Chromatographic Methods by A. BraithwaiteChromatographic Methods by A. Braithwaite

Chromatographic Methods

byA. Braithwaite

Paperback | October 13, 2011

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In recent years the techniques of chromatography have progressed rapidly. However, the aims and objectives of the First Edition, as quoted below, are just as relevant today as they undoubtedly were in 1963. 'The various methods of separating mixtures which are grouped under the general name chromatography are now well known and widely used. Since the inception of chromatography as a column technique in 1903, the principal landmarks in its progress have been its virtual rediscovery in the 1930s, the invention of synthetic resins in 1935, the introduction of paper chromatography in the early 1940s and finally, the development of gas solid and gas liquid chromatography in the late 1940s and early 1950s. Subsequent expansion in the use of chromatographic methods has been rapid and continuous, with the result that in the last 15 years a substantial volume of literature on the subject has appeared, dealing not only with particular separations but also in much specific detail with improvements in technique.
Title:Chromatographic MethodsFormat:PaperbackDimensions:9.25 × 6.1 × 0.68 inPublished:October 13, 2011Publisher:Springer NetherlandsLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:9401083169

ISBN - 13:9789401083164

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Table of Contents

1 Introduction.- 1.1 Introduction to chromatography.- 1.2 Historical aspects of chromatography.- 1.3 Classification of chromatographic methods.- References.- 2 Theoretical considerations.- 2.1 Factors influencing retention.- 2.2 Theory of separations and retention characteristics.- 2.3 Column efficiency.- 2.4 Band broadening.- 2.5 Resolution.- 2.6 Quantitation in chromatography.- References.- 3 Plane chromatography.- 3.1 Thin-layer chromatography (TLC).- 3.2 Paper chromatography (PC).- 3.3 Electrophoresis.- References.- 4 Liquid phase chromatography on open columns.- 4.1 Practical aspects/considerations.- 4.2 Modes of chromatography.- 4.3 Adsorption chromatography.- 4.4 Partition column chromatography.- 4.5 Ion-exchange chromatography.- 4.6 Salting out chromatography.- 4.7 Inorganic ion-exchangers.- 4.8 Liquid ion-exchangers.- 4.9 Gel ion-exchangers.- 4.10 Gel chromatography.- 4.11 Affinity chromatography.- 4.12 Covalent chromatography.- 4.13 Chromatofocusing.- References.- 5 Gas chromatography (GC).- 5.1 Principles of GC.- 5.2 GC instrumentation.- 5.3 Sampling techniques.- 5.4 The column and column oven.- 5.5 Detectors for GC.- 5.6 Capillary column gas chromatography.- 5.7 Applications of GC.- 5.8 Gas-solid chromatography.- References.- 6 High performance liquid chromatography (HPLC).- 6.1 Modes of chromatography.- 6.2 Overview of HPLC instrumentation.- 6.3 Theory.- 6.4 Detailed discussion of HPLC instrumentation.- 6.5 Column packings and stationary phases for LC.- 6.6 Adsorption chromatography.- 6.7 Liquid-liquid partition chromatography (LLC).- 6.8 Chemically-bonded stationary phases (BP) for HPLC.- 6.9 Chiral stationary phases.- 6.10 Ion-exchange chromatography (IEC).- 6.11 Ion pairing.- 6.12 Size exclusion chromatography.- 6.13 LC method development.- 6.14 Quantitative analysis.- 6.15 Preparative liquid chromatography (PLC).- 6.16 Microcolumns in liquid chromatography.- 6.17 Applications of HPLC.- References.- 7 Spectroscopic techniques and chromatography.- 7.1 Chromatographic requirements.- 7.2 Mass spectroscopy-chromatographic techniques (GC-MS, HPLC-MS).- 7.3 Infrared spectrophotometry.- 7.4 UV-visible spectrophotometry.- 7.5 Atomic spectroscopy.- References.- 8 Processing of chromatographic data.- 8.1 Methods of recording the chromatographic signal.- 8.2 Data collection and processing.- References.- 9 Model experiments in chromatographic techniques.- 9.1 List of experiments.- 9.2 Section A. Paper chromatography.- 9.3 Section B. Electrophoresis and related techniques.- 9.4 Section C. Thin-layer chromatography.- 9.5 Section D. Column chromatography.- 9.6 Section E. Gas chromatography.- 9.7 Section F. High performance liquid chromatography (HPLC).- References.