Chromosome Structural Analysis: A Practical Approach

Paperback | April 1, 1999

EditorWendy Bickmore

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The DNA of eukaryotes is packaged into chromosomes - each chromosome consisting of a very long molecule of DNA and various proteins (e.g. histones), and the number of chromosomes being characteristic for the species concerned. Chromosome analysis can provide a great deal of information formany aspects of cellular genetics such as DNA replication, protein:DNA interactions and genetic manipulation. The book is structured in a methodical fashion - the introductory chapters are centred around analysis of chromatin with chapters on the mapping of protein:DNA interactions in vivo usingligation-mediated PCR and the mapping of chromatin-associated proteins by formaldehyde cross-linking. The next chapters concentrate on the study of whole chromosome structure, including: fission yeast chromosome analysis using FISH and CHIP, isolation of vertebrate metaphase chromosomes and theiranalysis by FISH, the study of vertebrate chromosome progression through mitosis, and the analysis of mammalian interphase chromosomes by immunofluorescence and FISH. There then follow chapters on FISH in whole-mount tissues and the analysis of the sub-structure of mammalian nuclei in vitro. Thefinal two chapters deal with the experimental manipulation of chromosome structure, including: chromosome assembly in vitro using Xenopus egg extracts and chromosome fragmentation in vertebrate cell lines. This comprehensive and informative laboratory manual includes a diverse range of experimentalmodels for the analysis of chromosomes - such as vertebrates, Drosophila, yeast and Xenopus. Fully illustrated, it focuses on modern techniques and approaches to the study of chromosome structure and will be invaluable to researchers and academic staff in genetics, biomedical science and molecularbiology.

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From the Publisher

The DNA of eukaryotes is packaged into chromosomes - each chromosome consisting of a very long molecule of DNA and various proteins (e.g. histones), and the number of chromosomes being characteristic for the species concerned. Chromosome analysis can provide a great deal of information formany aspects of cellular genetics such as DNA r...

Dr Wendy A. Bickmore, Cell Genetics Section, MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU. Tel: 0131 332 2471. Fax: 0131 343 2620. Email: wendy@hgu.mrc.ac.uk
Format:PaperbackPublished:April 1, 1999Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199636982

ISBN - 13:9780199636983

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Table of Contents

1. Mapping protein:DNA interactions in vivo using ligation-mediated Polymerase Chain Reaction2. Mapping DNA target sites of chromatin-associated proteins by formaldehyde cross-linking in Drosophila embryos3. Fission yeast chromosome analysis: Fluorescence in situ hybridization (FISH) and chromatin immunoprecipitation4. Isolation of vertebrate metaphase chromosomes and their analysis by FISH5. Studying progression of vertebrate chromosomes through mitosis by immunoflourescence and FISH6. Analysis of mammalian interphase chromosomes by FISH and immunofluorescence7. Fluorescence in situ hybridization in whole-mount tissues8. Analysing the sub-structure of mammalian nuclei, in vitro9. Chromosome assembly in vitro using Xenopus egg extracts10. Chromosome fragmentation in vertebrate cell lines

Editorial Reviews

"...The book is an essential read for all workers in fields such as molecular biology, genetics and biomedical science whose work involves chromosome analysis." Clinical Laboratory International, September 1999