Colorectal Cancer: Methods and Protocols by Steven M. PowellColorectal Cancer: Methods and Protocols by Steven M. Powell

Colorectal Cancer: Methods and Protocols

EditorSteven M. Powell

Paperback | November 9, 2010

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Steven M. Powell, MD, has assembled a hands-on collection of cutting-edge molecular methods for studying colorectal tumors. These readily reproducible techniques range from gross microdissection of specimens to specific molecular analyses. Here both the novice and experienced researcher will find productive chromosomal analyses, including comparative genomic hybridization and FISH assays, and revealing gene expression analyses, including immunohistochemical assays, Western blotting, and microarray assays. Eminently practical, with each technique presented as if the expert in the field who had perfected it were guiding the investigator, Colorectal Cancer: Methods and Protocols provides cancer investigators with a gold standard collection of all the key genetic, cellular, and molecular biological methods for analyzing colon cancer today.
Title:Colorectal Cancer: Methods and ProtocolsFormat:PaperbackDimensions:294 pagesPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617371793

ISBN - 13:9781617371790

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Table of Contents

Microdissection of Histological Sections: Manual and Laser Capture Microdissection TechniquesChristopher A. MoskalukIsolation of a Purified Epithelial Cell Population from Human ColonJames K. RocheXenografting Human Colon CancersJeffrey C. Harper, Reid B. Adams, and Steven M. PowellComparative Genomic Hybridization TechniqueWa'el El-Rifai and Sakari KnuutilaFluorescence In Situ Hybridization: Application in Cancer Research and Clinical DiagnosticsSvetlana D. Pack and Zhengping ZhuangMicrosatellite Analysis to Assess Chromosome 18q Status in Colorectal CancerJin JenThe SURF Technique: Selective Genetic Analysis of Microscopic Tissue HeterogeneityDarryl ShibataMicrosatellite Instability TestingYann R. Parc and Kevin C. HallingImmunohistochemical Analysis for hMLH1 and hMSH2 Expression in Colorectal CancerKevin C. Halling and Patrick C. RocheMutation Detection in Colorectal Cancers: Direct Sequencing of DNA Mismatch Repair GenesJulie M. Cunningham, David J. Tester, and Stephen N. ThibodeauMutation Detection in the TGF-b ReceptorsWilliam M. Grady, Lois L. Myeroff, Hongmei He, and Sanford MarkowitzDirect Analysis for Familial Adenomatous Polyposis MutationsSteven M. PowellI1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal CancerPerry S. Chan and Steven L. GersenMicrosatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal CarcinomasRhonda F. Souza and Stephen J. MeltzerMolecular Detection of Smad2/Smad4 Alterations in Colorectal TumorsSam ThiagalingamDirect Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 MutationsLauri A. Aaltonen and Stina RothDirect Sequencing for Peutz-Jehgers Gene LKB1 (STK11) MutationsLauri A. Aaltonen and Egle AvizienyteDirect Sequencing for Cowden Syndrome Gene PTEN (MMAC1) MutationsLauri A. Aaltonen, Stina Roth, and Charis EngGenetic Analysis Using MicroarraysWa'el El-Rifai and Sakari KnuutilaCyclooxygenase-2 (COX-2) Protein Expression by Western BlottingHossam M. Kandil and Raymond N. DuBoisThe Immunohistochemical MethodLisa A. Cerilli and Henry F. Frierson, JrImmunohistochemical Detection and Quantitation of Cell Surface Receptors for ProstanoidsRosana Cosme and James K. RocheAdhesion Signaling Through IntegrinsReid B. Adams and Joshua D. RovinElectrophoretic Mobility Shift Assay (EMSA)Michael F. Smith, Jr. and Sandrine Delbary-GossartTransfection Assays for Transformation of Model Colonic Cell LinesMark T. Worthington and Roger Qi LuoAssessment of Intestinal Stem Cell Survival Using the Microcolony Formation AssayKimberly S. Tustison, Joanne Yu, and Steven M. CohnGene Transfer into the Colonic MucosaBaçak Çoruh and Theresa T. PizarroIndex

Editorial Reviews

"This is quite interesting and a valuable volume. It aims to instruct investigators in the key genetic, cellular and molecular biological methods for analyzing colorectal tumors. It's quite precise in its description of these techniques and, as the preface says, "The focused techniques and assays are described in sufficient detail to allow researchers to start an experiment on colon tumors and proceed from beginning to end as if the expert in the field who has performed these studies were guiding them at the bench." A large amount of practical information is packed into one small volume and this volume is ideal for students and fellows who wish to expand their knowledge in this area and have a ready volume reference in developing new techniques...The volume is also good value for those of us who don't get into the lab as it clarifies many of the techniques which are used and helps the non-involved reader to understand the terminology and techniques used in this important area of cancer research." - Cancer Forum "...Dr. Saltz has produced as concise a presentation as possible within the framework of the widely scattered disciplines searching the complexity of diagnostic and therapeutic problems occurring among patients with colorectal cancer." - Comprehensive Therapy