Complement: A Practical Approach

Paperback | July 3, 1997

EditorAlister W. Dodds, Robert B. Sim

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The complement system is a collection of plasma enzymes that interact in a complex way as a reaction to cell injury, and is central to the immune response. The enzymes that make up the system and their interactions are very widely studied. This volume provides detailed practical protocols forquantitation of the complement components, inhibitors, and receptors, and methods for analysis of complement biosynthesis and for the study of complement in non-human species.

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The complement system is a collection of plasma enzymes that interact in a complex way as a reaction to cell injury, and is central to the immune response. The enzymes that make up the system and their interactions are very widely studied. This volume provides detailed practical protocols forquantitation of the complement components, i...

Dr Alastair Dodds MRC Immunochemistry Unit Dept of Biochemistry University of Oxford South Parks Road Oxford OX1 3QU Tel: 01865 275795 Fax: 01865 275729 Email: al@bioch.ox.ac.uk Dr Robert Sim MRC Immunochemistry Unit Dept of Biochemistry University of Oxford South Parks Road Oxford OX1 3QU Tel: 01865 275351 Fax: 01865 275729

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Format:PaperbackDimensions:296 pages, 9.21 × 6.14 × 0.67 inPublished:July 3, 1997Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199635390

ISBN - 13:9780199635399

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Table of Contents

1. The complement system: an introduction2. Haemolytic assays for whole complement activity and individual components3. Immunochemical methods for the detection of complement components and complement activation4. Identification, functional assessment, and quantitation of receptors and membrane regulatory proteins whose ligands are C3 and C45. C1 inhibitor and HANe6. Formation and regulation of the membrane attack complex7. Purification of complement components8. The anaphylatoxins9. Complement genetics10. Biosynthesis of complement components11. Complement and immune complexes12. Non-human complement

Editorial Reviews

"To those who are not actively engaged in the study of its biology and function, the complement system has remained one of the most formidable and dreaded subjects to deal with. . . . This manual . . . is therefore a very timely compilation of all the important protocols of assays that deal with thecomplement system. The book's twelve chapters are written by the most respected authorities in the field, and each chapter will serve the interests of researchers and clinicians. In addition, the book contains a useful list of suppliers and their addresses. . . . This compendium of differentprotocols for the isolation, purification and the study of complement structure and function will be a much needed manual, and an easy-to-read companion for both beginners and seasoned veterans. With such a wealth of information, this book provides an easily accessible reference source forclinicians, immunologists and students."--The Quarterly Review of Biology