of new chemical tools for neurobiology by providing detailed protocols of procedures and assays deemed essential for the successful development and implementation of such tools. Divided into three sections, topics cover chemical probes of membrane protein structure and function, photochemical control of protein and cellular function, and chemical probes for imaging in the nervous system. Written in the successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Chemical Neurobiology: Methods and Protocols serves scientists at many levels, including students aiming to expand their perspective, laboratory researchers seeking technical guidance, and established investigators looking for creative solutions to their research problems in molecular, cellular and systems neuroscience.
Title:Chemical Neurobiology: Methods and ProtocolsFormat:HardcoverDimensions:212 pagesPublished:March 16, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627033440

ISBN - 13:9781627033442

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Table of Contents

Part I. Chemical Probes of Membrane Protein Structure and Function 1. Engineering K+ Channels using Semisynthesis Alexander G. Komarov, Corey A. Costantino, and Francis I. Valiyaveetil 2. Chemical Derivatization and Purification of Peptide-Toxins for Probing Ion Channel Complexes Zhengmao Hua and William R. Kobertz 3. Using Yeast to Study Potassium Channel Function and Interactions with Small Molecules Sviatoslav N. Bagriantsev and Daniel L. Minor Jr. 4. A FLIPR Assay for Evaluating Agonists and Antagonists of GPCR HeterodimersJessica H. Harvey, Richard M. van Rijn, and Jennifer L. Whistler Part II. Photochemical Control of Protein and Cellular Function 5. Characterizing Caged Molecules Through Flash Photolysis and Transient Absorption Spectroscopy Joseph P. Y. Kao and Sukumaran Muralidharan 6. Characterization of One- and Two- Photon Photochemical Uncaging Efficiency Alexandre Specht, Frederic Bolze, Jean Francois Nicoud, and Maurice Goeldner 7. Photochromic Potassium Channel Blockers: Design and Electrophysiological Characterization Alexandre Mourot, Timm Fehrentz, and Richard H. Kramer 8. A 1H NMR Assay for Measuring the Photostationary States of Photoswtichable LigandsMatthew R. Banghart and Dirk Trauner 9. Developing a Photoreactive Antagonist Pamela M. England Part III. Chemical Probes for Imaging in the Nervous System 10. Development and in vitro Characterization of Ratiometric and Intensity-based Fluorescent Ion Sensors Laurel A. Cooley, Vladimir V. Martin, and Kyle R. Gee 11.  Characterization of Voltage-Sensitive Dyes in Living Cells using Two-Photon Excitation Corey D. Acker and Leslie M. Loew            12. Characterization and Validation of Fluorescent Receptor Ligands: A Case Study of the Ionotropic Serotonin ReceptorRuud Hovius 13.  Imaging Single Synaptic Vesicles in Mammalian Central Synapses with Quantum Dots Qi Zhang 14.  Directed Evolution of Protein-Based Neurotransmitter Sensors for MRI  Philip A. Romero, Mikhail G. Shapiro, Frances H. Arnold, and Alan Jasanoff