Circulating MicroRNAs: Methods and Protocols by Takahiro OchiyaCirculating MicroRNAs: Methods and Protocols by Takahiro Ochiya

Circulating MicroRNAs: Methods and Protocols

EditorTakahiro Ochiya

Hardcover | May 30, 2013

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microRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols,  expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.

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Title:Circulating MicroRNAs: Methods and ProtocolsFormat:HardcoverDimensions:281 pages, 10 × 7.01 × 0.1 inPublished:May 30, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627034528

ISBN - 13:9781627034524

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Table of Contents

1. Functional Analysis of Exosomal MicroRNA in Cell-Cell Communication ResearchNobuyoshi Kosaka, Yusuke Yoshioka, Keitaro Hagiwara, Naoomi Tominaga and Takahiro Ochiya  2.  Isolation of Extracellular Nanovesicle MicroRNA from Liver Cancer Cells in Culture  Takayuki Kogure and Tushar Patel 3. Methods of Analysis of Dendritic Cell-derived Exosome-shuttle MicroRNA and its Horizontal Propagation Between Dendritic CellsAngela Montecalvo, Adriana T. Larregina, and Adrian E. Morell 4. Analysis of MicroRNA and Protein Transfer by Exosomes During an Immune SynapseCarolina Villarroya-Beltri, Cristina Gutiérrez-Vázquez, Francisco Sánchez-Madrid, and María Mittelbrunn 5. Analysis of Viral MicroRNA Exchange via Exosomes In Vitro and In VivoFrederik J Verweij, Monique AJ van Eijndhoven, Jaap Middeldorp and D Michiel Pegtel 6. Measurement of Precursor miRNA in Exosomes from Human ESC-Derived Mesenchymal Stem CellsTian Sheng Chen and Sai Kiang Lim 7. Analysis of the Transfer of Circulating microRNA Between Cells Mediated by Gap JunctionSteven J. Greco, Pranela Rameshwar 8. Isolation of Circulating MicroRNA Associated with RNA-binding ProteinAndrey Turchinovich, Ludmila Weiz,  and Barbara Burwinkel 9. Identification and Analysis of Circulating Exosomal microRNA in Human Body FluidsCecilia Lässer 10. Analyzing the Circulating MicroRNAs in Exosomes/Extracellular Vesicles from Serum or Plasma by qRT-PCRLeni Moldovan, Kara Batte, Yijie Wang, Jon Wisler, and Melissa Piper 11. Direct Serum Assay for MicroRNA in Cancer PatientsSota Asaga  and Dave S.B. Hoon 12. Analysis of MicroRNA Niches: Techniques to Measure Extracellular MicroRNA and Intracellular MicroRNA In SituVictoria N. Parikh and Stephen Y. Chan 13. Analysis of Circulating MicroRNA by Microarray in Liver Disease Yoshiki Murakami and Toshihito Tanahashi  14. Isolation of Circulating MicroRNA in SalivaAlessia Gallo, Ilias Alevizos 15. Purification of RNA From Milk WheyHirohisa Izumi, Nobuyoshi Kosaka, Takashi Shimizu, Kazunori Sekine, Takahiro Ochiya, and Mitsunori Takase 16. Circulating MicroRNAs in the Cerebrospinal Fluid of Patients with Brain DiseasesAkira Machida, Takuya Ohkubo, and Takanori Yokota 17. Methods of MicroRNA Quantification in Urinary SedimentWang Gang, and Szeto Cheuk Chun,  18. Circulating microRNA for the Identification of Forensically Relevant Body FluidsErin K. Hanson and Jack Ballantyne 19. Identification of Prostate Cancer-associated MicroRNAs in Circulation using a Mouse Model of DiseaseLuke A. Selth, Scott Townley, Joanna Gillis, Wayne Tilley and Lisa Butler 20. A Combination of Extraction Reagent and DNA Microarray that Allows for the Detection of Global MiRNA Profiles from Serum /PlasmaMakiko Ichikawa and Hideo Akiyama 21. Nanopore Single-molecule Detection of Circulating microRNAsLi-Qun Gu and Yong Wang