Cilia, Part A

Other | March 12, 2013

byWallace F. Marshall, Wallace F. Marshall

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This new volume ofMethods in Enzymologycontinues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.



  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers cilia
  • Contains chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium

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This new volume ofMethods in Enzymologycontinues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium.Con...

Wallace Marshall is an electrical engineer by training, who became interested in biology out of a desire to understand how cells solve engineering problems, such as determining the size of organelles. He received his Ph.D. at UCSF with John Sedat, where he studied the diffusional of motion of interphase chromatin using live cell imagin...

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Format:OtherDimensions:472 pages, 1 × 1 × 1 inPublished:March 12, 2013Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0123984866

ISBN - 13:9780123984869

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Table of Contents


    1. Electron microscopy of IFT in cilia and flagella
    2. Biochemical analysis of ciliary transport
    3. Visualization of IFT in Chlamydomonas flagella
    4. Visualization of kidney cilia-mediated calcium signaling
    5. Methods for analyzing nodal cilia and flow
    6. Methods for building synthetic cilia-like motor-microtubule arrays
    7. Isolation of IFT particle proteins from Chlamydomonas
    8. Radial Spoke isolation and assays
    9. Biomechanical measurements of kinocilium
    10. Methods for analysis of Calcium/calmodulin signaling in flagella
    11. Ubiquitination of Flagellar Proteins
    12. Visualizing IFT in C. elegans cilia
    13. Proteomic analysis of mammalian primary cilia
    14. TSC and TOR signaling in cilia
    15. Bioinformatic approaches to cilia
    16. Methods for testing cilia function in cell migration
    17. Signaling and waveform in Drosophila sperm flagella
    18. Analysis of import into primary cilia
    19. Patch-Clamp Recording from Mouse and Human Spermatozoa
    20. Optical trap measurements of flagellar membrane motility