Chromatin: A Practical Approach

Paperback | April 1, 1998

EditorHannah Gould

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Chromatin comprises the complex of nucleic acids and proteins that go to make up the chromosomes, and regulation of its structure is increasingly recognized as an important factor in gene expression. Researchers from related fields needing an introduction to the methodology of chromatin willfind this book invaluable, as will experienced workers.

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Chromatin comprises the complex of nucleic acids and proteins that go to make up the chromosomes, and regulation of its structure is increasingly recognized as an important factor in gene expression. Researchers from related fields needing an introduction to the methodology of chromatin willfind this book invaluable, as will experience...

Hannah Gould is at King's College, London.

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Format:PaperbackDimensions:248 pages, 9.21 × 6.14 × 0.55 inPublished:April 1, 1998Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199635986

ISBN - 13:9780199635986

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Table of Contents

1. Isolation and fractionation of chromatin and linker histones2. Extraction and separation of core histones and non-histone chromosomal proteins3. Mapping chromatin structure in Drosophila4. In vivo footprint and chromatin analysis by LMPCR5. Analysis of nuclear scaffold attachment regions6. Electron microscopy of chromatin7. Counting nucleosome cores on circular DNA using topoisomerase I8. In vitro reconstitution of chromatin and analysis of nucleosome positioning9. Transcriptional and structural analysis of chromatin assembled in vivo10. An assembly of chromatin and nuclear structures in Xenopus egg extractsAppendix: List of SuppliersIndex

Editorial Reviews

"The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methodscapable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately thenatural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and establishedinvestigators will make use of this volume."--The Quarterly Review of Biology