Chemokine Protocols by Amanda E.I. ProudfootChemokine Protocols by Amanda E.I. Proudfoot

Chemokine Protocols

EditorAmanda E.I. Proudfoot, Timothy N.C. Wells, Christine Power

Paperback | November 10, 2010

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Expert investigators describe in detail the most widely used techniques in chemokine biology. Covering both ligands and receptors, these readily reproducible methods cover all aspects of chemokine research, ranging from the cloning and characterization of chemokines and their receptors, through the use of animal models to study chemokine function in vivo. Each method also includes relevant background information, as well as providing a useful bibliography that renders the study of chemokines accessible at all levels of experience. Comprehensive and highly practical, Chemokine Protocols offers experimental and clinical chemokine researchers today's gold-standard collection of proven methods for analyzing this biologically ubiquitous and important class of proteins.
Title:Chemokine ProtocolsFormat:PaperbackDimensions:332 pages, 9.02 × 5.98 × 0.07 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617371513

ISBN - 13:9781617371516

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Table of Contents

Purification of Chemokines from Natural Sources, Jens-M. Schröder. Cloning of Novel Chemokines Using a Signal Sequence Trap Method, Toshio Imai. Chemokine Expression in Insect Cells, Toshio Imai. Expression of Chemokines in Escherichia coli, Michael D. Edgerton, Lars-Ole Gerlach, Thomas P. Boesen, and Bernard Allet. Expression of Chemokines in the Periplasmic Space of E. coli, Jochen Pfirstinger and Matthias Mack. Synthesis of Chemokines, Ian Clark-Lewis. Identification of Novel Chemokines from Expressed Sequence Tag Databases, Timothy N. C. Wells and Manuel C. Peitsch. Purification of Recombinant Chemokines from E. coli, Amanda E. I. Proudfoot and Frédéric Borlat. Chemokine Receptor Cloning, Philip M. Murphy. Generation of Stable Cell Lines Expressing Chemokine Receptors, Christine A. Power and Alexandra Meyer. Modified Microchemotaxis Assays, Dennis D. Taub. Transwell Chemotaxis, Paul D. Ponath, Juan Wang, and Heidi Heath. Endothelial Cell Chemotaxis Assays, Darcey Black. Radiolabeled Chemokine Binding Assays, Bruce L. Daugherty, Salvatore J. Siciliano, and Martin S. Springer. Scintillation Proximity Binding Assay, Sami Alouani. Calcium Mobilization, Raphaële Buser and Amanda E. I. Proudfoot. Actin Polymerization, Jörn Elsner and Alexander Kapp. Reactive Oxygen Release, Jörn Elsner and Alexander Kapp. Histamine Release, Michael A. Lett-Brown and Rafeul Alam. Measurement of Phosphoinositide 3-Kinase Activity, Stephen G. Ward. Glycosaminoglycan Binding Assays, Arlene J. Hoogewerf and Gabriele S. V. Kuschert. CFU-A Assay for Measurement of the Antiproliferative Effects of Chemokines on Murine Early Hemopoietic Progenitors, Gerard J. Graham and Mary G. Freshney. Downmodulation and Recycling of Chemokine Receptors, Matthias Mack and Detlef Schlöndorff. Analysis of Chemokine Receptor Endocytosis and Recycling, Nathalie Signoret and Mark Marsh. Chemokine Inhibition of HIV Infection, Jacqueline D. Reeves and Graham Simmons. The Production of Chemokine Specific Monoclonal Antibodies: Chemokine RANTES, Peter J. Nelson. Monoclonal Antibodies to Chemokine Receptors, Paul D. Ponath, Nassim Kassam and Shixin Qin. Targeted Expression of Chemokines In Vivo, Iqbal Grewal, Long Gu, Susan Tseng, and Barrett J. Rollins. Chemokine Knockout Mice, Marc E. Rothenberg. Chemokine Receptor Knockout Mice, Ji-Liang Gao and Philip M. Murphy. Measurement of Eosinophil Accumulation In Vivo, Maria-Jesus Sanz, Peter J. Jose, and Timothy J. Williams. Murine Model of Allergic Lung Inflammation, Sami Alouani, Pierre Juillard, and Yolande Chvatchko. Murine Models of Airway Inflammation, Emma M. Campbell and Nicholas W. Lukacs. Rat Models of Respiratory Inflammation, Andrew S. McWilliam and Patrick G. Holt. Murine Model of Crescentic Nephritis, Clare Lloyd and Jose-Carlos Gutierrez-Ramos. Rabbit Models of Pneumonia, Peritoneal Sepsis, and Lung Injury, Charles W. Frevert, Gustavo Matute-Bello, and Thomas R. Martin. Index.

Editorial Reviews

highly recommended...Seldom does on find so much valuable information, concisely presented and with such obvious potential for the future, made available in one volume." - E-Streams"...Chemokine Protocols is a collection of clear, well written protocols and I would recommend it as an excellent starting point for any scientist with an interest in chemokines." - Molecular Biotechnology